EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14670

Mtap4 microtubule-associated protein 4 ( MGI:97178)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005584. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005584_01	euxassay_005584_02	euxassay_005584_03	euxassay_005584_04

euxassay_005584_05	euxassay_005584_06	euxassay_005584_07	euxassay_005584_08	euxassay_005584_09

euxassay_005584_10	euxassay_005584_11	euxassay_005584_12	euxassay_005584_13	euxassay_005584_14

euxassay_005584_15	euxassay_005584_16	euxassay_005584_17	euxassay_005584_18	euxassay_005584_19

euxassay_005584_20	euxassay_005584_21	euxassay_005584_22	euxassay_005584_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14670_wholemount_strong.wlz

14670_wholemount_moderate.wlz

14670_wholemount_weak.wlz

14670_wholemount_possible.wlz

14670_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14670_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8782
Entity Detected:	Mtap4, microtubule-associated protein 4 ( MGI:97178)
Sequence:	sense strand is shown >T8782 CTCTCCCGGCTTCCTGCGGCTCCGGCTTGGGCTCGGCGGCGGCGGCGGGCAGTCGCAGTGGTACAGAATG GCCGACCTCAGTCTTGTGGATGCGTTGACAGAACCACCTCCAGAAATTGAGGGAGAAATAAAGCGAGACT TCATGGCTGCGCTGGAGGCAGAGCCCTATGATGACATCGTGGGAGAAACTGTGGAGAAAACTGAGTTTAT TCCTCTCCTGGATGGTGATGAGAAAACCGGGAACTCAGAGTCCAAAAAGAAACCCTGCTTAGACACTAGC CAGGTTGAAGGTATCCCATCTTCTAAACCAACACTCCTAGCCAATGGTGATCATGGAATGGAGGGGAATA ACACTGCAGGGTCTCCAACTGACTTCCTTGAAGAGAGAGTGGACTATCCGGATTATCAGAGCAGCCAGAA CTGGCCAGAAGATGCAAGCTTTTGTTTCCAGCCTCAGCAAGTGTTAGATACTGACCAGGCTGAGCCCTTT AACGAGCACCGTGATGATGGTTTGGCAGATCTGCTCTTTGTCTCCAGTGGACCCACGAACGCTTCTGCAT TTACAGAGCGAGACAATCCTTCAGAAGACAGTTACGGTATGCTTCCCTGTGACTCATTTGCTTCCACGGC TGTTGTATCTCAGGAGTGGTCTGTGGGAGCCCCAAACTCTCCATGTTCAGAGTCCTGTGTCTCCCCAGAG GTTACTATAGAAACCCTACAGCCAGCAACAGAGCTCTCCAAGGCAGCAGAAGTGGAATCAGTGAAAGAGC AGCTGCCAGCTAAAGCATTGGAAACGATGGCAGAGCAGACCACTGATGTGGTGCACTCTCCATCCACAGA CACAACACCAGGCCCAGACACAGAGGCAGCACTGGCTAAAGACATAGAAGAGATCACCAAGCCAGATGTG ATATTGGCAAATGTCACGCAGCCATCTACTGAATCGGATATGTTCCTGGCCCAGGACATGGAACTACTCA CAGGAACAGAGGCAGCCCACGCTAACAATATCATATTGCCTACAGAACCAGACGAATCTTCAACCAAGGA TGTAGCACCACCTATGGAAGAAGAAATTGTCCCAGGCAATGATACGACATCCCCCAAAGAAACAGAGACA ACACTTCCAATAAAAATGGACTTGGCACCACCTGAGGATGTGTTACTTACCAAAGAAACAGAACTAGCCC CAGCCAAGGGCATGGTTTCACTCTCAGAAATAGAAGAGGCTCTGGCAAAGAATGATGAGTCCTCTGCAGA AATACCTGTGGCTCAGGAGACAGTGGTCTCAGAAACAGAGGTGGTCCTGGCAATAGAAGTGGTACTGCCC