EMAGE mouse gene expression spatial database

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EMAGE:14673

Dffb DNA fragmentation factor, beta subunit ( MGI:1196287)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005564. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005564_01	euxassay_005564_02	euxassay_005564_03	euxassay_005564_04

euxassay_005564_05	euxassay_005564_06	euxassay_005564_07	euxassay_005564_08	euxassay_005564_09

euxassay_005564_10	euxassay_005564_11	euxassay_005564_12	euxassay_005564_13	euxassay_005564_14

euxassay_005564_15	euxassay_005564_16	euxassay_005564_17	euxassay_005564_18	euxassay_005564_19

euxassay_005564_20	euxassay_005564_21	euxassay_005564_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14673_wholemount_strong.wlz

14673_wholemount_moderate.wlz

14673_wholemount_weak.wlz

14673_wholemount_possible.wlz

14673_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14673_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8719
Entity Detected:	Dffb, DNA fragmentation factor, beta subunit ( MGI:1196287)
Sequence:	sense strand is shown >T8719 GCTACGGTACCGCAGCCCTGCAATGTGCGCGGTGCTCCGCCAACCCAAATGCGTCAAGTTGCGAGCCCTA CATAGCGCCTGCAAGTTCGGCGTGGCGGCCCGGAGCTGCCAGGAGCTGCTGCGTAAGGGCTGCGTCCGCT TCCAGCTCCCGATGCCCGGTTCCCGGCTGTGCCTGTACGAAGATGGCACGGAGGTGACGGACGACTGCTT CCCGGGCCTTCCCAACGACGCTGAGCTCCTATTGCTCACCGCTGGCGAGACCTGGCATGGCTATGTGAGT GACATCACACGTTTCCTCAGTGTGTTTAATGAGCCACATGCCGGCGTCATCCAGGCTGCACGGCAACTGC TGTCAGATGAGCAGGCCCCACTGAGGCAAAAGCTGCTGGCCGATCTTCTGCATCACGTGAGCCAGAATAT TACTGCAGAGACCCGGGAGCAGGACCCATCCTGGTTTGAAGGTTTGGAGTCGAGATTCAGGAATAAGTCG GGCTATCTGAGATACAGCTGTGAGAGTCGGATCCGGGGTTACCTAAGAGAGGTGAGCGCTTACACCTCTA TGGTGGATGAAGCAGCTCAAGAAGAGTACCTGCGAGTCCTTGGCTCCATGTGCCAGAAGCTCAAATCGGT GCAGTACAATGGCAGCTATTTCGACAGAGGTGCAGAGGCCAGCAGCCGCCTCTGTACTCCAGAAGGATGG TTCTCCTGCCAGGGCCCCTTTGACCTGGAGAGCTGTCTTTCCAAGCACTCCATCAACCCCTATGGCAACA GAGAGAGCCGGATCCTCTTCAGTACCTGGAACCTGGATCATATAATAGAGAAGAAGCGCACCGTGGTACC CACGCTGGCTGAAGCCATCCAGGATGGGAGGGAGGTGAACTGGGAGTACTTCTACAGCCTGCTCTTCACT GCCGAGAACCTGAAGCTGGTGCACATCGCCTGCCACAAGAAGACACACAAGCTGGAGTGCGACCGCAGTA GGATCTATCGGCCTCAGACAGGATCCAGGAGGAAGCAGCCTGCTCGGAAGAAGCGCCCTGCTCGGAAGCG CTAGTGACACAGCCCAGTGTGCAGAGGACGGTCAGTGCCGGTGCTCATCGTGTTCCTGTGGCAGGGTCTT CACGCTCACCGCCCCAGGGCTGAACTTGGCCACTGTGATGTGTGTGGGGTGCTAGTTTGTGCTGAGCTTT TATGGCTTCTATTTCTACTGCAAATGGCAGCTCTGACTATACGCCTCTGAAAGAAACGGTGCTTCTGGGA TAAATGTTTGCATTTTATAACGAACATGCAGGACATCCTCTGCTGCATTGTTTAAAATGGCAGAAGGTGG GAAGCCATCCAGATGTCTCCTGGTGTGAACAGCCAACTGTGGCATTATTTTGAGCCATGTGAAACTGATG TTTTTAATGGGTTAAATAATGGCTGTCATGAATTTCATGACTCAACACTGATATATGTATGTACGCACGA TTGGAGCAGGTTTAAACTTTCATGTGGAGAAATAGGGAACTGTTAAAGGCATAGATGGCTTTATCTGTGC AGGGAATGACCTTGAACTCATGATCTTCCTGCCTCATCCCTAGAGCTGGGACCACAGGTGTGCACCACCA CACCTGGCATTTGGTTTTGTAAATGTGTGTCAGCTTGTGCATGGTCTGAGGAAATGACACTCTGTAACCC TGGGGGATTCAGCAGGGCTGAGATTGATCACCAAGTACAATCTCCTACATCATGTATTTTATAATACTTC ATTCTGCTTTTTCTCTGAGACCGGGTCTCACTATGTAGCTCTGGCTGGACTGGAGTTCACTATATAGACC AGCCAACCTCTGCCTCCCGAGTGCTGGGATTAAAGGTGTGTGCCACCACGCCCAACCTGAGTTTATTTTT GAAATGGGCATTTTATATATTTTGTAATGTTTGGATTTTTATATTTGCCATGTAATCAGAAAAACAATAA AGTGTTTCTAAAATAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6403143 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6403143 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14672 same embryo
	EMAGE:14671 same embryo
	EMAGE:14670 same embryo
	EMAGE:14674 same embryo
	EurExpress:euxassay_005564 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source