EMAGE mouse gene expression spatial database

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EMAGE:14597

Ninl ninein-like ( MGI:1925427)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005501. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005501_01	euxassay_005501_02	euxassay_005501_03	euxassay_005501_04

euxassay_005501_05	euxassay_005501_06	euxassay_005501_07	euxassay_005501_08	euxassay_005501_09

euxassay_005501_10	euxassay_005501_11	euxassay_005501_12	euxassay_005501_13	euxassay_005501_14

euxassay_005501_15	euxassay_005501_16	euxassay_005501_17	euxassay_005501_18	euxassay_005501_19

euxassay_005501_20	euxassay_005501_21	euxassay_005501_22	euxassay_005501_23	euxassay_005501_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14597_wholemount_strong.wlz

14597_wholemount_moderate.wlz

14597_wholemount_weak.wlz

14597_wholemount_possible.wlz

14597_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14597_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

liver left lobe

moderate

regional

moderate expression: see section 01 02 03 04 05 06 weak expression: see section 07 08 09 10 11 12

liver right lobe

moderate

regional

moderate expression: see section 20 21 22 23 24 weak expression: see section 13 14 15 16 17 18 19 not examined expression: see section 10 11 12

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9595
Entity Detected:	Ninl, ninein-like ( MGI:1925427)
Sequence:	sense strand is shown >T9595 GGGACGCGCGCTGGGGTGTCCTACCCGGCTCGCTTTGCACCGGGCTGCCGGAGCCCGCATCCTGAACTCA GCGTTCAGAGAGCCGAGCCTCCCACTCCCCAGCCTGCTATGGGATGGACAACGAAGAAGAGAACCACTAT GTGTCACGGCTCAGGGATGTCTACAGCAGCTGTGACACCACAGGGACCGGGTTCCTGGATCAGGAAGAGC TGACCCAGCTATGTACTAAGCTTGGCCTGGAGGAGCAGCTGCCGGCACTCCTTCACATTCTTCTCGGAGA TGACCGCTTGGCCAGGGTTAACTTTGAGGAATTTAAGGAAGGCTTTGTGGCTGTGCTGTCATCAGGTTCT GGCGTAGAGCCCTCCGATGAGGAAGGTAGTTCTTCCGAGTCAGCTACTTCCTGTGCTGTCCCTCCAAAGT ACATGAGTGGCTCTAAGTGGTATGGCCGTCGGAGCCTACCTGAGCTCGGTGACTCTGCCACTGCAACCAA ATATGGATCAGAACAGCAGGCCAAGGGCAGCGTGAAGCCTCCGCTGAGACGCTCTGCATCTCTGGAAAGT GTGGAGAGCCTCAAGTCAGATGAAGATGCTGAGAGTGCAAAAGAACCTCAGAATGAATTATTTGAAGCAC AAGGCCAGCTGCGATCCTGGGGTTGTGAGGTCTTTGGGACACTCCGGAAATCCTGTAGCCCCTCCTTCAG CACTCCTGAGAACCTGGTCCAGGGCATCTGGCATGAGCTGGGGATTGGAAGCAGTGGTCACCTGAATGAG CAGGAGCTGGCTGTGGTCTGCCGAAGCATCGGGCTCCACAGTCTTGAGAAACAGGAACTTGAAGAACTGT TCAGCAAACTGGACCAAGATGGAGATGGCAGAGTGAGTCTTGCAGAGTTTCAGCTTGGCCTGTTTGGTCA TGAGCCTCCTTCGCTTCCAGCATCTTCCAGTCTGATCAAACCAAACAGGCTTTGGTCTCATTATCAGGAG GAGAGCGGCTGCCACACCACCACAACCTCGTCCCTCGTGTCTGTGTGCTCAGGCCTGCGCCTCTTCTCCA GTGTCGACGATGGCAGTGGCTTTGCCTTTCCTGAGCAGGTCATCTCCGCATGGGCCCAGGAGGGCATTCA GAATGGCAGGGAGATCTTGCAGAGCCTGGACTTCAGTGTGGATGAGAAGGTGAACCTCTTAGAGCTGACC TGGGCCCTTGACAATGAACTGCTAACTGTGGATGGTGTCATCCAGCAGGCAGCCCTGGCCTGCTACCGCC AGGAACTGAGCTACCACCAAGGGCAAGTGGACCAGCTGGTGCAGGAGCGGGACAAGGCGAGGCAGGACCT GGAGAAAGCTGAGAAGAGGAACCTGGACTTTGTGAGAGAGATGGATGACTGCCACTCAGCCCTGGAGCAG CTCACGGAAAAGAAAATCAAGCACCTAGAGCAAGAGTACAGGGGAAGGCTGAGCCTCCTGCGGTCCGAGG TAGAGATGGAGCGAGAGTTATTCTGGGAGCAAGCCCGCAGGCAGAGAGCCGTGCTGGAGCAGGATGTGGG