TCAGATCCCATAACAACATTGACAAAGGATGTGACACTCCCCTTAGAAGCAGAGAGACCGTTGGTGACGG ACATGACTCCATCTCTGGAAACAGAAATGACCCTAGGCAAAGAGACAGCTCCACCCACAGAAACAAATTT GGGCATGGCCAAAGACATGTCTCCACTCCCAGAATCAGAAGTGACTCTGGGCAAGGACGTGGTTATACTT CCAGAAACAAAGGTGGCTGAGTTTAACAATGTGACTCCACTTTCAGAAGAAGAGGTAACCTCAGTCAAGG ACATGTCTCCGTCTGCAGAAACAGAGGCTCCCCTGGCTAAGAATGCTGATCTGCACTCAGGAACAGAGCT GATTGTGGACAACAGCATGGCTCCAGCCTCCGATCTTGCACTGCCCTTGGAAACAAAAGTAGCAACAGTT CCAATTAAAGACAAAGGAACTGTACAGACTGAAGAAAAACCACGTGAAGACTCCCAGTTAGCATCTATGC AGCACAAGGGACAGTCAACAGTACCTCCTTGCACGGCTTCACCAGAACCAGTCAAAGCTGCAGAACAAAT GTCTACCTTACCAATAGATGCACCTTCTCCATTAGAGAACTTAGAGCAGAAGGAAACGCCTGGCAGCCAG CCTTCTGAGCCTTGCTCAGGAGTATCCCGGCAAGAAGAAGCAAAGGCTGCTGTAGGTGTGACTGGAAATG ACATCACTACCCCGCCAAACAAGGAGCCACCACCAAGCCCAGAAAAGAAAGCAAAGCCTTTGGCCACCAC TCAACCTGCAAAGACTTCAACATCGAAAGCCAAAACACAGCCCACTTCTCTCCCTAAGCAACCAGCTCCC ACCACCTCTGGTGGGTTGAATAAAAAACCCATGAGCCTCGCCTCAGGCTCAGTGCCAGCTGCCCCACACA AACGCCCTGCTGCTGCCACTGCTACTGCCAGGCCTTCCACCCTACCTGCCAGAGACGTGAAGCCAAAGCC AATTACAGAAGCTAAGGTTGCCGAAAAGCGGACCTCTCCATCCAAGCCTTCATCTGCCCCAGCCCTCAAA CCTGGACCTAAAACCACCCCAACCGTTTCAAAAGCCACATCTCCCTCAACTCTTGTTTCCACTGGACCAA GTAGTAGAAGTCCAGCTACAACTCTGCCTAAGAGGCCAACCAGCATCAAGACTGAGGGGAAACCTGCTGA TGTCAAAAGGATGACTGCTAAGTCTGCCTCAGCTGACTTGAGTCGCTCAAAGACCACCTCTGCCAGTTCT GTGAAGAGAAACACCACTCCCACTGGGGCAGCACCCCCAGCAGGGATGACTTCCACTCGAGTCAAGCCCA TGTCTGCACCTAGCCGCTCTTCTGGGGCTCTTTCTGTGGACAAGAAGCCCACTTCCACTAAGCCTAGCTC CTCTGCTCCCAGGGTGAGCCGCCTGGCCACAACTGTTTCTGCCCCTGACCTGAAGAGTGTTCGCTCCAAG GTCGGCTCTACAGAAAACATCAAACACCAGCCTGGAGGAGGCCGGGCCAAAGTAGAGAAAAAAACAGAGG CAGCTACCACAGCTGGGAAGCCTGAACCTAATGCAGTCACTAAAGCAGCCGGCTCCATTGCGAGTGCACA GAAACCGCCTGCTGGGAAAGTCCAGATAGTATCCAAAAAAGTGAGCTACAGCCATATTCAATCCAAGTGT GGTTCCAAGGACAATATTAAGCATGTCCCTGGAGGTGGCAATGTTCAGATTCAGAACAAGAAAGTGGACA TATCCAAGGTCTCCTCCAAGTGTGGGTCCAAAGCTAATATCAAGCACAAGCCTGGTGGAGGAGATGTCAA GATTGAAAGTCAGAAGTTGAACTTCAAGGAGAAGGCCCAAGCCAAAGTGGGATCCCTTGATAACGTGGGC CACTTGCCTGCAGGAGGTGCCGTGAAGACTGAGGGCGGTGGCAGTGAGGCCCTTCCGTGTCCAGGCCCCC CCGCTGGGGAGGAGCCAGTCATCCCTGAGGCTGCGCCTGACGCTGGCGCCCCTACTTCAGCCAGTGGCCT CAGTGGCCACACCACCCTGTCAGGGGGTGGTGACCAAAGGGAGCCCCAGACCTTGGACAGCCAGATCCAG GAGACAAGCATCTAATGATGACATTCTGGTCTCGTCTTCCATCTCCCCTGTGTTCCCTCTCTTGTCTCCC CCCCTTGTTACCCTCTCCCATCCCTCCTCACGTGTCACTGCAGATTGAGACCTACAGGCTGACGTTCCGG GCAAATGCCAGGGCCCGCACCGACCATGGGGCTGACATTGTCTCCCGGCCTCCCCACTTCCCTGGTGGCC