EMAGE mouse gene expression spatial database

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EMAGE:14463

Tarsl2 threonyl-tRNA synthetase-like 2 ( MGI:2444486)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_004302. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_004302_01	euxassay_004302_02	euxassay_004302_03	euxassay_004302_04

euxassay_004302_05	euxassay_004302_06	euxassay_004302_07	euxassay_004302_08	euxassay_004302_09

euxassay_004302_10	euxassay_004302_11	euxassay_004302_12	euxassay_004302_13	euxassay_004302_14

euxassay_004302_15	euxassay_004302_16	euxassay_004302_17	euxassay_004302_18	euxassay_004302_19

euxassay_004302_20	euxassay_004302_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14463_wholemount_strong.wlz

14463_wholemount_moderate.wlz

14463_wholemount_weak.wlz

14463_wholemount_possible.wlz

14463_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14463_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

thalamus mantle layer

moderate

regional

moderate expression: see section 14 15

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4098
Entity Detected:	Tarsl2, threonyl-tRNA synthetase-like 2 ( MGI:2444486)
Sequence:	sense strand is shown >T4098 TGGCGGGCTCAGGTGGCACAGGGAAGCTACCATGGCGGCCCAGGCCCTGGCGGCTCAGGCGGTGGCCTCG CGCCTGCAGCGGCAGGAGGAGGACATCCGCTGGCTGTGCGCGGAGGTGCAGCGCCTGAGGGACGAGCAGC TGCGCGGGCCCGAGCGGGGCCAAGCCGAGGGCCCGCGCCTGACACGCGAGGTGGCACAGCTCCAGGCGGA GAACCGCGACCTGCACCAGCGCCTGTGTGGCCTGCGGCTGCGCCTGGCGGAGCAGCGCCGCACCGAGGCC GGGCGAGCGGCGGCGCACGAGCCTCCTACCCAAAACCAAGAGAAGGACACAAAGAAGAAGAGGCTGAAGC AGAGTGAGCCTGGCCGGGAGGTGAAGCAACCGAATTTTATAAAAGAGAGATTGCAGCTTTTTGAAACATT GAAGACAGATCATCAACTGTTACCTGCTACTCAGGAAAAGAAGAACACAAACAACGTGATTTCAGTGAGG GTGGCTGGTGGGAAGACAGTGCAAGGAGAAAGGTGGAAAACAACACCTTATCAAGTGGCCGCTGGAATTA GTAAGGAATTGGCTGAACATACAGTGATTGCCAAAGTGAACGGTGTATTGTGGGATCTGGACCGCCCGTT GGAAGGGGACTCCACTGTGGAGCTGCTGATGTTTGACAACGAGGAAGCCCAGGCTGTGTACTGGCATTCC AGCGCTCACATCCTGGGGGAGGCCATGGAACTTTACTATGGAGGCCACCTGTGTTATGGCCCACCCATTG AAAATGGGTTTTATTATGATATGTTTATTGAAGACAGAGTGGTGTCCAGCACTGAGTTGTCTGCCCTGGA GAACATATGTAAAACTATCATCAAAGAAAAGCAACCGTTTGAAAGACTGGAAGTCAGCAAGGACACTCTC CTGGAAATGTTTAAGTACAATAAATTTAAGTGCCGGATTCTGAAGGAGAAAGTGGACACTCCGACGACCA CTGTTTACAGGTGTGGTCCATTGATTGACCTTTGTAAAGGCCCCCATGTAAGACACACTGGAAAAATTAA AGCCATAAAAATTTTCAAGAACTCCTCAACATATTGGGAAGGCAACCCTGAGATGGAAACTTTGCAGCGG ATCTATGGGATATCCTTTCCTGACAGCAAAATGATGAAAGACTGGGAAAAAATCCAAGAGGAAGCCAAGA