EMAGE mouse gene expression spatial database

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EMAGE:14674

Rad23b RAD23b homolog (S. cerevisiae) ( MGI:105128)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005600. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005600_01	euxassay_005600_02	euxassay_005600_03	euxassay_005600_04

euxassay_005600_05	euxassay_005600_06	euxassay_005600_07	euxassay_005600_08	euxassay_005600_09

euxassay_005600_10	euxassay_005600_11	euxassay_005600_12	euxassay_005600_13	euxassay_005600_14

euxassay_005600_15	euxassay_005600_16	euxassay_005600_17	euxassay_005600_18	euxassay_005600_19

euxassay_005600_20	euxassay_005600_21	euxassay_005600_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14674_wholemount_strong.wlz

14674_wholemount_moderate.wlz

14674_wholemount_weak.wlz

14674_wholemount_possible.wlz

14674_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14674_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T4112
Entity Detected:	Rad23b, RAD23b homolog (S. cerevisiae) ( MGI:105128)
Sequence:	sense strand is shown >T4112 CCACGCGTCCGCGGCGAGTCACGGTGATGGCGGCCACCATCCTGTGGTGAGGTAGCGGGTTCTCTGCGTC TCTCGGGGAGTCCCTCGCGCCTCCTGCGGGCCCAGTGGCTGGCGCGTGAGGAAGCACCGCGGGTCGAGCT TACCGGGAAAGCCGCGGGAGCGAGCGGCGGCGCGGCCAGGGGAGACGCGGGGCCCGGGGTGAGTGCGACT CCGGGCGAATGTGACAAGCCCCCCCACCCCCACCGCCTTCCTCCCGAGAGCGCGAGGATCGCGGCCACCC CGGGGCGGCCAGGCCCCGGCTTAGGCACCATGCAGGTCACCCTGAAGACCCTTCAGCAGCAGACCTTCAA GATCGACATCGACCCGGAGGAGACGGTAAAGGCATTGAAAGAGAAGATTGAATCTGAAAAGGGGAAAGAT GCCTTTCCGGTAGCAGGTCAGAAGTTAATTTATGCAGGCAAAATCCTCAGTGATGATACTGCTCTCAAAG AATATAAAATTGATGAGAAAAACTTTGTGGTGGTTATGGTGACAAAACCCAAAGCAGTGACAACAGCAGT GCCAGCTACAACCCAGCCATCAAGTACTCCCAGCCCCACTACAGTCAGTTCTTCCCCAGCAGTGGCTGCG GCCCAGGCTCCAGCTCCCACCCCTGCTCTGGCTCCCACTTCCACTCCTGCCTCCACTACTCCAGCCTCCA CTACAGCCTCTTCTGAACCCGCACCTGCTGGTGCCACTCAGCCTGAGAAACCTGCAGAAAAGCCAGCCCA GACACCAGTGCTTACTAGCCCAGCACCAGCTGACAGTACACCAGGAGATTCTTCCCGGTCAAATCTTTTT GAAGATGCAACAAGTGCCCTTGTGACAGGTCAGTCTTATGAGAATATGGTAACTGAGATCATGTCAATGG GCTATGAACGAGAACAAGTAATTGCAGCCCTGAGAGCCAGCTTCAACAACCCTGACAGAGCTGTGGAATA TCTTCTAATGGGAATCCCTGGAGATAGAGAAAGTCAGGCTGTGGTTGACCCTCCTCCTCAGGCTGTGAGT ACTGGAACTCCTCAGTCTCCAGCAGTAGCTGCAGCTGCAGCAACCACGACAGCAACTACAACAACCACTT CTGGAGGCCACCCCCTTGAATTTTTACGGAATCAGCCTCAGTTTCAACAGATGAGACAAATTATCCAGCA GAATCCTTCTTTGCTGCCAGCCTTGCTACAGCAGATAGGTCGGGAGAACCCTCAGCTGCTGCAGCAAATT AGCCAACACCAGGAGCATTTTATTCAGATGCTGAATGAACCAGTTCAGGAAGCAGGCGGTCAAGGCGGAG GAGGCGGTGGCGGAGGAGGCGGTGGCGGAGGAGGCGGTGGAGGAATTGCAGAAGCTGGAAGTGGGCACAT GAATTACATTCAAGTAACACCTCAGGAGAAAGAAGCTATAGAACGGTTAAAGGCATTAGGATTTCCTGAA GGACTTGTGATTCAAGCATATTTTGCTTGTGAGAAGAATGAGAATCTGGCTGCCAACTTTCTTCTACAGC AGAACTTTGATGAAGACTGAAGGGACTTTTTTGTACCTCACACTCACACCAGTGCATTACACTAACCTTG TTCACTGGATTGTCTGGGGTGACTTGGGCTCATATCCACAATATTTGGTATACGGTAGTAGGTTGGTGGG GGTGGGGGTGGGGAGCTAGGATATAGGGCAGGGATACATACAGTTGCATGTCTGCTTCAATTAGCAGATG CTGCAGATCCACACAGTGTAAAATACTATACAACCAAACATCCGCCTTTACAGGTCGTTATTCTTCTAAG CAGTAGGTAACTTTTCCTGGGTTTCATTCTTTCTAGTGTACTAGATCCAGAAATTTAGTGTGATGCCCTG CTTTCTCTTTTCTTTGACTTAACATTGGTTTCTGAAAGAATCTTTGCTACCTAGAACCTGCAGTCTGTTT TATGGCAACACTGGATAATGGCTTTTTGAAATTGAGGGGGAAAAATTGGAGCATCTGTAAACAGAAATGC CAAATTATTGATAGTCATCGTTGCTGCTTCACATTAGTATGAGACTGATGCAGAAGGAAGCCCATTCTTC GTGTTCAGTGCTGGGGGTGGGGTGGGGACTTTTCTTAAAGCGAAAATAATTACTGCTATTTTAAAATGTC TTGATTATTGAATGTGAGACCCTCTGACATGATTTGAGAAGCTGCACACATAGGCAGAGTCATTTTCCTG TTTACATTCTTTGTTTGTTTGGGGAAGATTGGTAGGGGTCTAATTACTGTTCACTTCCTTGTTGTATTGC AGTAAAAGATTTAAAACAACCATTGCATGTTTGCTTTTGATGTATCCCTTTGTGAAATCAGCACTTCTGG GGCCAATGAAAAAACTGTGCAGCATTCACTCCCCTCCCCCCTCCCCTCCCCTCCCCTCCCCTCCCCTCCG TAGAGATGTGTTTGATCAGCAAGTCAGGAGTCAAACTGCTGCCTTTAAAAAAACACACAAAAAGCTGATT CAGTTCAAAATTAATGCAAATCTTTCAAAACTGGGTTTCTGATACTTGTGGATGTTTTTCTTCATTAGAT AAGAATGTATTACCATTAAATCCATTAGTATTACATTGCTTTCAAAGAGAAGGTGGGTGGAACTATAATT CAGCATCTTTTACTGCATTTAAAGATGGGAGCAAACTTTTGTATGGTTGGGAGATGTGGCTGGAAAGTAC TTTGGAAAATATACAATCAAGATATTTCGTCTCATATTAAAAGAGAAATCTCAATCACAAAAAAAAAAAA AAA
Notes:	The probe template was PCR amplified from IMAGE:4459657 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4459657 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14673 same embryo
	EMAGE:14672 same embryo
	EMAGE:14671 same embryo
	EMAGE:14670 same embryo
	EurExpress:euxassay_005600 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source