CCGTCTCCAGGCTGAGGAGACCAGTCTCCGAGAGAAGCTGACATTGGCTCTGAAGGAAAACAGTCGATTG CAGAAGGAGATCATAGAAGTGGTAGAAAAGCTTTCGGATTCAGAGAAGCTGGTCCTACGCCTGCAGAGTG ACCTCCAGTTTGTGCTGAAGGACAAGCTGGAGCCTCAGAGCATGGAGCTCCTGGCCCAGGAGGAGCAGTT CACAGCCATCCTGAACGATTATGAGCTTAAGTGCAGGGACCTACAGGACCGCAATGATGAGTTGCAAGCA GAGCTGGAAGGCCTGCGGCTGCGGCTGCCCAGGAGTCGACAAAGCCCCGCTGGGACCCCAGGTACCCATA GAAGGAGGATCCCTGGACGCGGCCCAGCAGACAATCTGTTTGTGGGTGAGTCTACGCCAGTGAGTTTGGA GACAGAGATAATGGTGGAACAGATGAAAGAACATTACCAAGAGCTCAGGATGCAGCTGGAGACCAAGGTA AATTACTATGAGAAGGAAATTGAAGTCATGAAGAGAAACTTTGAGAAGGATAAAAAGGAGATGGAGCAGG CTTTCCAGCTGGAGGTCAGCGTTCTGGAAGGCCAGAAGGCAGATCTGGAGGCCTTGTATGCCAAGTCGCA GGAGGTCATCCTGGGCCTGAAGGAGCAGCTGCAGGATGCAGCACAAAGCCCTGAGCCTGCCCCAGCTGGA CTGGCCCACTGCTGTGCACAGGCCCTGTGCACACTGGCCCAGCGGCTAGAAGTGGAGATGCACCTGCGAC ACCAGGACCAGCTACTGCAGATAAGGCAAGAGGCTGAAGAAGAGCTGAACCAGAAGCTGTCATGGCTAGA AGCTCAGCATGCTGCCTGCTGTGAGAGTCTGTCTCTGCAGCATCAGTGTGAGAAGGACCAGCTGCTGCAG ACACACCTGCAGCGAGTGAAGGACCTGGCTGCTCAGCTTGACCTGGAGAAGGGGCGGCGGGAGGAGAGGG AACAGGAGGTCCTGGCACACTGTCGGAGGCAGCAGTTGAAGCTGCAGGCTGTGATGAGTGAAGAGCAGGC ACGGATCTGCAGGTCATTCACCCTGGAGAAGGAGAAGCTAGAACAAACCTACAGGGAGCAGGTAGAGGGC CTTGTCCAGGAGGCAGATGTGCTGCGTGCTCTCCTGAAGAATGGAACCACTGTGGTGAGTGACCAGCAGG AGAGGACACCCAGCTCCATGTCCCTGGGTCCAGACAGCAGGCAGCAGCCAACAGCCCGGCAAGCTGTCAG CCCTGATGGAAGGACAGGGGCACCAGCAGAGTGGCCTGGCCCAGAGAAAGCAGAGGGCAGGGATTTTCCT GGCCAGCTCTGTAGTATAGATGCCATGCCAAGCCCAACCCCTACCCTGCTGTCTAGAAGATCCTCAGAGA ACCTGGGTGTCAGAGACAATCATCAACGTCCACTCAATGCTGAGGAGGGAGCTATCCCAAAGGAGCCAGA ACCTTCTGCCAGGACCCTAACAGGCCAAGGCCAGAAGCTGCCCTTGCCTGTTCATCCACAGATGCTGGAG CAGCAGGGTGATCAAATCCAAAACCTGAAAATTGAACTTGAACGTGTGAATGAGGAATGCCAGTACTTAA GACTGTCACAGGCAGAGCTGACAGAAAGCCTTGAAGAAAGTCGAAGCCAGCTCTACAGTGTCCAGCTGAG GCTGGAGGCAGCACAGTCCCAGCATGGCCGGATTGTCCAGCGCCTGCAGGAACAGATGAGTCAGTTGGTT CCCGGAGCTCGTGTAGCCGAACTGCAGCACCTGCTCAACGTCAAGGAAGAGGAAGCCAGGAGACTGAGTG CACAGCAGGAAGAATACAGGCAGCAACTGAAGGCCAGGGAGGACCAGGTGGAGGATGCCGAGGCTCGGTT ACGCAACGTGGAGTGGCTGCTACAGGAGAAGGTGGAGGAGCTTCGAAAACAGTTTGAGAAGAATACCAGA TCTGATCTGCTGCTTAAGGAACTGTATGTGGAAAATGCCCACCTCATGAAAGCTGTTCAGCTCACAGAAG AGAAGCAGCGAGGAGCCGAGAAGAAAAACTGTGTCTTGGAAGAAAAAGTTAGAGCTCTCAACAAGCTGAT CAGTAAGATGGCACCAGCATCACTTTCCGTGTAGAGGCTGCTTTTCTTGGAGTTCATGTGAAGGAATGTC TTTTTAAATAATGCTACTCTGATGCCATAATTTTCTATGGCTCACTAGCTACACTTCCTAGACATGAGGA CTGATTCCTGTTCACACAACAAATATTCAGTGAATGTCTACTATGTGCCAGGTTCTGGGGAAGCAGGCAG GGTACAGTCACTTTTGGCCAAGGGTCTTGGGTAACATCCTGGGTTGAAGTTGCCAAGCTTCATCTTACTG AAAAAGACTCAGACCCTCAGCAAAGAACAATGGAGAGAAAGTAGGTGAGTACTTTGAGTGTTGGGCTTTG GGCCAAAAATGCAGACTCAGGTTTTAGGTGTCTTACCACATGGCTGCCCTGTGCAGGCTGTCATCAGAGT TCTCCATGTGGGTCAATACCTGCACCCCCAGGAAGAGGGCATTCCCCAGCAGGCCTCTGCAGATCCTGCG GGTGGGAGGAAAGACGGCATCAGAGGTGGTGGCTCAGGGATAGCAGTGTTCGTTGCGAGTGCAATCTCAT TACAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30615484 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30615484 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14594 same embryo
	EMAGE:14596 same embryo
	EMAGE:14595 same embryo
	EurExpress:euxassay_005501 same experiment
	MGI:4826713 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source