EMAGE mouse gene expression spatial database

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EMAGE:14634

Zfp948 zinc finger protein 948 ( MGI:3040683)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005488. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005488_01	euxassay_005488_02	euxassay_005488_03	euxassay_005488_04

euxassay_005488_05	euxassay_005488_06	euxassay_005488_07	euxassay_005488_08	euxassay_005488_09

euxassay_005488_10	euxassay_005488_11	euxassay_005488_12	euxassay_005488_13	euxassay_005488_14

euxassay_005488_15	euxassay_005488_16	euxassay_005488_17	euxassay_005488_18	euxassay_005488_19

euxassay_005488_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14634_wholemount_strong.wlz

14634_wholemount_moderate.wlz

14634_wholemount_weak.wlz

14634_wholemount_possible.wlz

14634_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14634_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9608
Entity Detected:	Zfp948, zinc finger protein 948 ( MGI:3040683)
Sequence:	sense strand is shown >T9608 AGGACCTGTCAGGTCCAGAGCTCGCGGCCCTGCCCCGTGGGGAATCGCGTGGTTCAGACCTGGGGTCAGA GGACACAGAGGACTTCGTGGGAAGCTAAAACTCCAGGCAGCCGCGGGTGCCTTGCGGGGCATCCTGGAGG AATCAGCAATCAGTCGCAAAGCGGCGAGAACCGGAGCCAGGCGGCCCTGGAACCGCCCAGGGTTCGCTGA TCACCCAGACCTGCTTATCTGTGGAAACCTAGAAGGTGGAGAGGGAGAACACAGTAGCTAAGGATCCAGG ACTACAAGCTGGCTATTCATCATTCCTGACAGAGAAATTTAAAGAAGAGAAACAAACAAACAAAAAAAAA TGGCTGATTCTCCAGTAAACATGTCCCAGTTTGACCCATTGATATTAAAGGACCACTAGCCAACTATTCA TTCTTCCTCAAAGAGAAAATCAAAGAAGAGACACAGAAAGAAATGGCTAAATCTCCAGTAAACATGTCCA AGCATCTGTTAACATTTCGAGATGTGGCCGTGGACTTCTCCCAGAAGGAGTGGGAGTGCCTGGACTCTGC TCAGAGGGCTTTGTACATTGACGTGATGGTGGAGAATTATAGCAACCTGGTGTTTGTGGAGAAATATTCT GTACGTGATACTGTCCATCAACATGTGAGGACTGAAAAGGAGTCTCGTCAGTGTAATGAACTTGGTGAAA TGCTTCATGAACCCACCAACTGTGCACCTTATAATACAAGTGACACTACAGAAACCTCTAACAACTACAG ATGCTGTAATGACCAGGATGCCTCTGTTGACTCTTCAAACCCAGATAGACTTAAAAGCGTGCACACTGGA GAAGAACGTTGTGAATATAAAGACTGTGAGAAATCTTCCAGCATTGCTCAAGATCAGAGAACAAACAGTG CAAACAAAGAACAGAGACAGGAAAAACATGGTGGCCATTTCAGCTCTACACACAGTCTCAAATCTTCCAG CATTGCTCAAGATCAGAGAACAAACAGTGCAAACAAAGAACAGGGACAGGAAAAACATGGTGGCCATTTC AGCTCTACACACAGTCTCATGCAACAAGCAATCTTCAATAGAGAGAAACGATACCAGTGTGGGCAATGCC AGAGATGCTTCAGTACCTCCGCAAACCTCACTATACATCAGAGAATTCATACTGGTGAGAAACCCTACAA GTGCAACTTTTGTGACAAATCCTTTGCCCAGTGTACAACTCTTAAAACACACCAACGTCTTCATACTGGA GAGAAACCTTACAAATGCAAAGAATGTGGAAAATCATTTCCTCAGTTGTCAGCACTTAAAAGCCATCAGA AAATGCATACTGGAGAAAAGCCTTACAAATGTAAGGAGTGTGACAGATTCTTTGCCCACTGTTCCAGTTT CAGGAGACATCAGAAAACTCATAGAGCTAAGAAACATACTGGCCCAGATTGTGGTAAAGACTTGCATCAG CTTTCACACTTTAAAAGCGATCACAGACTCCATACTGGAGAGAACCCGTACAAATGCAGTGAGTGTGACA GATCCTTTCCCCACTATGAATTATTTAGAATTCATCAAAAAACTCATTCTTCAGAAATAGATTATGAATG TGAAGAATGTGGTAAGTCCTTTACTGAATTATTACAACTTAGAAGGCATTACAAAATCCATTCTGGTGCA ACACTTTACAAATGTAGAGACTGTGACAAATCCTTTACCCAGTATTCACATCTTCAAAAACATCAGACAA TTCATACTGGAGAGAGACCTTACAAATGTAAGGATTGTGAAAAATCCTTTCTTCATAACTCACTTCTTAG ACGACATCAGAGTGTTCATACTGGAGAGAAACCTTATAAATGTAAGGAATGTGACAAATCTTTTACTAGA AACTCCATTCTTAGACAACATCAGAAAATTCATACTAAAGAAAAGCCTTACAAATGTGTAGAATGTGACA AGTCCTTTACCCAGCTCACATATCTAAGAACACACCAAAGAGTTCATACTAAAGAGAAACCTTACAATTG TACAGTATGTGACAAGTCCTTTACAACGGGAGCAACTCTTAGAATACATCAGAGAGTTCATACTGGAGAA AGACCATACAGATGTACGGAATGTGGCAAATCTTTTCGTGAAGGCACAACTCTTAGAACACATAAGAAAA TTCATACTGGTGAGAAACCTTACAATTGTACTGTATGTGACAAATCTTTTATCAGGTACTCACATCTCCA AAGACATCAGAGAGTTCATACGGGAGAGAAACCTTACAAATGTATGGAATGTGACAAATCTTTTACTGAG TGCTTTCTTCTTAGAAAGCATCAGAGAATTCATACTGAAGAGAAACCTTACAAATGCAAAGACTGTGACT TAGCCTTTGTGTGTATTTCAAATCTTAGAAAACATCAGAAAAATCATACTGGAGAAAAACATACTGTTGT AAATAATATGACATAACATTTATGTGGTGTTCCCTGCTTACAAATCACAAGGGAATAAGTAGTAGACTCA CAAAGATAACAAGTTTGTGAAATCCTGTAATGAAGATTGAAGTCTTTAAAAATGTAACAAAGTTTATATT AAAATAAAAATTCTGTAAATGTAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:30659397 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:30659397 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14631 same embryo
	EMAGE:14633 same embryo
	EMAGE:14632 same embryo
	EMAGE:14635 same embryo
	EurExpress:euxassay_005488 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source