EMAGE mouse gene expression spatial database

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EMAGE:14492

Aifm2 apoptosis-inducing factor, mitochondrion-associated 2 ( MGI:1918611)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005341. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005341_01	euxassay_005341_02	euxassay_005341_03	euxassay_005341_04

euxassay_005341_05	euxassay_005341_06	euxassay_005341_07	euxassay_005341_08	euxassay_005341_09

euxassay_005341_10	euxassay_005341_11	euxassay_005341_12	euxassay_005341_13	euxassay_005341_14

euxassay_005341_15	euxassay_005341_16	euxassay_005341_17	euxassay_005341_18	euxassay_005341_19

euxassay_005341_20	euxassay_005341_21	euxassay_005341_22	euxassay_005341_23	euxassay_005341_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14492_wholemount_strong.wlz

14492_wholemount_moderate.wlz

14492_wholemount_weak.wlz

14492_wholemount_possible.wlz

14492_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14492_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

strong

regional

strong expression: see section 07 08 09 moderate expression: see section 15 16 weak expression: see section 06 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8283
Entity Detected:	Aifm2, apoptosis-inducing factor, mitochondrion-associated 2 ( MGI:1918611)
Sequence:	sense strand is shown >T8283 CACGGCCTAACTTTTCTTGGCATTTGACTCTGAAGTCCTCAAGACCGAGAAGAGCGGTTTGGCTTTGGTG GGGGCTGGCTCAGCTCCCAAGTGCCAAGTTCAAGATGGGGTCCCAGGTCTCGGTGGATACAGGAGCCGTG CACGTGGTGATCGTGGGCGGGGGCTTCGGAGGGATAGCGGCTGCCAGCCAGCTGCAGGCGCTGAATGTCC CCTTCATGCTGGTGGATATGAAGGACTCCTTCCACCACAATGTGGCAGCCCTCCGGGCCTCCGTGGAGAG CGGGTTCGCCAAAAAGACATTCATTTCGTACTCTGCGACCTTCAAGGACAACTTCCGCCAGGGCAAAGTG ATTGGCATAGACTTGAAGAACCGGATGGTGTTGCTACAGGGTGGCGAGGCCTTGCCCTTCTCACATCTTA TCCTGGCCACAGGCAGCACCGGACCCTTCCCTGGCAAGTTTAACGAGGTGTCCTGCCAGCAGGCAGCCAT CCAGGCCTATGAGGACATGGTGAAGCAGATCCAGCGCTCACAATTCATCGTGGTGGTGGGAGGCGGCTCT GCAGGAGTAGAGATGGCAGCAGAGATTAAAACCGAGTACCCTGAGAAGGAGGTCACTCTTATCCACTCCA GAGTACCCCTGGCCGACAAGGAACTCCTGCCCTGTGTGCGGCAGGAAGTGAAGGAGATCCTCCTCCGGAA GGGTGTGCAGCTGCTGCTGAGTGAGCGGGTGAGCAACCTGGAGGAACTGCCTCGCAATGAGTATCGGGAG TACATCAAGGTGGAGACAGACAAGGGCACGGAGGTGGCCACCAACATGGTGATTGTGTGCAATGGGATCA AGATCAACAGCTCTGCCTACCGCAGTGCATTTGAGAGTAGGCTGGCTAGCAATGGTGCTCTGAAAGTGAA CGAGTTCCTCCAGGTGGAAGGTTACAGCAATATTTATGCCATTGGTGACTGTGCCGATACCAAGGAGCCC AAGATGGCCTACCACGCTGGCCTGCATGCCAATGTTGCCGTGGCCAACATCGTCAACTCCATGAAGCAGA GGCCACTCAAAGCTTACAAGCCAGAGACTGACCAACCACCGGCAGCTTTGTCCCCGGCTCTGCTGCTGTG AACACCAGCCAGGAAGCTCACCCTCTCTGAGGGAAGGATCAACTACCTGCAGGAAGCCGGCCATGTGAGA ACTCTACAGAGCATGGCGGGACTCTTCAGTGACAGGCCTTGTGTTATTTCTTTATAACCGTCTGGATGAA TCTGTCTGTGGGGAACGCGGTTGTCCAGAAGGAGGAAAATATCGCAATAAAGGGCTATGTTATAAGAAAA AAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1395873 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1395873 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14496 same embryo
	EMAGE:14493 same embryo
	EMAGE:14491 same embryo
	EMAGE:14495 same embryo
	EMAGE:14494 same embryo
	EurExpress:euxassay_005341 same experiment
	MGI:4823051 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source