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EMAGE:14612

Cox17 cytochrome c oxidase, subunit XVII assembly protein homolog (yeast) ( MGI:1333806)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14612 EMAGE:14612 EMAGE:14612 EMAGE:14612 EMAGE:14612
euxassay_005462_01 euxassay_005462_02 euxassay_005462_03 euxassay_005462_04 euxassay_005462_05
EMAGE:14612 EMAGE:14612 EMAGE:14612 EMAGE:14612 EMAGE:14612
euxassay_005462_06 euxassay_005462_07 euxassay_005462_08 euxassay_005462_09 euxassay_005462_10
EMAGE:14612 EMAGE:14612 EMAGE:14612 EMAGE:14612 EMAGE:14612
euxassay_005462_11 euxassay_005462_12 euxassay_005462_13 euxassay_005462_14 euxassay_005462_15
EMAGE:14612 EMAGE:14612 EMAGE:14612 EMAGE:14612 EMAGE:14612
euxassay_005462_16 euxassay_005462_17 euxassay_005462_18 euxassay_005462_19 euxassay_005462_20
EMAGE:14612
euxassay_005462_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14612Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14612_wholemount_strong.wlz
14612_wholemount_moderate.wlz
14612_wholemount_weak.wlz
14612_wholemount_possible.wlz
14612_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14612_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9877
Entity Detected:Cox17, cytochrome c oxidase, subunit XVII assembly protein homolog (yeast) ( MGI:1333806)
Sequence:sense strand is shown

>T9877
GTCCCCCTTCGATTGACTGCGGAAGTGGAGACAGACGGCTCAGGGTAGTCGGAGTTTGGGAGCTTTGCGC
GTGTGCGCTCATAGTTGCTTTCGCCTGGAAAGATGCCGGGACTGGCGGCCGCTAGCCCTGCCCCGCCCGA
GGCTCAGGAGAAGAAGCCACTGAAGCCCTGCTGTGCCTGCCCGGAAACCAAGAAGGCGCGTGATGCGTGC
ATCATTGAGAAAGGAGAAGAACACTGTGGACATCTCATTGAAGCCCACAAGGAGTGCATGAGGGCACTGG
GATTTAAGATATGACGTGGTGGCCTACTTTGTGAATGAATAATCCTTGAAGAATGAAGAAGATTAAATAT
TTTGGAAGTTCTTTGGAGAACTTTGATAAATGGATAAAGTATTTATAATTTATTAAAAAACAGAAGGAAA
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAA
Notes:The probe template was PCR amplified from IMAGE:6773943 using vector (pDNR-LIB) specific primers. Forward Primer - name:M13 forward (-21), sequence:TGTAAAACGACGGCCAGT; Reverse Primer - name:T3-pDNR-LIB rv, sequence:CAAATTAACCCTCACTAAAGGGTGTTCACTTACCTACTGGGC. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6773943 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14615 same embryo
 EMAGE:14611 same embryo
 EMAGE:14614 same embryo
 EMAGE:14613 same embryo
 EMAGE:14610 same embryo
 EurExpress:euxassay_005462 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS