EMAGE mouse gene expression spatial database

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EMAGE:14422

Gadd45a growth arrest and DNA-damage-inducible 45 alpha ( MGI:107799)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004250. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004250_01	euxassay_004250_02	euxassay_004250_03	euxassay_004250_04

euxassay_004250_05	euxassay_004250_06	euxassay_004250_07	euxassay_004250_08	euxassay_004250_09

euxassay_004250_10	euxassay_004250_11	euxassay_004250_12	euxassay_004250_13	euxassay_004250_14

euxassay_004250_15	euxassay_004250_16	euxassay_004250_17	euxassay_004250_18	euxassay_004250_19

euxassay_004250_20	euxassay_004250_21	euxassay_004250_22	euxassay_004250_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14422_wholemount_strong.wlz

14422_wholemount_moderate.wlz

14422_wholemount_weak.wlz

14422_wholemount_possible.wlz

14422_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14422_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

retina

moderate

regional

moderate expression: see section 01 02 03 04 05 23

nasal cavity olfactory epithelium

moderate

regional

moderate expression: see section 08 09 10 11 12 14 15 16 17 18 19

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7183
Entity Detected:	Gadd45a, growth arrest and DNA-damage-inducible 45 alpha ( MGI:107799)
Sequence:	sense strand is shown >T7183 GGCAGAGTTCCCCAGCGAGGCTAGGCGAGCAGCCGGCCGGCCGGAGCGGAGAAGGGAGGGTGGGAGCGAG CGCAGAGCCGGCGCCGCGCACTGTGGGGGCCAGGAGCAGCCCGCGCGCCGAGGGAGGGACTCGCACTTGC AATATGACTTTGGAGGAATTCTCGGCTGCAGAGCAGAAGACCGAAAGGATGGACACGGTGGGCGATGCCC TGGAGGAAGTGCTCAGCAAGGCTCGGAGTCAGCGCACCATTACGGTCGGCGTGTACGAGGCTGCCAAGCT GCTCAACGTAGACCCCGATAACGTGGTACTGTGCCTGCTGGCTGCTGACGAAGACGACGACCGGGATGTG GCTCTGCAGATCCATTTCACCCTCATCCGTGCGTTCTGCTGCGAGAACGACATCAACATCCTGCGGGTCA GCAACCCGGGTCGGCTAGCTGAGCTGCTGCTACTGGAGAACGACGCGGGCCCGGCGGAGAGCGGGGGCGC CGCGCAGACCCCGGACCTGCACTGTGTGCTGGTGACGAACCCACATTCATCACAATGGAAGGATCCTGCC TTAAGTCAACTTATTTGTTTTTGCCGGGAAAGTCGCTACATGGATCAGTGGGTGCCCGTGATTAATCTCC CGGAACGGTGATGGCATCCGAATGGAAATAACTGAACCAAATTGCACTGAAGTTTTGAAATACCTTTGTA GTTACTCAAGCAGTCACTCCCCACGCTGATGCAAGGATTACAGAAACTGATGTCAAGGGGCCGAGTTCAA CTGCACGAGGGCTCAGAGATGACTTTGCAGAGGGAGAGAGAGGTGAGCCTGAAGAAGGAAGCTGCGAGAA AAGAGAAATCCAAGGCAAAAGGGACAAAAACTACAAAGCACTGCAAGAAAGAAAACTGCTAATTTAGGAT GGCCAGGTTACTTTCAAATAAGCCAAATATTGCTTTGTTGAAACTTTAAATGTATAGCAATAGTTTGGGT ATTTTTTTTCTTTTTTTTTTTTGGTCTTTATGCCCTCAAATAAAAGGAAAGTAAAAGAGGATTAATCATA TTTTCAAGCCACAGTTTAAATGTATTTTGATGAGATGTTAAATTCTCAGAAGTTTTATTATAAATCTTAC TAAGTTATTTTATGATGTGAAAGGTTATTTATGATAAAGTTTTTGAAGCACATTATCTAAAATAAACTGG TATGGAATAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4035898 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4035898 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14419 same embryo
	EMAGE:14423 same embryo
	EMAGE:14421 same embryo
	EMAGE:14418 same embryo
	EMAGE:14420 same embryo
	EurExpress:euxassay_004250 same experiment
	MGI:4824972 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source