Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14493

Sarnp SAP domain containing ribonucleoprotein ( MGI:1913368)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14493 EMAGE:14493 EMAGE:14493 EMAGE:14493 EMAGE:14493
"Pseudo-wholemount" of euxassay_005338. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005338_01 euxassay_005338_02 euxassay_005338_03 euxassay_005338_04
EMAGE:14493 EMAGE:14493 EMAGE:14493 EMAGE:14493 EMAGE:14493
euxassay_005338_05 euxassay_005338_06 euxassay_005338_07 euxassay_005338_08 euxassay_005338_09
EMAGE:14493 EMAGE:14493 EMAGE:14493 EMAGE:14493 EMAGE:14493
euxassay_005338_10 euxassay_005338_11 euxassay_005338_12 euxassay_005338_13 euxassay_005338_14
EMAGE:14493 EMAGE:14493 EMAGE:14493 EMAGE:14493 EMAGE:14493
euxassay_005338_15 euxassay_005338_16 euxassay_005338_17 euxassay_005338_18 euxassay_005338_19
EMAGE:14493 EMAGE:14493 EMAGE:14493 EMAGE:14493
euxassay_005338_20 euxassay_005338_21 euxassay_005338_22 euxassay_005338_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14493Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14493_wholemount_strong.wlz
14493_wholemount_moderate.wlz
14493_wholemount_weak.wlz
14493_wholemount_possible.wlz
14493_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14493_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8277
Entity Detected:Sarnp, SAP domain containing ribonucleoprotein ( MGI:1913368)
Sequence:sense strand is shown

>T8277
CAAGATGGCGGCCGAGACGGTGGAGCTCCACAAGCTGAAGCTTGCTGAACTAAAGCAAGAATGTCTTGCT
CGTGGTTTAGAGACCAAGGGAATAAAACAAGATCTTATAAATAGGCTGCAGGCCTATCTTGAAGACCATG
CAGAAGAAGAAGCAAATGAAGAAGATGTACTGGGAGATGAAACTGAGGAAGAAGAACCAAAGCCCATAGA
ACTGCCTGTTAAAGAGGAAGAACCTCCTGAAAAGGCTGTTGATATGGCATCGGAAAAGAAGGTGGTAAAG
ATTACATCTGGGATACCTCAGACTGAAAGAATGCAGAAGAGGGCTGAACGATTCAATGTACCTGTAAGCC
TGGAGAGTAAGAAGGCTGCCCGGGCGGCTAGGTTTGGAATTTCTTCAGTTCCAACAAAAGGTTTATCATC
TGACACCAAGCCTATGGTTAACCTGGATAAACTAAAGGAAAGAGCACAGAGATTTGGTTTGAATGTCTCT
TCCATCTCTAGAAAGTCTGAGGATGATGAGAAGCTGAAGAAACGGAAGGAGAGATTTGGGATTGTGACAA
GTTCAGCTGGAACTGGAACCACAGAGGATACAGAGGCAAAGAAAAGAAAAAGAGCAGAGCGTTTTGGAAT
TGCATAATGAAAAGTTCGTGCTTTCTGCACCATAGTGTTTTCCATTTCTCAAATTTTTCTTGGTTTTATA
TATACATACACACCCCTACCTACCTACCTATCTACATACACAGTCATGTGCCTCACAGTGAGGAAGCATG
TACGTTTGGTACATCCTTGAATTCAGTAGCAGTTTTATTTAACTGCTCTTCTGCGTTTAAGATTGTTGTG
GTTTTTGTTTTTAATTATTTTTGCTTATTAATAAAAAAGGAAGAAAAGAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:1382354 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1382354 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14496 same embryo
 EMAGE:14492 same embryo
 EMAGE:14491 same embryo
 EMAGE:14495 same embryo
 EMAGE:14494 same embryo
 EurExpress:euxassay_005338 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS