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EMAGE:14532

Tmem100 transmembrane protein 100 ( MGI:1915138)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14532 EMAGE:14532 EMAGE:14532 EMAGE:14532 EMAGE:14532
"Pseudo-wholemount" of euxassay_005383. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005383_01 euxassay_005383_02 euxassay_005383_03 euxassay_005383_04
EMAGE:14532 EMAGE:14532 EMAGE:14532 EMAGE:14532 EMAGE:14532
euxassay_005383_05 euxassay_005383_06 euxassay_005383_07 euxassay_005383_08 euxassay_005383_09
EMAGE:14532 EMAGE:14532 EMAGE:14532 EMAGE:14532 EMAGE:14532
euxassay_005383_10 euxassay_005383_11 euxassay_005383_12 euxassay_005383_13 euxassay_005383_14
EMAGE:14532 EMAGE:14532 EMAGE:14532 EMAGE:14532 EMAGE:14532
euxassay_005383_15 euxassay_005383_16 euxassay_005383_17 euxassay_005383_18 euxassay_005383_19
EMAGE:14532 EMAGE:14532 EMAGE:14532 EMAGE:14532
euxassay_005383_20 euxassay_005383_21 euxassay_005383_22 euxassay_005383_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14532Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14532_wholemount_strong.wlz
14532_wholemount_moderate.wlz
14532_wholemount_weak.wlz
14532_wholemount_possible.wlz
14532_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14532_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
head mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 13 14 15 16 17 18 19
trunk mesenchyme
moderate moderate
regionalmoderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19
spinal cord ventricular layer
moderate moderate
regionalmoderate expression: see section 09 10 11
cornea
strong strong
regionalstrong expression: see section 01 02 03 moderate expression: see section 04 not examined expression: see section 23
lower eyelid
strong strong
regionalstrong expression: see section 02 03 moderate expression: see section 23
upper eyelid
strong strong
regionalstrong expression: see section 02 03 moderate expression: see section 23
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T8348
Entity Detected:Tmem100, transmembrane protein 100 ( MGI:1915138)
Sequence:sense strand is shown

>T8348
GGCTGAGAAAGAGGCAATCCCTGGTCTGTCCTTTTACCAATGCCCAGTGGGTGACAGGCTCTTTCCCAGA
AGTTGAACGGAGCTACGCTGGCAGATCCCTCTCTCCCAAGTCAAGTGGCCTCTCTGGTAATGGACTGCCT
TTCTGTGAGCTTGCATCCTGACCAGGCTTTCCAAATCTAGCCTGTGAAGCGAGATAAGAAAAATCCCACA
GAAGAAAAAAAAATGACCGAAGAATCCACAAAAGAGAACCTGGGAGCTCCAAAATCTCCCACACCTGTGA
CAATGGAGAAAAACCCCAAGAGGGAAGTTGTGGTCACCACGGGACCCTTGGTCAGCGAGGTTCAGCTGAT
GGCCGCCACCGGGGGTGCCGAACTCTCCTGCTACCGCTGCATCATCCCCTTTGCCGTGGTGGTCTTCATC
ACTGGGATTGTGGTCACCGCTGTGGCTTACAGCTTCAATTCCCATGGTTCCATCATCTCCATCTTCGGCC
TGGTCCTTCTGTCCTCCGGACTGTTTTTACTAGCCTCCAGTGCCTTGTGCTGGAAGGTGAGACAAAGGAA
CAAGAAAGTCAAGAGACGCGAGAGTCAGACCGCTCTTGTGGTAAATCAGAGGTGCTTGTTTGCTTAAGAC
TGAGTAAGAGCAAACGCGAACGCTCACCCGCCCACACTGCTCTAACTCAGTGAACTCATTCATAGGAAAC
CAGCCCGGTACCGGATGAGCTCAACTTTCGAATGAACTACCAAAATGAAAAATAGGGCATAGAGAGGACT
GAAAATGAGGGGTCAAGACGGTTCCCCCTTGGTAGGGAATAACTCATCTTTAACGACAAACTTAACCCTA
AGGGCTATTTCTAACACAGACAGAGGAATCAGCTTGCTTTTCCTGTTAAACGTTTAGGGAGTGTGGGAGA
TGCACAGCATTAAATAACAGTTGGTTCCATTTAGAAAGTACCCAAGGGAAGAATGGACAAATAATGAGAG
CCCTGGCAAGTGGTGTTATAAAAACGTTCCACCAAAAGCCTACATTGGCTTGGCATTCCCACGTACCTAA
GAAGTTCTGTTATATATATACATATATATTTTTTCCAATAAGTTGATTCTTTGCCCCCCCCTTTTTAAAA
GAATTTTCACTTTCAGTAACATCACTAGAGGTACTTTATTTTGAAGAATAGACTAATATTTTTTATATTT
TAACAATGGACAATTGTAGATGGTTGTAATGATATGTCAGAAGAAAACAGAAATGTAGGTAACACAGATG
ACACAGGGACAGTTAAATTAATATTGAAATAATCCAATCTAGCACCTTTGATGGCTTTTATACAAAAGTT
CAGTGTGCATTTCACTCAAAATAATAAATGCTCATGGCTGCTGAAACTTAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:3326079 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3326079 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14528 same embryo
 EMAGE:14529 same embryo
 EMAGE:14531 same embryo
 EMAGE:14530 same embryo
 EurExpress:euxassay_005383 same experiment
 MGI:4828762 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS