EMAGE mouse gene expression spatial database

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EMAGE:14443

Matn2 matrilin 2 ( MGI:109613)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004287. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004287_01	euxassay_004287_02	euxassay_004287_03	euxassay_004287_04

euxassay_004287_05	euxassay_004287_06	euxassay_004287_07	euxassay_004287_08	euxassay_004287_09

euxassay_004287_10	euxassay_004287_11	euxassay_004287_12	euxassay_004287_13	euxassay_004287_14

euxassay_004287_15	euxassay_004287_16	euxassay_004287_17	euxassay_004287_18	euxassay_004287_19

euxassay_004287_20	euxassay_004287_21	euxassay_004287_22	euxassay_004287_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14443_wholemount_strong.wlz

14443_wholemount_moderate.wlz

14443_wholemount_weak.wlz

14443_wholemount_possible.wlz

14443_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14443_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 16 17 moderate expression: see section 15 18 19 20

mesenchyme

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

diencephalon meninges

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

diencephalon roof plate

strong

regional

strong expression: see section 16 17

choroid invagination

strong

regional

strong expression: see section 09 10 11 12 13 14 moderate expression: see section 15 18 19 20 21

telencephalon meninges

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

hindbrain meninges

moderate

regional

moderate expression: see section 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

metencephalon part of 4th ventricle choroid plexus

strong

regional

strong expression: see section 05 06 07 08 09 10 11 12 13 14 16 17 moderate expression: see section 15 18 19 20

