EMAGE mouse gene expression spatial database

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EMAGE:14509

Sncg synuclein, gamma ( MGI:1298397)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005364. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005364_01	euxassay_005364_02	euxassay_005364_03	euxassay_005364_04

euxassay_005364_05	euxassay_005364_06	euxassay_005364_07	euxassay_005364_08	euxassay_005364_09

euxassay_005364_10	euxassay_005364_11	euxassay_005364_12	euxassay_005364_13	euxassay_005364_14

euxassay_005364_15	euxassay_005364_16	euxassay_005364_17	euxassay_005364_18	euxassay_005364_19

euxassay_005364_20	euxassay_005364_21	euxassay_005364_22	euxassay_005364_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14509_wholemount_strong.wlz

14509_wholemount_moderate.wlz

14509_wholemount_weak.wlz

14509_wholemount_possible.wlz

14509_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14509_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

diencephalon lateral wall mantle layer

strong

regional

strong expression: see section 06 07 08 09 12 13

medulla oblongata basal plate mantle layer

strong

regional

strong expression: see section 04 05 06 07 08 12 13 moderate expression: see section 09 10 11 not examined expression: see section 14 15

pons mantle layer

strong

regional

strong expression: see section 04 05 06 12 moderate expression: see section 03 13 14 15

midbrain mantle layer

strong

regional

strong expression: see section 02 03 04 05 06 07 08 09 10 11 12 13

facial vii ganglion

strong

regional

strong expression: see section 02 03 04 19

glossopharyngeal ix ganglion

strong

regional

strong expression: see section 03 04 14 15

trigeminal v ganglion

strong

regional

strong expression: see section 02 03 04 05 06 07 08 15 16 17 18 19 20

vagus x ganglion

strong

regional

strong expression: see section 05 14 weak expression: see section 06

vestibulocochlear viii ganglion

strong

regional

strong expression: see section 03 04 05 15 16

ventral grey horn

strong

regional

strong expression: see section 04 05 06 07 08 09 10 11

dorsal root ganglion

strong

regional

strong expression: see section 03 04 05 06 07 08 09 10 11 12 13

neural retina

strong

regional

strong expression: see section 01 02 03 04 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8309
Entity Detected:	Sncg, synuclein, gamma ( MGI:1298397)
Sequence:	sense strand is shown >T8309 GCAGTAGCAGCAGAGACAGCGGCTGCGGCAGCACTCCAGTCCATAGCTTGCAGCAGCCAGGTTCCATCCT TGCAAACACCATGGACGTCTTCAAGAAAGGCTTCTCCATTGCCAAGGAAGGTGTTGTGGGTGCTGTAGAA AAGACCAAGCAGGGAGTAACGGAGGCAGCTGAGAAGACCAAGGAGGGGGTTATGTATGTGGGCACCAAAA CCAAGGAGAACGTGGTACAAAGTGTCACCTCAGTGGCTGAGAAGACCAAGGAGCAGGCCAATGCCGTGAG TGAAGCTGTGGTCAGCAGCGTCAACACAGTGGCCAACAAGACCGTGGAGGAGGCTGAAAACATCGTGGTC ACCACCGGGGTGGTGCGCAAGGAGGACTTGGAACCCCCTGCACAGGACCAAGAAGCCAAAGAGCAAGAGG AGAATGAAGAGGCCAAGAGTGGAGAAGACTAGAAGGCTGCCAGCCAACCACAGAGGCTCAAGGGACACCC CTCTGCCTTGGACCCTGTCTCAACCTGGCACACTGAATGCCCTGCCTAGAGTGCCCTGCAGGTTGCCCAA GTTCTCTGTCCCTAGCCCAGTGCCACAAGTCCACACACGCTACTGCCAGCGCCAGCACCCCCTGCTGGCT CCTTTTACCCTATGGTCCTCTGATCACCAATCAGATGCTGCTGTGATTTCTTTTTTTTTAATGATTCCAA ATAAAACCTGAGCCCCACTCCCAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1448798 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1448798 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14510 same embryo
	EurExpress:euxassay_005364 same experiment
	MGI:4828346 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source