EMAGE mouse gene expression spatial database

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EMAGE:14436

Unk unkempt homolog (Drosophila) ( MGI:2442456)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004148. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004148_01	euxassay_004148_02	euxassay_004148_03	euxassay_004148_04

euxassay_004148_05	euxassay_004148_06	euxassay_004148_07	euxassay_004148_08	euxassay_004148_09

euxassay_004148_10	euxassay_004148_11	euxassay_004148_12	euxassay_004148_13	euxassay_004148_14

euxassay_004148_15	euxassay_004148_16	euxassay_004148_17	euxassay_004148_18	euxassay_004148_19

euxassay_004148_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14436_wholemount_strong.wlz

14436_wholemount_moderate.wlz

14436_wholemount_weak.wlz

14436_wholemount_possible.wlz

14436_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14436_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T5615
Entity Detected:	Unk, unkempt homolog (Drosophila) ( MGI:2442456)
Sequence:	sense strand is shown >T5615 GACAGGACCATGTCGAAGGGCCCCGGGCCCGGCGGCTCGGCAGCTTCCTCGGCGCCCCCGGCCGCTACCG CTCAGGTGCTGCAGGCACAGCCCGAGAAACCGCAGCACTACACGTACCTGAAGGAATTCCGCACAGAGCA GTGCCCACTCTTCGTGCAGCACAAATGCACACAGCATCGGCCCTATACCTGCTTCCACTGGCACTTTGTG AACCAGCGTCGCCGCCGTTCCATCCGCCGACGGGATGGCACCTTCAACTACAGTCCAGATGTCTACTGCA CGAAGTATGACGAGGCTACAGGCCTCTGCCCCGAGGGCGACGAGTGCCCATTCCTGCACAGAACCACTGG GGACACTGAGCGCAGGTATCACCTTCGTTACTATAAAACTGGAATCTGCATCCATGAGACAGACTCAAAA GGCAACTGCACCAAAAATGGCCTGCACTGCGCTTTTGCCCACGGGCCTCATGACCTCCGCTCCCCTGTTT ACGACATCAGGGAGCTCCAGGCCATGGAGGCCTTGCAGAATGGCCAGACTACAGTAGAGGGCAGCATAGA AGGCCAGTCAGCTGGGGCTGCAAGCCATGCCATGATAGAAAAAATCCTCAGTGAGGAACCCCGGTGGCAA GAGACTGCTTATGTGCTGGGGAACTATAAGACGGAGCCGTGCAAGAAGCCTCCACGGCTGTGCCGCCAGG GCTATGCCTGTCCCTACTACCACAACAGCAAGGACCGGCGTCGGAGTCCCCGGAAGCACAAATACAGGTC GTCTCCGTGTCCAAACGTCAAGCATGGGGATGAGTGGGGAGACCCTGGCAAGTGTGAAAACGGAGACGCC TGCCAGTATTGCCACACACGCACAGAGCAGCAGTTCCACCCAGAGATCTACAAGTCCACCAAGTGTAATG ACATGCAGCAGGCGGGCAGCTGCCCCCGAGGACCTTTCTGCGCCTTCGCCCACATAGAACCGCCACCCTT AAGTGATGATGTGCAGCCTTCCTCAGCTGTGTCCAGCCCTACCCAGCCAGGTCCCGTCTTGTACATGCCA TCTGCCGCTGGAGACTCGGTCCCTGTGAGCCCCTCCAGCCCGCATGCCCCTGACCTCAGCGCCCTCCTCT GTAGGAATAGTGGCCTGGGCAGTCCGTCTCACCTCTGCAGCTCCCCACCAGGCCCCAGCAGGAAGGCCTC AAACCTGGAGGGTCTGGTCTTCCCTGGGGAGTCCAGCCTTGCCCCTGGCAGCTACAAGAAAGCTCCTGGC TTCGAAAGGGAAGACCAGGTTGGGGCAGAGTACCTGAAGAATTTCAAGTGCCAGGCTAAATTAAAACCCC ATTCGCTAGAGCCCAGGAGTCAAGAGCAGCCTCTGCTTCAACCCAAACAGGACGTGCTGGGCATCCTCCC TGTGGGCAGCCCCCTGACTTCCAGCATCTCTTCCAGTATTACCTCCAGCTTGGCAGCCACTCCCCCCAGC CCTGCAGGCACCAACAGCACCCCTGGCATGAATGCAAATGCTCTGCCCTTCTACCCAACCAGCGACACGG TAGAGTCGGTCATAGAGTCTGCCTTGGATGACCTGGACCTGAATGAGTTTGGAGTGGCTGCCCTAGAGAA GACTTTTGATAACAGCGCAGTGCCCCACCCCAGCAGCGTCACAATCGGTGGCAGTTTGTTGCAGAGCTCC GCGCCTGTGAACATCCCTGGCTCCCTGGGCAGCTCAGCTTCCTTCCACTCTGCTTCTCCATCCCCTCCCG TCAGCCTCTCCTCACACTTCCTGCAGCAGCCCCAGGGCCACTTGAGTCAGTCAGAAAACACGTTTTTGGG GACCTCAGCATCACATGGATCTTTGGGTCTGAACGGGATGAACAGCAGCATCTGGGAGCATTTTGCCTCT GGAAGCTTCTCCCCAGGCACTTCCCCTGCCTTCCTATCAGGGCCAGGGGCTGCTGAGCTGGCCCGGCTTC GGCAAGAGCTAGATGAAGCTAACGGCACCATCAAGCAGTGGGAGGAGTCCTGGAAGCAGGCCAAGCAGGC TTGTGATGCCTGGAAGAAGGAAGCGGAGGAGGCTGGTGAGCGGGCCAGTGCAGCCGGAGCAGAGTGTGAG CTGGCCCGGGAGCAGCGGGATGCCCTGGAGCTGCGGGTGAAGAAGCTGCAGGAGGAGCTGGAGCGGCTGC ACACGGTGCCAGAAGCCCAGACCCTGCCAGCCGCCCCTGACCTGGAGGCGCTGTCCCTCTCCACCCTGTA CTCCATCCAGAAGCAGCTGCGGGTCCACCTGGAACAAGTGGACAAGGCTGTGTTCCACATGCAGTCAGTG AAATGCCTTAAGTGTCAGGAGCAGACCCGGGCAGTGCTGCCGTGCCAACACGCTGTTCTGTGTGAGCTCT GTGCTGAGGGCAGTGAGTGCCCCGTCTGCCAGCCCAGCCGGGCCCATGCCCTCCAGTCGTGACCCTGCAG ACCGGGCCCAGCTGGGCCTAGACCTTTTCACCTAGGACTTTTTAAAGTATATATATGTATGTATGTATGT ATGTACGTATGTATGTATATGTATATGATTATGTACATGTATACATTTCTGTGTGCAGATGCGCGTGGTG CCAGCGTGTACAGTGTCTGTGTGTATCTGGACAGGTGCAGACACACACTTTGAAGCAGACTTACCAAGCA CTTTTTAAAGAAGAGACTATTTTGCAGTCCTCCTGTTCCTTCCCCATCCTTTCTGCTCAAAGACAACGTC CCCTCTTTTCCCACTGGCCTTTTGACACTGAATATGTTTTGTCTCATTTTTTATGTAGGAACTAACTATT TTTAACTTCTTCCTGGGGCCAGAGCAGGGAAGGCTGGGAAGCTGGAAGGGCAGCTCCGTTCACCCTGCCT TTCCAGGCCAGAGGCGTCAGCGTGGACTCAGGTCCCCACTTCCCAGCTGTGAGGGCTTCAGGCAGGTCTG AAACTCTAAACCAGACTAGCTCCTGGGCTTACCAAATATGTATTTAACTCTGGGGACAGCCACAGAGCAG GGAGGCCAAAGCTACAGGCTAGCCCCACTCCCACACCTGGGCCCTTGAAGCTCCTCAAGGCCTAGTTCTG TCCCTCAGTGTTTTGAAACCTGGGCTTCTCTTTGATCACTTCCTTCTGGAATCCCTAGATAGCCTCTTTG ACCCCACAGTCACTTGTCAGGTTGGCACTGCCCTCACCTGTCCCTGCCTCCTTCCCTCTGGCACACGGCC GTAGAGCCGGCCGCTCCCTCCCTCTGGCAGCTAGCAGGGCAATTGTTGGGAGGAGTGCAGTATGAGGACC AAGGGGCACGAGCCCTGCCCTTCTTGCTGGGAGGAGGTAGTATGCATTCCTTGGTGCTCTCAGAGTGCAG CTGACGTACTGCCGTGCTGGGCAGACGCCTGTGTGCATGTGTGTGCGTGCCTGTCTGATGTGTTTAGCTT CAATTTTAAAAATTGTTCTGTACAGAGATGCAGTGAATCGGGAAAGCAGAGTGGGCAGAGGGAGGCAGCC CTGTTCCCAGTACTGGGTGTCCAGAGGGCTCAAGAGGGCTACAAAGAATCTGCCCCAGTAGAGGCCCCTC CCTTGGGCTCAGCACGAAAGGGCTTTCAATGAATTACGTGAAAACTTTCTCCTTTTTTACAAAAATGCAA AAATCAACAAACTTATTGGAAATAAACTATGAACTTGCAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6491704 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6491704 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EurExpress:euxassay_004148 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source