EMAGE mouse gene expression spatial database

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EMAGE:14592

1700027A23Rik RIKEN cDNA 1700027A23 gene ( MGI:1923670)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005451. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005451_01	euxassay_005451_02	euxassay_005451_03	euxassay_005451_04

euxassay_005451_05	euxassay_005451_06	euxassay_005451_07	euxassay_005451_08	euxassay_005451_09

euxassay_005451_10	euxassay_005451_11	euxassay_005451_12	euxassay_005451_13	euxassay_005451_14

euxassay_005451_15	euxassay_005451_16	euxassay_005451_17	euxassay_005451_18	euxassay_005451_19

euxassay_005451_20	euxassay_005451_21	euxassay_005451_22	euxassay_005451_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14592_wholemount_strong.wlz

14592_wholemount_moderate.wlz

14592_wholemount_weak.wlz

14592_wholemount_possible.wlz

14592_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14592_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

lateral ventricle choroid plexus

weak

homogeneous

weak expression: see section 07 08 09 10 11 13 14 16 17 18 19

4th ventricle

weak

homogeneous

weak expression: see section 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9857
Entity Detected:	1700027A23Rik, RIKEN cDNA 1700027A23 gene ( MGI:1923670)
Sequence:	sense strand is shown >T9857 ATTCATTTTCTTGTTTAAAGCCTATGAAGGAAGTAGTGGCTCATGAAGACAGAAAATGAGGAGGACGTCA AGCCCCCTGAAAGTTGTCATGCAATGCAGTTACTATTCAAATCTCTTGAGGCATCTGAAAGGGCTAAGGT AGAAGAAGAACTGAAAAAGACCAAAGAGAATGAATTCACACAACGGAATGAACATCATGCTCTTCGGGAC ATCACTCATGGGAAGGTGAATAATTACTCCTTTGGAACACGTCAAGCGAGGAAACTCTTTCCTCACTACC ATCCCCCAACCTGGCTGGGGAACCTGTATCTCCCTCTTAGGGGAATGCCCCACACAGGCCCTGGGTGTTA TGCAGCAGCAACAGATTGGAATGGTTTGGCATATAATCTATCGAAAGTCCCAACCAGTACGAAAGGCTAT GCTATTGGAGCCAGGACAGCTGTGAGGTTTAAGCCAATCAGCAAGGATGTGACACCATACCCAGGGATGT ATCAGAAAGTTGATACTTTGAGTGAAAAACACAAAAAAAGCTTTGCTCCATTTAATATCTTGATGCCTCG ATTTAGGAGTGCAGCAAAGGGTGATTCTTACCCCGGCCCTGGCACATACAATCCAGAGATGAAGTCAGTC CCAAAAGTTACTTGGCCAATGAAATTTGGATCTCCAGACTGGTCTCAGGTTCCATGTCTAGAGAAAAGGA CTCTAAAGGCTGAGCTGTCCGCAGACAAGGACTTTAGAAAGCATCGTAGCCGTGTGGCCTACTTTAGCCT ATATTACCAGTGAAATGTAGGCACTGTCTTCACGTGCTGTCCTGACCACAGAGTCTCCAGAACTGAGGAC ATAATTGCTCCTGTATTTCTGTGTTCCTCGGCCCTCTTGTCTGCCCATTTCGTGTCCAGAGAGGGACAGG GTCTCATTGTCACCCTCCAAATACATAAAGGCTGGTTCAGAATGTTCTAAAAAAAAATAAAAAAAAAAAA AAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6773491 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6773491 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14590 same embryo
	EMAGE:14593 same embryo
	EMAGE:14589 same embryo
	EMAGE:14591 same embryo
	EurExpress:euxassay_005451 same experiment
	MGI:4822627 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source