EMAGE mouse gene expression spatial database

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EMAGE:14523

Trappc2 trafficking protein particle complex 2 ( MGI:1913476)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005400. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005400_01	euxassay_005400_02	euxassay_005400_03	euxassay_005400_04

euxassay_005400_05	euxassay_005400_06	euxassay_005400_07	euxassay_005400_08	euxassay_005400_09

euxassay_005400_10	euxassay_005400_11	euxassay_005400_12	euxassay_005400_13	euxassay_005400_14

euxassay_005400_15	euxassay_005400_16	euxassay_005400_17	euxassay_005400_18	euxassay_005400_19

euxassay_005400_20	euxassay_005400_21	euxassay_005400_22	euxassay_005400_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14523_wholemount_strong.wlz

14523_wholemount_moderate.wlz

14523_wholemount_weak.wlz

14523_wholemount_possible.wlz

14523_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14523_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 05

trigeminal v ganglion

moderate

regional

moderate expression: see section 03 04 05 06 07 08 16 17 18 19 20 weak expression: see section 02

vagus x ganglion

moderate

regional

moderate expression: see section 06 07 weak expression: see section 16

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 05 06 weak expression: see section 16 17

ventral grey horn

moderate

regional

moderate expression: see section 07 08 09 weak expression: see section 10 11 12

dorsal root ganglion

moderate

regional

moderate expression: see section 12 13 14 15 weak expression: see section 05 06 07 11

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8339
Entity Detected:	Trappc2, trafficking protein particle complex 2 ( MGI:1913476)
Sequence:	sense strand is shown >T8339 GAGCGCAGATTAAAGAGGCGGCCGGTTCTCATCTCGGCTCCTCCCTACAGGAGCTGTGTATTGAAGACAA TGTCTGGGAGCTTCTACTTCGTAATTGTTGGCCACCATGATAATCCAGTTTTTGAAATGGAATTTTTGCC ACCTGGGAAAGCAGAATCCAAAGATGATCACCGTCATCTGAACCAGTTTATAGCTCATGCTGCTCTTGAC CTTGTGGACGAAAATATGTGGCTTTCGAACAACATGTACTTAAAAACGGTGGACAAATTTAATGAGTGGT TTGTATCAGCCTTTGTCACTGCAGGGCATATGAGATTTATTATGCTTCATGATGTGAGGCAAGAAGATGG GATAAAGAACTTCTTTACTGATGTCTATGATCTGTATATAAAGTTTGCAATGAATCCATTTTATGAACCC AATTCTCCCATTCGATCGAGTGCATTCGACAGGAAAGTCCAGTTTCTTGGGAAGAAACACCTTTTAAGCT AAATGAGAACAACTCGGAAATCAGCTGTCTCATCGTGGGTATCAGGAAGGTGTGCTCTGTAAATCATTCG AAATTGCCTGGAAAACATCCCCTTAAAATTGTACTTTGTTTTCATGTTGACTATCAGCATTAATATTGGC TTGTGAACTGTGATATGTATAAACAACTCTTTACCAGTAGTTCTGAACAGTTTTATTTAAAAACTTGAGA AAACTTTTCTTGCCGAAGATAATATGATCTTAATTTATTAATTCAAAAGCATATGTCATAGTTAATATTT GTTAGATTGATTAAAGACTGAAAAGTTCTGGAGACATGACTGAAATGAGTTATTTATACATTTAGAGATG TCCTTTTAAAATGCATAATACACTGCAGTACCAAGAAGCTGAAGCAGAAAGCCCATGCCAAGTGTTCCAT GCTTGCCTACCTCTGCCAGAGGCTGAGAATAACCGTCCTGCTGTAACTCTAGACCCCCTAGATCTATGTA TGCTGCACCAGTTACAGGCCTTGGCAAAATGTGGTGCCTCTGTCACCTTACATTCTAGTGTTTCCTTTCT CTTAGTAAACTAGAGGTTTATTTGAATTTATTAGTATCTGTACTTGGAAAATGTTATAATATGGATTTAG TATTACTAATAAAAGCCTGGGTTACACTGACATTTGCACTTTACCTTTATGCTATATACAGGGTGGGAGG TGGCAGATGTGGCCTCTTGTATTAGAAATGTTGGTTTGATTTTACAGTAGTACATATGTATGGAAAAGCA AACCAGATTTTCATGTACACACTGGAAAATTTCTCTCCATTCTAACTTTTGAGTAAGTACTTCTAAACTT TCTTGTATTCTTTGGTGAAGTTATCATCACTCTTTTTTTTATTTCTTAAATAAATATTTATATCCAAAAA AAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:1528500 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:1528500 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14526 same embryo
	EMAGE:14524 same embryo
	EMAGE:14525 same embryo
	EMAGE:14527 same embryo
	EurExpress:euxassay_005400 same experiment
	MGI:4828896 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source