CAAGCTTGGGGTCTCGAGCCCTGGGCTCCTTTTCCCGGGCTGTCTACTAGACTTGTAAGCTCTTGGGTGC TGGGCAGCCCCTTTAGGCCTCCCTCCCTCCTGCCTCGCTTGCTCAAGGCAGCAGCAGCCCCACTCGCTGC CATTACACCTCCTTCCACCCCTTTCTGGGCCAACTCCAACTTCACTATCTCACCACTTCACCACTGCACC ACGGAGAAAGTTGGGCGGGGACGGGAGTCCAGTGGGATCTCTGTGGGATCTAGGGGTGAGGAGCTGGATT CCAGCCTTGGCTGTTTGGTTTTTTTGGACCAAACCCAAAAGAAACTGACAAACCTCTCTTCCCTGGCTCA ACCTGGAGGGACTGGTAAAGGTGAACTCCACGTGGAGACCAGCACCGACTGAATCCCTGGAACCTAAACC ACTCCTTGACCCCACGGGGTGGAAGCCACCATTTGGTGGTACCAGGCCCTTTTGCTGCCTCACCTCAAGT TAGTCAAGGATCAGTCCTCCTTGTCCCCACGACTTATCGTACCTGAGTAGCTGCTGTCTCACTTTTTTAT TCTTTCCTCTCCCAATAACCTATGAACTGGTGGTGTAGTTTGCAGGCTGAAGCCATGTCTAAATGAGAGT TCTTGGTAGGTGGTGAGGGAAGTGGGGAAGGAGATCCCGAGTCTCCACTGAGGAGGCTGTCAGATATTGG CGGGGGTTGCCCTACTCTGGGCAGCTTGGGCCCTGCAGGAAGAAGTGAAGTCAGACAGCTAGGGTGGGTC AGTGTGGCTGATTGTGTTGGTATTGTGTGTATGCTGCTTTTCTAACCAAGAGGCTGGTTTTGGCATCTAT CTCATTCCCTGGGAATGCGGTGGTTAAATGTGTGATACAAAATCAGGGCTGGAGAAACAGAAATGAAGGT CAAAATCGCTGGCCTGTACCTACAGGCCATAGCACCCAAATCCTCATATCTAGGGGAGCCTGAAATGTCC AGAACGCTTGTGAACAGTGGGGAATCAGGTGTGAAAATTTAAAACTAAAATGCATGAGACTGAGGCCATC CAGTCTTCTGGTGTGGCGGGAGAGGTGGAGCCATGGTGGTGCAGCCATCCCAGCGTGGTCTGCTCATGGA TGGGATGGTTGCAATGTTGAGAAAATCCCGGGAATTTTATCAACACAAGATTCTTATTGCACTTGTATTT TTTGTATTAAAGTTTGCATGGTTTCTAATAAAGGATTCAAACCTAAGTTTGTATTGAAATGGCCTGGGAG TGTCTAAGGGCTCCTCTGATAGGGCATTTTCTGCTGGGCAGATTTGCCTCTCAGGAGGCTGGCCCAGACT TGGTCCCCTTGGCCCTCCTCCGAACAGACTCTGCCTTTGCTTGGGTACAGTTTGAGGAGGTAGAGGTCTT TACTCTCTCTCTGCCAAGGGAAGGTATCCCTGGGCTTGAAGGTCTTCTCCATTTATGAGGTAGCAGTTAC TTTGTGATAATCTTGACACTGAATCCCTAGCTCTCTAAGCACACACAAAGAATTGCTGGGACCTGTGGAA AAAAGACTGCCTGAGCTGGGGAGTGGCTTTCTTCCCAAGCTGTCAGAACTAAGCTTGGAGCCTGCATTGT CTGTATTTTGCAGCAATAGGGGCCCACGGAGCTGGGAAGGACACAGTGGGGACCAGGGTAGTGATGACTG AGCGTATACAGGTACTTGCTACCCTCCTTGATGTTGATCTTAGGGCCTTGAAGATGCTAAGGAAATTGAC AAGAAACAAGTCCATTGGCCAAGGATTAGAGAAAGGAAGGTAACCCAAGCTCTGTAGGTAGCTCCCTGGA AGGATCCCACGAGCCAGAGAACCTGAGAAGGCTGGTCTAGCAGCTTGGGACCACAGGCCATGTGATGTGC ATACTTCAAAATGTTTGGGAATAAGGTCCTTCTGTATTTTATAGTTTGCTCATGGCTTAGCCATTACCCA TGTCTGTAATTGTTGTACTGATTTAAACAATAAAGCTGCCTGACATCCCAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6407218 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6407218 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14673 same embryo
	EMAGE:14672 same embryo
	EMAGE:14671 same embryo
	EMAGE:14674 same embryo
	EurExpress:euxassay_005584 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source