GCCGGGACCACAGGAAAATCGGGAAGGAACAAGAACTTTTCTTCTTCCATGATCTGAGCCCTGGAAGCTG TTTTTTCCTCCCGAGAGGAGCGTTCATTTACAATGCGCTTATGGATTTCATACGAGAGGAATATCACAAG CGTAACTTCACAGAAGTGCTCTCTCCCAACATGTACAACAGTAAACTCTGGGAAACATCAGGCCACTGGC AGCATTACAGTAACAACATGTTCACCTTTGACGTCGAGAAGGACACTTTCGCCCTCAAACCCATGAACTG TCCAGGGCACTGTTTAATGTTCGCACATCGCCCGCGATCCTGGAGGGAAATGCCTGTTAGATTTGCTGAT TTTGGAGTTCTTCACAGAAATGAGCTGTCAGGCACTTTAAGCGGCTTAACCCGAGTGAGGCGCTTCCAGC AAGATGATGCGCACATCTTCTGCATGGTGGAGCAGATCGAAGAAGAAATAAAGGGGTGCTTGCACTTTTT ACAGTCCGTTTACTCGACGTTTGGCTTCTCCTTTCAATTAAACCTGTCCACAAGGCCTGAGCACTTCCTA GGAGAGATTGAGATATGGGATGAAGCTGAAAGGCAACTGCAGAACAGCTTGGTGGAATTTGGAAAACCAT GGAAAATAAACCCAGGAGATGGAGCATTCTATGGCCCTAAAATCGATATAAAAATTAAAGATGCTATTGG CAGATACCATCAGTGTGCTACAATTCAGCTGGACTTCCAACTGCCTATCAGGTTTAACCTCACATATGTG AGTAAGGATGGGGATGACAAGAACAGGCCTGTGATCATTCACCGCGCCATCCTGGGGTCTGTGGAAAGGA TGATAGCCATCCTCTCAGAGAACTACGGGGGGAAATGGCCTCTCTGGCTGTCCCCTCGCCAGGTAATGGT CATCCCAGTAGGCCCAGCTTGTGAAAATTATGCACTACAGGTGTCCAAGGAATGCTTCGAAGAAGGCTTC ATGGCCGATGTGGACTTAGATGACAGTTGTACACTAAATAAGAAAATACGGAATGCACAGCTGGCTCAGT ATAATTTCATTTTGGTGGTTGGCGAAAAGGAAAAGATCAACAATGCAGTAAATGTTCGAACAAGAGACAA CAAAATTCATGGAGAAATATCAATAGCTTCCGTCATTGAAAAATTGAAGAACCTCAAAAAATCACGTACG CTAAATGCCGAGGAAGACTTCTGAAGGCTTCTCCTTGGGCTTCTATATAACCTTGTTTTGACTTCTGAAA TGTATTTTCCCTGTCACTTAAGATATTAGTACTTCTAAGAACCCTAGGGCCACTTGTAGGGCTCTAATGA GTTCAACCTAAGAAGGAAAAGTGATTATTTGATATAAACAAAATTTTAATTTGTTGTGTTCTGAGACTAA ATAGGAGAAGCTGGAGAAATTTGCAAGCTATCAAGACTCCAGAGACCTCAGTGATAAAATGGTGTCCATT CAAAGAGGGGAACACTAGCGAGAATTATCAGAAATCATGCTTTGTTTCTTTCTCAAAAGATGTGCTTCCA GCCTGGGGGCTGTAGCTTCGTGACCAGAGCATCCGCGTTGCATGTATGAGGCCTGAGCTCCATCCCCAGC AGCCACCACCTCTCACCCAACAATGACAGGAATAGGCTTTCAATAAAAGCCCATGGCCCTTTTGTAACAT GGAGGGGAGATGTCTTATTTTCTAATACACACATCTCAGGTATTTTTATGGCTGCTTTACTAAAGTTCAC ACTGAAACTCATGCCCAAAGCTGGAATTGCTATTTCATTTAGTGTGCAATCATAAGAGAAGTACTAATAT CCTGTTAGTGGCAAAGGACTATGAAACCTCAAAGAGATTTTGTACAATCTGTTATTTGCATTGTGAGTTA AGAGTTACAGTTTTAATGCTTCTAATCATAATTCATTATATTTATCTTTAGAAACACTAAAGTCCTAAAT AAAATGCATCACATGGTGTTTCTAGAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4458085 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4458085 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14464 same embryo
	EMAGE:14460 same embryo
	EMAGE:14461 same embryo
	EMAGE:14462 same embryo
	EurExpress:euxassay_004302 same experiment
	MGI:4828602 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source