EMAGE mouse gene expression spatial database

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EMAGE:14532

Tmem100 transmembrane protein 100 ( MGI:1915138)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005383. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005383_01	euxassay_005383_02	euxassay_005383_03	euxassay_005383_04

euxassay_005383_05	euxassay_005383_06	euxassay_005383_07	euxassay_005383_08	euxassay_005383_09

euxassay_005383_10	euxassay_005383_11	euxassay_005383_12	euxassay_005383_13	euxassay_005383_14

euxassay_005383_15	euxassay_005383_16	euxassay_005383_17	euxassay_005383_18	euxassay_005383_19

euxassay_005383_20	euxassay_005383_21	euxassay_005383_22	euxassay_005383_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14532_wholemount_strong.wlz

14532_wholemount_moderate.wlz

14532_wholemount_weak.wlz

14532_wholemount_possible.wlz

14532_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14532_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

head mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 13 14 15 16 17 18 19

trunk mesenchyme

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

spinal cord ventricular layer

moderate

regional

moderate expression: see section 09 10 11

cornea

strong

regional

strong expression: see section 01 02 03 moderate expression: see section 04 not examined expression: see section 23

lower eyelid

strong

regional

strong expression: see section 02 03 moderate expression: see section 23

upper eyelid

strong

regional

strong expression: see section 02 03 moderate expression: see section 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8348
Entity Detected:	Tmem100, transmembrane protein 100 ( MGI:1915138)
Sequence:	sense strand is shown >T8348 GGCTGAGAAAGAGGCAATCCCTGGTCTGTCCTTTTACCAATGCCCAGTGGGTGACAGGCTCTTTCCCAGA AGTTGAACGGAGCTACGCTGGCAGATCCCTCTCTCCCAAGTCAAGTGGCCTCTCTGGTAATGGACTGCCT TTCTGTGAGCTTGCATCCTGACCAGGCTTTCCAAATCTAGCCTGTGAAGCGAGATAAGAAAAATCCCACA GAAGAAAAAAAAATGACCGAAGAATCCACAAAAGAGAACCTGGGAGCTCCAAAATCTCCCACACCTGTGA CAATGGAGAAAAACCCCAAGAGGGAAGTTGTGGTCACCACGGGACCCTTGGTCAGCGAGGTTCAGCTGAT GGCCGCCACCGGGGGTGCCGAACTCTCCTGCTACCGCTGCATCATCCCCTTTGCCGTGGTGGTCTTCATC ACTGGGATTGTGGTCACCGCTGTGGCTTACAGCTTCAATTCCCATGGTTCCATCATCTCCATCTTCGGCC TGGTCCTTCTGTCCTCCGGACTGTTTTTACTAGCCTCCAGTGCCTTGTGCTGGAAGGTGAGACAAAGGAA CAAGAAAGTCAAGAGACGCGAGAGTCAGACCGCTCTTGTGGTAAATCAGAGGTGCTTGTTTGCTTAAGAC TGAGTAAGAGCAAACGCGAACGCTCACCCGCCCACACTGCTCTAACTCAGTGAACTCATTCATAGGAAAC CAGCCCGGTACCGGATGAGCTCAACTTTCGAATGAACTACCAAAATGAAAAATAGGGCATAGAGAGGACT GAAAATGAGGGGTCAAGACGGTTCCCCCTTGGTAGGGAATAACTCATCTTTAACGACAAACTTAACCCTA AGGGCTATTTCTAACACAGACAGAGGAATCAGCTTGCTTTTCCTGTTAAACGTTTAGGGAGTGTGGGAGA TGCACAGCATTAAATAACAGTTGGTTCCATTTAGAAAGTACCCAAGGGAAGAATGGACAAATAATGAGAG CCCTGGCAAGTGGTGTTATAAAAACGTTCCACCAAAAGCCTACATTGGCTTGGCATTCCCACGTACCTAA GAAGTTCTGTTATATATATACATATATATTTTTTCCAATAAGTTGATTCTTTGCCCCCCCCTTTTTAAAA GAATTTTCACTTTCAGTAACATCACTAGAGGTACTTTATTTTGAAGAATAGACTAATATTTTTTATATTT TAACAATGGACAATTGTAGATGGTTGTAATGATATGTCAGAAGAAAACAGAAATGTAGGTAACACAGATG ACACAGGGACAGTTAAATTAATATTGAAATAATCCAATCTAGCACCTTTGATGGCTTTTATACAAAAGTT CAGTGTGCATTTCACTCAAAATAATAAATGCTCATGGCTGCTGAAACTTAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3326079 using vector (pT7T3D-PacI) specific primers. Forward Primer - name:RZPD M13 forward, sequence:GCTATTACGCCAGCTGGCGAAAGGGGGATGTG; Reverse Primer - name:RZPD M13 reverse, sequence:CCCCAGGCTTTACACTTTATGCTTCCGGCTCG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3326079 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14528 same embryo
	EMAGE:14529 same embryo
	EMAGE:14531 same embryo
	EMAGE:14530 same embryo
	EurExpress:euxassay_005383 same experiment
	MGI:4828762 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source