EMAGE mouse gene expression spatial database

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EMAGE:14578

Crybg3 beta-gamma crystallin domain containing 3 ( MGI:2676311)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005427. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005427_01	euxassay_005427_02	euxassay_005427_03	euxassay_005427_04

euxassay_005427_05	euxassay_005427_06	euxassay_005427_07	euxassay_005427_08	euxassay_005427_09

euxassay_005427_10	euxassay_005427_11	euxassay_005427_12	euxassay_005427_13	euxassay_005427_14

euxassay_005427_15	euxassay_005427_16	euxassay_005427_17	euxassay_005427_18	euxassay_005427_19

euxassay_005427_20	euxassay_005427_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14578_wholemount_strong.wlz

14578_wholemount_moderate.wlz

14578_wholemount_weak.wlz

14578_wholemount_possible.wlz

14578_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14578_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8795
Entity Detected:	Crybg3, beta-gamma crystallin domain containing 3 ( MGI:2676311)
Sequence:	sense strand is shown >T8795 TAGACATGGTACCTGCCGAACTAGAAATGGAAAGTATTTATCCTAAGGACTCTGAAGGAGATACTGACAG TATCTCTGAGGGGATGCCTGCCAAGCTAGATGTGGTAAATGCTTATCAAAAATACAGTGACAGAGTTACT GGAAGGATCGAGAGACCTGTGTCTCCCCTAAGGGATGCTGAAGATGTCCTAGGAAATGCTGAAACAGTCC CATTTGTGCTAGATGTGAAAGAAACCTACCAGGAGACAGTATATCCTCCCTTGGACATCTTGGAAAAACG AATCCAAAGAGACACTGAAGAGTCAAATGGAACAACAGAGCCTGTGCCTTCTAAGATAGAAATGGAAGTA ATGGCTCCAGAGGATTCCAGTGGCAGCACTGAGAACCGTGAACTGTTACCTATGGAGGTAGCTATGGAGC GCACCAATGGAACAACACCAGGGGTGACCATTATGCAAACGGACGCCCTAGGTCCTGCCTTTGAAAATAC AAAGGATCCTCGGGAGTACATGGAGAAGAGCATTGGAGAAATAGAAGAACCGCCTGGTGAGGTAAAAAAA GGCTTGATCTTGCACGATGACAGACTTGCTTCGCATTTCAGGGGCTATGAGTCGCCTACATTAAGTAAGG ATTATGAGGGCTACCCAGCCTCAGCAATACCAGATGTCCAAGAGGAGGACACAGTAGTAAGGCTCAAGAA AATAATGTCTGTCCCTGTAGTATATGACAAGAGGAGAAATCTGGATTGTAGAGAAGAAAAGGAAGAGTCC AACTTAGCCTTTGTCTCTCAAGACGAACAAGACAGTTCTTCCTTTACCATATTGTATGAGGAACCCCTTC AAGAGGAGGACAGGTACACCTCTGCAGAGTTACGAGGGTCACAGTCTCTCCTGTTTCCCGACACCTCCAG CATGCCTGGCCTGGCCTGCGAAAGGTCTGAGAGCAGAACTGACCTGGTCCATCATTTTGAAAAAGAAGGT AAACTGGGTGAGGCATTTGATGGAGACAATTCAGAGATGTTCTTATCGGTGGAAGCCAAGAGGTACAAAA TCTATCCCTTAGCCCTGAGCCCCATCTATGAGGATGACAGTTCGCAGGAGGATGTTCTGTCCAGTGAGGT CTCGCCTGGTCACCATGGCTCTTCCAAGTCAAGGGAGAGTGCAAACCAGCCCTCTTCTGTCTTATCCCTC CTACAGTCAGTGTCAGAGCGCTTACAGAGAAACTTCGATGGAGATGACAGACAGGAGGCCGAGGAAGAGG AGGAGGAAGCTGTAGCTTCGGGCAAAGATTGGAGAACTGAAAAGAGGGAGCATGTTACCTTCCACTTGCC AGATCCTTCCATCCCCTTTTACCCTGAAGACAACCAGGAACACGCTGGAATTTTCAAGTCTTATGTAGAG TTCAGTGAACCAACGACCTCCAGTCTGCAGCATGGCCGGTGGTCAGAAAAGGAGTTGTTTCTCCAAAAAT CCGATATGACTTCTAAGTTGCATTCTTCTCTGAAGAGTGCCTATCATCAGTACTTGCAGACTTCCAGGAC TCACTCCTCAGAGACGGGGACCAGATTTGGTGGCACCCTTCAGGAACCAGTGTCCAAGTATTTCCGTGTT CAGGACCATGCAGGCAGACTGAGTCCGTATGTGGAGAACGTTGACAAACAAACTCTGAAGTGTAATCCAA GGCCTGGAAAGATGATAATTTATGATCTCCATGGAAGTAAATATAAACAAGAGGTCTACTGTAACATCCC TGATGCTACAACATGGTCTTTCCCGAATGGGGCACTGATAAAAGTTGTAAGGGGCTGCTGGATTCTGTAT GAGAAGCCCCATTTCCAAGGTCAGAAGTGTGTGTTGGAAGAAGGGGAAAGAGTGTTAGACCGGGACTGGC TTCTTCAGAACAGGAAGCACCCAGAAAGGAACTTTGTCCTGGGCTCTATCAAACGTGTCTTAAAGGATTG CAGCATTCCGGTGATAGAGCTGTGCCCCAAGACTGACCCTGGGTGCAGCCCCATCTACATACACCGATCT GTCCCCAATGTGGAAGAGCTGAACATCCCCAAGTCTACATCCGTCACTGTGAAGTCAGGCGTTTGGCTGG CCTACCCAGATATTCATTTTAAGGGGCAAGCTACAATTTTGGAGGAAGACCAGGGGCTCTTTGAGATTTC TGCAGCCGAGATGAAATCACTGCATCCTCTTCAAATGGGTGGACTGAAAGTGGAAATGCCTATGAACTTA AAGGTTATCCTTTATGAGAAACCTCACTTCCTCGGACATACCAAAGAGTTTAGTGAACATATAGATTCTG TCCCTACGTTTTTAAAAAGCGATAAGGACTTCCATGGCATCGGATCGATTCGTGTCATTGGTGGAGTGTG GGTTGCCTATGAAAAGGAACATTTCAAAGGCCAGCAATTCCTACTAGAAGAAGGAGACTTTGAAGATAGC AGCGCTTGTGGGGCCTTAAGTGGACCCATCATGTCTTTCCGGTACCTACAAGCTAATTTTATAGAATCTT CGATCACACTGTTTGAATCATCTCACCTGGAAAGTGGGAAGTTTATTGACATCACGAATCAGGAAATTTC TGACTTAGAAGAAATCGGCTTTGGCAGCGAGACAAGATCCATTCACGTGAAGAGTGGAGTGTGGGTTGCC TACCACCAGAAGTTCTTCTGCGGCGATCAGTACATTTTAGAGAAAGGAAAATATAAATGCTTTTTTGATT GGGGAGGATCAAGTAATACAATCTTGTCTATCCGACCAATCCAATTGGAACCACTCGGGATAAATGAGCC TACACACTTGCTCAAAGCATTCAGTAAAGCAGGCTTCCAAGGAGAGTGTATCGATTTCGTAAAAGAATGT GCCGACCTGACCTCGTTCACGCCGGCATCCTTTAAGGTTCTCCGTGGCTGCTGGCTCCTCCTGTATTACC AAGAAGACGGTTTCTATCATCAGTGTGTACTGGAAGAGGGCCTCTACGTTGACCTCACTTCCTGCGGCTG CCCATCAGCTAGAATCAGAGCACTCCAGCCCATCGACTATGTTTTTGAAGAACCTTCCATCAGCCTTTTT GCTCTGGAACATTGTGAGGGAAGAGAACTACATCTTGAAGACGCCGTGAATTCCGTTTTGAACAAAGACC TACACTTCTACACCCAGTCTGTGTGGATAAAGAGTGGCTTATGGATCGCTTATGAAGGTTCCAATTTCTT GGGAAGGCAAATCCTTCTTACGCCTAAGGAGATTCCAAATTGGACAGCATTCAGTGGGTGGAAGACAATC GGCTCTGTGCGTCCTATGAAGCAGCCTGCAGTGTACATCAGAATAAGGAACCGAGCCCAGGATGAATACT