EMAGE mouse gene expression spatial database

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EMAGE:14461

Slc16a8 solute carrier family 16 (monocarboxylic acid transporters), member 8 ( MGI:1929519)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004301. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004301_01	euxassay_004301_02	euxassay_004301_03	euxassay_004301_04

euxassay_004301_05	euxassay_004301_06	euxassay_004301_07	euxassay_004301_08	euxassay_004301_09

euxassay_004301_10	euxassay_004301_11	euxassay_004301_12	euxassay_004301_13	euxassay_004301_14

euxassay_004301_15	euxassay_004301_16	euxassay_004301_17	euxassay_004301_18	euxassay_004301_19

euxassay_004301_20	euxassay_004301_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14461_wholemount_strong.wlz

14461_wholemount_moderate.wlz

14461_wholemount_weak.wlz

14461_wholemount_possible.wlz

14461_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14461_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4136
Entity Detected:	Slc16a8, solute carrier family 16 (monocarboxylic acid transporters), member 8 ( MGI:1929519)
Sequence:	sense strand is shown >T4136 GTGCAGCCACCATTGCCAAGGCTGTTGGACACTCTCTGGAGCTAGAAGCTGCCCCTCCCGCAGGCCTCGA GAGATGTGAGGCTCAACCCTAAATCCAGAGTCAGGTCCAGGTTCCCAGAGGAGAGGAGCCCTGGTGAGGA AACTCCGAAGTGAAGTTTCCATTCACCCTTCACAGGCCTTTGCAGGAAGAAGCAGAAGCAATGGGTGCTG GCGGTCCGAGGCGGGGTGCTGGCCCTCCGGATGGTGGCTGGGGCTGGGTGGTGCTGGGCGCCTGCTTCGT GGTCACCGGCTTCGCCTATGGCTTCCCTAAGGCTGTGAGCGTCTTCTTCCGAGAGCTCAAGCGCGACTTT GGGGCAGGTTACAGTGACACAGCCTGGGTGTCCTCCATCATGTTGGCCATGCTCTACGGCACGGGCCCCT TGTCCAGCATCCTCGTGACGCGCTTTGGCTGTCGCCCCGTGATGCTGGCTGGTGGACTGCTGGCTTCAGC AGGCATGATTCTGGCTTCCTTTGCCTCGCGCCTCGTGGAGCTGTACCTGACGGCTGGGGTGCTCACAGGC CTGGGCCTAGCTCTCAACTTCCAGCCGTCGCTCATCATGCTGGGACTCTACTTCGAGCGGCGACGGCCCC TGGCCAACGGGCTGGCAGCGGCGGGCAGTCCCGTGTTTCTGTCCATGCTGTCGCCGCTCGGGCAGCTGCT GGGGGAGCGCTTCGGCTGGCGTGGCGGCTTCCTGCTGTTTGGCGGCCTCCTGCTGCACTGTTGCGCCTGT GGGGCAGTCATGCGTCCGCCCCCTGGACCCCCGCCGCGCAGAGACCCCTCGCCCCACGGTGGCCCGGCTC GCCGCCGGCGGCTGCTGGACGTGGCAGTGTGCACCGACCGTGCTTTCGTGGTGTACGTGGTCACCAAGTT CCTGATGGCACTCGGGCTCTTTGTGCCGGCCATCCTGCTGGTGAACTACGCCAAGGACGCGGGCGTGCCT GACGCCGAGGCCGCCTTCTTGCTGTCCATCGTGGGCTTCGTGGACATCGTGGCACGACCGGCGTGCGGTG CTTTGGCGGGTCTGGGGCGCCTGAGACCCCACGTCCCCTACCTCTTCAGCCTGGCCCTGTTGGCCAATGG GCTCACGGACCTGATTAGCGCGCGTGCGCGCTCCTACGGGACCCTCGTTGCTTTCTGCATTGCCTTCGGC CTGTCCTACGGCATGGTGGGCGCACTGCAGTTTGAGGTGCTCATGGCAACCGTGGGTGCACCCCGCTTCC CTAGTGCATTGGGCCTAGTGCTGCTCGTGGAGGCCGTGGCTGTGCTCATAGGACCGCCCTCTGCCGGCCG CCTGGTGGATGCTCTGAAGAACTATGAAATCATCTTCTACCTTGCTGGCTCTGAAGTGGCCTTGGCAGGA GTCTTCATGGCAGTTACCACCTACTGCTGCCTGCGCTGCTCTAAGAACATCTCATCAGGTCGATCTGCTG AGGGAGGAGCCAGCGACCCAGAGGATGTGGAGGCTGAGAGGGACTCTGAGCCAATGCCTGCCAGCACTGA GGAGCCGGGCAGCCTTGAAGCTCTGGAGGTGCTGAGCCCCCGGGCTGGGTCCCCAGAACAAGAACCAGAG GAGGAGGCAGTGCCTGAGCTGGATCACGAGTCCATAGGTGGCCATGAGGCACGAGGCCAGAAGGCATGAA ACTGTGGCTTCCTCTCTGAGGGCCTGGTACATGGGGAGCCAGCCTGGGGTGGTCTCTTGGGAACCCAATA TGTTAGACCCTGTTCACAGTGAATAAAGGGCCCCTAAAAGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4481698 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4481698 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14464 same embryo
	EMAGE:14460 same embryo
	EMAGE:14463 same embryo
	EMAGE:14462 same embryo
	EurExpress:euxassay_004301 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source