midbrain meninges

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22

spinal cord meninges

moderate

regional

moderate expression: see section 10 11 12 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6091
Entity Detected:	Matn2, matrilin 2 ( MGI:109613)
Sequence:	sense strand is shown >T6091 CTGCGGTCCTCTGCGGGGCGAGGCGGCGGACCACGGAGACTGACACTCACAGCTGCTCCCCTGTCTCTCT TCCCGGGTGTCGGCGGCGGTGGCTGCAGCTGCAGCCCTGCTCCTCTTGAAGATGGAGAAGATGTTGGTGG GATGCTTGCTGATGCTTGGGCAGCTCTTCCTTGTCCTCCCAGTTGACGGCAGAGAGCGTCCCCAAGCCAG GTTCCCTTCCAGAGGCCGTCATGTCCGGATGTATCCGCAGACTGCACTGCTGGAGAGTTCCTGTGAGAAT AAGCGGGCAGATCTGGTGTTCATCATTGACAGCTCCCGAAGTGTCAACACCCATGACTATGCAAAGGTCA AGGAGTTCATTCTGGATATCCTGCAGTTCTTGGACATCGGTCCTGATGTTACCCGAGTAGGTCTGCTCCA ATATGGCAGCACGGTCAAGAACGAGTTCTCCCTGAAGACCTTTAAGAGGAAGTCTGAGGTGGAGAGGGCT GTCAAGAGAATGAGGCATCTATCCACTGGCACCATGACAGGGCTTGCCATCCAATATGCCCTCAACATTG CCTTCTCAGAAGCAGAGGGGGCCAGGCCCTTGCGGGAAAATGTGCCACGCATCATAATGATTGTGACTGA TGGGAGGCCTCAGGACTCGGTAGCTGAGGTAGCTGCCAAGGCCCGGAACACAGGCATCCTGATCTTTGCC ATCGGTGTCGGTCAGGTGGACCTGAATACACTAAAGGCCATTGGGAGTGAGCCCCACAAGGACCATGTCT TCCTGGTGGCCAATTTCAGCCAGATTGAGTCCTTGACCTCAGTGTTCCAGAACAAACTGTGCACAGTCCA CATGTGCAGCATCTTGGAACACAACTGTGCCCACTTCTGCCTCAACACACCTGGCTCGTACATCTGCAAA TGCAAGCAAGGGTACATTCTCAGCACGGATCAGAAGACTTGCAGAATCCAGGATTTGTGTGCCACAGAGG ACCATGGTTGTGAGCAGCTGTGCGTGAACATGCTGGGTTCCTTTGTCTGCCAGTGCTACAGTGGCTACAC CTTGGCTGAGGACGGGAAGCGATGTACCGCTGTGGACTACTGTGCCTCAGAAAATCATGGATGTGAACAC GAGTGTGTAAATGCTGAAAGTTCCTACCTGTGCCGGTGCCACGAGGGATTTGCTCTTAACAGCGATAAGA AAACATGCTCAAAGATTGACTATTGTGCCTCTTCAAATCACGGATGTCAGCATGAGTGTGTCAATGCCCA GACGTCTGCTCTCTGCCGCTGCCTGAAAGGCTTCATGCTAAATCCAGACAGGAAAACTTGCCGAAGGATC AACTACTGTGCACTGAACAAACCAGGCTGTGAGCATGAGTGTGTGAACACAGAGGAGGGTCACTACTGCC GCTGCCGTCAGGGCTACAATCTGGACCCCAATGGCAAGACCTGCAGCCGGGTGGACCACTGTGCACAGCA GGACCATGGCTGTGAGCAGCTGTGCCTGAACACAGAGGAGTCCTTTGTCTGCCAGTGCTCAGAAGGCTTC CTTATTAATGATGACCTCAAGACCTGCTCCAGGGCAGATTACTGCTTGCTGAGCAACCATGGTTGTGAAT ACTCCTGCGTCAACACAGACAAATCCTTTGCCTGCCAATGTCCTGAGGGTCATGTGCTCCGAAGCGATGG GAAGACCTGTGCAAAACTGGACTCTTGTGCTTTGGGGGACCATGGTTGTGAACATTCATGTGTAAGCAGT GAAGACTCTTTCGTGTGTCAGTGCTTTGAGGGGTACATTCTTCGTGACGATGGGAAGACCTGCAGAAGGA AAGATGTCTGCCAGGATGTTAACCATGGATGTGAGCACCTTTGTGTGAACAGTGGCGAATCATACGTCTG CAAGTGTCTGGAGGGGTTCAGGCTGGCTGAGGATGGGAAGCGCTGCAGGAGGAAGAATGTCTGCAAGTCA ACCCAGCATGGCTGTGAGCACATGTGTGTGAACAATGGGAACTCCTATCTGTGCAGGTGCTCAGAGGGCT TTGTCCTAGCTGAGGATGGAAAGCACTGCAAGAGATGCACTGAAGGCCCAATTGACCTGGTCTTTGTGAT TGATGGATCCAAGAGCCTTGGAGAAGAGAACTTTGAGACTGTGAAGCACTTTGTCACTGGAATCATAGAT TCCTTGGCAGTTTCCCCCAAAGCTGCTCGTGTGGGGCTGCTGCAGTATTCCACACAGGTCCGAACAGAGT TTACCCTGAGGGGCTTCAGCTCAGCCAAGGAAATGAAGAAAGCCGTGGCCCACATGAAATACATGGGCAA AGGCTCTATGACTGGGCTGGCCCTGAAACACATGTTTGAGAGAAGTTTTACCCAAGTAGAAGGGGCCAGG CCCCCCTCCACGCAGGTACCCAGAGTAGCCATCGTGTTCACCGACGGACGGGCTCAGGATGATGTCTCTG AGTGGGCCAGTAAAGCCAAGGCCAATGGTATCACTATGTATGCCGTTGGGGTAGGAAAAGCCATTGAGGA AGAACTACAGGAGATTGCCTCTGAGCCCATTGACAAGCATCTCTTCTACGCTGAAGACTTCAGCACAATG GGCGAAATAAGTGAAAAGCTGAAAGAAGGCATCTGTGAAGCTCTAGAAGACTCTGGTGGAAGACAGGACT CAGCAGCATGGGACCTGCCACAGCAGGCCCACCAGCCAACAGAACCTGAGCCAGTCACCATAAAGATCAA AGACCTACTTTCCTGTTCTAATTTTGCAGTGCAACATAGATTCCTGTTTGAAGAAGACAATCTTTCACGG TCTACACAAAAACTTTTCCATTCAACCAAATCTTCAGGAAACCCTTTGGAAGAAAGCCAGGACCAATGCA AATGTGAAAACCTTATACTGTTCCAGAACGTTGCAAATGAAGAAGTAAGAAAATTAACCCAGCGCTTAGA AGAAATGACTCAAAGAATGGAAGCTTTGGAAAATCGCCTAAAATACAGATGAAGACCAGAATTGCTTTTA TGTATATTTGTAAACCATCTTAAAAAGGATTTTGACAAAGGTGGTAGAGCCTCCCACCATAAGCAGACCT ACAAGCTGTAAAGCAAAATACAACCAGTACAGGAGACACTGGATTGCTTGACTGCTGGACAGAGAAAATG GAGACACTGAGGTGTCTAAATTCAATGAGGGTGGGGGGAAGGAATCCTGAATTCTGAGTTTACCATGAGA ATAAATAAGTTGCGCAGTGAAAAAAAAAAAAACAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3492881 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3492881 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14445 same embryo
	EMAGE:14447 same embryo
	EMAGE:14448 same embryo
	EMAGE:14444 same embryo
	EMAGE:14446 same embryo
	EurExpress:euxassay_004287 same experiment
	MGI:4826112 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source