TGACAGTCACTGGGAACCCAGCAGATGCACGGACAATGTCTGTCTGCATCTCTCCCTACAGTGGGAAGGA CACTCAGATCTGGCACTACTGCCGAGGACTCTTTAAATCCAAGGCCAGTCATACATGTCTTGATGTGATT GGAGGCCGGGACACTCCTGGAGCCAAGGTGGCCTTGTGGACTGAACACGGGCAGCTCAGGCAGAAGTGGA GAATGAGCAGGAACGGAACCATCAGCTCTTATCTCAGTGATGAACTCGTCCTGGATGTTAAAGGAGGAAA CTATTATGACAAGACTCATGTCATTGTGAATCAGCCCCTGGAAGGGGAAGAGACCCAGAAGTGGGACATT GAAATCCTGTGAGAGAATCACAAAGGCACCTCCACCCACTCACGTGCTGTGTGCATGAGAAGGAGGCCGC TGTGTTCACACTACTGCACCGCACCGCACCCAGAAAGAACTTTCTCCCCAGCGGTGACGACTGCTCCATG GGGAAGCTAAAATATCCTCGCCAGTTCCGGAGCACCCTTGTGAAGAAAATGTGTTCTTTCTTCCGATGGG TCCAGTTGCAGTGGCCAATTTCCTGAAACCTGTCTTTTCCTAAGCGCTGACCGTGAACTCTGCCCTTGAT GGCGCTAAGCTGTCTGGACAGTGCGGTTACAAGCATTAGTGAGTGTCCTGCTCCACGCCTGTGTGAACCA GGCACAGGAAAACAAACTCCTACAGGCTGTCATGCTCCTGCAGGAAGATGAGTCATTTTTAGCACCACCT GAAGACTTTTATTTAAAACAGCTTTCATATTAAACTGCTGCTGTATTAAACAGACCTTAGTTTCCATATA GAAACTTTATAACTGAAACACCCTTAAGTATTTATATTCTTTAATTATCTGACCCACAAGGAGGGACCTC GGCTTCTTGAGGCAAAAGGCACTAGTACTTAAAGTACTTTCTGTGGCGTGACCAATCTTTACTGTTTTTC TAACTACAGATGTATGAACATCCTTGGAGTGCCCACCCAGCACCCAGTGTTGAGTATTAACCTTACATTT TACCCGACTTAAGGAGGAATAGTAGTGTAAGAGCTGCCCGGCTCCCTGGAGTAAAGCGCTACACGCAAGT TCCCATAAAGCAAACAGGGCTTCTACTTTGTCTGCTCGGTGACAGACAACCAAGTTTTCCTCCCATTTTA AACAATTACAGCTAAGCAAAACCAGGTTTAGAGGCACTACCAGTACACACAGTACCTGCACACGTACAGG TCAACAGCCTTCTCCCTCCTAAGATGGACTTAATTCAGACAGCCACTGCTTTCAGCCCCGATTTTGGCCA GAAAGAAAAAATACTTATTTTTACAGAAATTCTCCAGCAATATTTTTAAAAATACCAACTTGTATGCACT TTAGAATGTAAAATAACAGTGGATACTTAAGACTCAAAGAGCCACCACTACTTTATTTTTTATACACTTG GTCTTTCCATGTATATACCGGCCTCTGTTTTTTTTTTTTCCAATCTCTGGCTCTTCTGTCATCTTCCAAG CAGCGTCTGAGAGCTCTGCACGGGCACAGATGCTGGGTACCTCTTATGGGTGTGGCAGTATTACACGAAA CATGTTTATTTTAATGGGGAAAGGGGTCATGTATGCTCAAAGATATGTATATTTTCATTGTAAAGAAAAA AATCCAACTTTATTCCTAAGTTTTAATTGTTAGAAAGTATTTTCGGAGCCAGTGATGCTCTTTAAAGGAG TTATTTCTTCCAAAGAAGCAAGACGAGCCAGGTTACTGACATAATCTTTATGACATGTGTACTGCAGTTG TGCCTTTTCTACAACACTGCATTGAATAAACTTGTCCGAAGTAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6408859 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6408859 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14583 same embryo
	EMAGE:14581 same embryo
	EMAGE:14580 same embryo
	EMAGE:14579 same embryo
	EMAGE:14582 same embryo
	EurExpress:euxassay_005427 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source