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EMAGE:14575

1700049L16Rik RIKEN cDNA 1700049L16 gene ( MGI:1920633)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14575 EMAGE:14575 EMAGE:14575 EMAGE:14575 EMAGE:14575
"Pseudo-wholemount" of euxassay_005425. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005425_01 euxassay_005425_02 euxassay_005425_03 euxassay_005425_04
EMAGE:14575 EMAGE:14575 EMAGE:14575 EMAGE:14575 EMAGE:14575
euxassay_005425_05 euxassay_005425_06 euxassay_005425_07 euxassay_005425_08 euxassay_005425_09
EMAGE:14575 EMAGE:14575 EMAGE:14575 EMAGE:14575 EMAGE:14575
euxassay_005425_10 euxassay_005425_11 euxassay_005425_12 euxassay_005425_13 euxassay_005425_14
EMAGE:14575 EMAGE:14575 EMAGE:14575 EMAGE:14575 EMAGE:14575
euxassay_005425_15 euxassay_005425_16 euxassay_005425_17 euxassay_005425_18 euxassay_005425_19
EMAGE:14575 EMAGE:14575 EMAGE:14575
euxassay_005425_20 euxassay_005425_21 euxassay_005425_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14575Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14575_wholemount_strong.wlz
14575_wholemount_moderate.wlz
14575_wholemount_weak.wlz
14575_wholemount_possible.wlz
14575_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14575_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9834
Entity Detected:1700049L16Rik, RIKEN cDNA 1700049L16 gene ( MGI:1920633)
Sequence:sense strand is shown

>T9834
ACAGAAGCAAGCTCACTCACGGTGGCAGCTGTTGAAGTGTGGGGGCCGCGGTCGACATGTTCCAGGGCAT
GGACAGCCACTCTGGCGGGTTGGGCTCCAGATCCATGAAGCCACCAGGAGGAGAATCGAGTGATCTTTTT
GGAAGTCTAGAAGAACGCCTTCCTTCAACACGCAAACCAAATAGGATGGCATCCAATATTTTTGGACCAC
CTGAAGAGCCTAAAAATATACCCAAGAGGACAAATCCACCAGGGGGCAAAGGAAGTGGGATTTTTGATGA
ATCAACTCCTGTAAAAACTCGCCAACGTCTGAATCCACCCGGTGGGAAGACCAGTGACATATTTGGGTCC
CCAATTACTGCCACTGCACCTCTGGCACACCCAAACAAGCCCAAGGATCATGTTTTGCTATGTGAAGGCG
AAGACTCTAAATCCGACCTGAACGCAGCAACAAACGCCACACCCAGGGGAGAGCATGCAAAGAAACCGGA
GCTAACACCTACAGTTGACAATCATGAGCCCAGACTGGGGCCAAGACCTCGCTCCCACAACAAAGTTCTG
AACCCACCAGGAGGCAAATCCAGCATCTCCTTCTATTGAGAGGTTCCTGCTCTGCCTGTAACTAGATTGG
ACACTCAGAGACAGGGTATTGAATGTTAGTGTTTCCTTTAACCCGAATGAGTTAGGGGTGGGAGAGGGTT
TGTCATGCACGTGGGATTGGCTGCTCATAGGCCTTGCTGTCATTGGAAGAGCTGCCCAGAGGACTGTGCC
TTCTAGACTGCCAAGATATAAATCTTCGGTGAAAAATAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6772617 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6772617 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14572 same embryo
 EMAGE:14576 same embryo
 EMAGE:14573 same embryo
 EMAGE:14574 same embryo
 EMAGE:14577 same embryo
 EurExpress:euxassay_005425 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS