EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14590

Chrac1 chromatin accessibility complex 1 ( MGI:2135796)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005461. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005461_01	euxassay_005461_02	euxassay_005461_03	euxassay_005461_04

euxassay_005461_05	euxassay_005461_06	euxassay_005461_07	euxassay_005461_08	euxassay_005461_09

euxassay_005461_10	euxassay_005461_11	euxassay_005461_12	euxassay_005461_13	euxassay_005461_14

euxassay_005461_15	euxassay_005461_16	euxassay_005461_17	euxassay_005461_18	euxassay_005461_19

euxassay_005461_20	euxassay_005461_21	euxassay_005461_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14590_wholemount_strong.wlz

14590_wholemount_moderate.wlz

14590_wholemount_weak.wlz

14590_wholemount_possible.wlz

14590_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14590_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9858
Entity Detected:	Chrac1, chromatin accessibility complex 1 ( MGI:2135796)
Sequence:	sense strand is shown >T9858 GGCCGCCCGGGACGGACGCGGAAGATGGCGGACGCGGCCGTGGGGAAGGAAAAGTGCGGGGACCAGCGGC TCGTGTCATTGCCCCTGTCCCGCATCCGAGTCATCATGAAGAGCTCTCCCGAGGTGTCGAGCATCAACCA GGAGGCGCTGGTGCTCACGGCCAAGGCCACGGAGCTCTTTGTTCAGTACCTAGCCACCTGTTCCTACAGA CACGGAAGTGGCAAAGCCAAGAAAGCACTGACGTACAGCGACTTAGCCAGCACTGCAGAGGACTCGGAGA CACTTCAGTTTCTCGCAGATATATTACCAAAGAAGATTTTAGCTAGTAAGTACCTGAAAATGCTCAAAGA GAAGAGGGAAGAGGAGGAGGACAATGAGGACGATGGAAGCGACCTTGGTGAAGCCCTGGCTTAGGCCAGC AAGAAGAGCTCTCATCCTGTCCTGTGAGCACATGCAAGGACAGACTAACAGCTGCGAAGTGAAGGGCACT GCAACTTTGTCTCAGACTTGCAACTTGTTGCATTCCCTCTTGCAGTGGAAAGGACTGGACAGCAGCAGCC TCCTCTACAGGCCCTTCAGCCATCTCCTCTCTCCCACTGGGAGAGTCCTGGGCAGATGGCACTGCTCTGG CTTTGGGTACAGTAGCACACACCACTGAACCTCACATTGAACTTGCAGGGCAGACCTGGGAAGCTCCCTG GCAGCTGTGTAGTCGCGTGGACACACTGTTCTCACAGACTACATGCATACTGTGCTACCTTTGATGTTCG CTTTCTAAAAAAAGGACTATGCAGTTTTATTGTACAGGAATTTGCTTTCCTCTTTCCATTAAAGGTCTGT ATTGTACAGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6773451 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6773451 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14592 same embryo
	EMAGE:14593 same embryo
	EMAGE:14589 same embryo
	EMAGE:14591 same embryo
	EurExpress:euxassay_005461 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source