EMAGE mouse gene expression spatial database

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EMAGE:14701

Gfpt2 glutamine fructose-6-phosphate transaminase 2 ( MGI:1338883)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005728. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005728_01	euxassay_005728_02	euxassay_005728_03	euxassay_005728_04

euxassay_005728_05	euxassay_005728_06	euxassay_005728_07	euxassay_005728_08	euxassay_005728_09

euxassay_005728_10	euxassay_005728_11	euxassay_005728_12	euxassay_005728_13	euxassay_005728_14

euxassay_005728_15	euxassay_005728_16	euxassay_005728_17	euxassay_005728_18	euxassay_005728_19

euxassay_005728_20	euxassay_005728_21	euxassay_005728_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14701_wholemount_strong.wlz

14701_wholemount_moderate.wlz

14701_wholemount_weak.wlz

14701_wholemount_possible.wlz

14701_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14701_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

naris

moderate

regional

moderate expression: see section 10 11 12 weak expression: see section 13

viscerocranium

moderate

regional

Expression in the turbinate bone.

mandible

moderate

regional

moderate expression: see section 11 12

temporal bone petrous part

moderate

regional

moderate expression: see section 01 03 04 07 08 09 19 22 weak expression: see section 02 14 15 16 18 20 21

sternum

moderate

regional

moderate expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7285
Entity Detected:	Gfpt2, glutamine fructose-6-phosphate transaminase 2 ( MGI:1338883)
Sequence:	sense strand is shown >T7285 AGCGCGAGGCGCAGCGAGTCGTCTGAGCGTCTGGAGCGATCGGAGCCACCATGTGCGGAATCTTTGCCTA CATGAATTACAGAGTTCCCAAGACAAGGAAAGAGATTTTCGAAACCCTTATCAGGGGTCTGCAGCGGCTG GAGTACCGGGGCTATGACTCTGCGGGGGTTGCCATTGATGGGAATAACCACGAAGTCAAAGAAAGACACA TCCATCTTGTGAAGAAAAGGGGGAAAGTAAAGGCTCTGGATGAAGAACTTTACAAGCAAGATAGCATGGA CTTGAAGGTGGAGTTTGAGACACACTTCGGCATTGCCCACACACGTTGGGCCACCCACGGGGTTCCCAAT GCTGTCAACAGTCACCCGCAGCGTTCGGACAAAGACAATGAATTTGTTGTCATCCACAACGGGATCATCA CTAATTACAAGGATCTAAGGAAGTTTCTGGAAAGCAAAGGCTACGAGTTTGAGTCAGAAACAGACACGGA GACCATCGCCAAGCTGATTAAATATGTATTTGACAACAGAGAGACTGAGGACATAACGTTTTCCACATTG GTCGAAAGAGTCATTCAGCAGTTGGAAGGCGCCTTTGCACTGGTTTTCAAGAGTATTCACTACCCGGGAG AAGCTGTCGCCACGAGGAGAGGCAGCCCCTTGCTCATCGGGGTACGAAGCAAATACAAACTCTCCACAGA GCAGATCCCCGTCTTATATCCGACATGCAATATCGAGAATGTGAAGAATATCTGCAAGACTAGGATGAAG AGACTGGACAGCTCCACCTGCCTGCACGCTGTGGGCGATAAAGCTGTGGAATTCTTCTTTGCTTCTGATG CAAGTGCCATCATAGAACACACCAACCGGGTCATCTTCTTAGAAGATGATGATATCGCTGCAGTGGCTGA TGGGAAACTCTCCATTCACCGAGTCAAGCGCTCAGCTACTGATGACCCCTCCCGAGCCATCCAGACCTTG CAGATGGAACTGCAGCAAATAATGAAAGGTAACTTCAGCGCATTTATGCAGAAGGAGATCTTCGAGCAGC CAGAATCAGTTTTTAATACCATGAGAGGTCGGGTGAATTTTGAGACCAACACAGTGCTCCTGGGTGGCTT GAAGGACCATTTGAAAGAGATCCGACGATGCCGAAGGCTCATTGTGATTGGCTGTGGAACCAGCTACCAT GCCGCTGTGGCTACACGGCAAGTCTTAGAGGAACTGACCGAGCTGCCTGTGATGGTTGAACTTGCCAGTG ACTTTCTGGACAGGAACACACCTGTGTTCAGGGATGACGTTTGCTTTTTCATAAGCCAATCAGGTGAGAC TGCAGACACGCTCCTGGCGCTGCGATACTGTAAGGATCGAGGTGCGCTGACCGTGGGCATCACCAACACC GTGGGTAGCTCCATCTCCCGGGAGACTGACTGTGGCGTCCACATCAACGCAGGGCCCGAGATTGGGGTGG CCAGCACCAAGGCGTACACCAGCCAGTTCATCTCTCTGGTGATGTTTGGTTTGATGATGTCTGAAGATCG AATTTCTCTACAGAACAGGAGACAAGAGATCATCCGTGGCCTCAGATCTTTACCGGAGCTGATCAAAGAA GTGCTGTCCCTGGATGAGAAGATCCATGACTTGGCCCTGGAGCTCTACACACAAAGGTCTCTCCTCGTGA TGGGACGGGGATATAACTATGCCACATGTCTGGAAGGTGCCTTGAAAATTAAGGAGATAACCTACATGCA TTCAGAAGGTATCCTAGCCGGAGAGCTGAAGCACGGGCCCCTTGCTCTCGTCGACAAGCAGATGCCAGTC ATCATGGTCATCATGAAGGATCCTTGCTTTGCCAAGTGCCAGAATGCCCTGCAGCAGGTCACTGCCCGCC AGGGTCGCCCAATCATACTGTGTTCCAAGGATGACACCGAGAGCTCCAAGTTTGCATATAAAACCATTGA ACTTCCCCACACAGTGGACTGTCTCCAGGGTATCCTGAGCGTGATTCCACTCCAGCTTCTGTCCTTCCAC CTGGCTGTCCTCCGAGGTTATGATGTTGACTTCCCCAGAAACCTAGCCAAGTCTGTCACTGTGGAATGAG AACAGGACTTCTATCACAAGACTGTCGTCACCTGCAGTCTGATTCAAGACCTGAACCAACTGCCAAGATG CCACACGGGAAAGGAAGTGGGCCTCCGTGGGCCCCTCATGCTTCCAGTAGAGCTTGACAGCTTTGACGTG CCTTGTACCCAAAGTGCTTTGCTTACAGCAAATGCTGTTTCTCTGTGTCCTGACGTCACTGAGAAGGGAT TGTTTACACATGGGGATCAGAGCAGACTTTCCACTACTGTGCAATAGAGATACCGCTCTCTTCAGAGTAA CTGTGAACCTTTTTTTAACCAACACTAGCTAGTTTTAAAAGTCGAAATATTTATAATGACGATTGTATAG CTTTTAAGTTATTTTTCTAATGTGTGGCTTTCTGTAGCCATGGTAACGCCCTAGCCCATGCCTCTGGACT ACCCAGAGTGCTGTCTCTGGACACACTCGTGCAGGTGTCATTCATCTCGGTTTGGGGCACTGGCATGAGC CCTTTGTCTCCTTTCTCTTTGTTCTCCCTCCACAAGCTGCTCCTGATTCCTGTCCCCTGAAATCAACGCG TCCCCTCTCCCCTCTCCCTATGTTTCCTTTGAGCCCAGTTAAAGTCTCTCCTCCTTTCAATATTCTCAGA TGGACTCAAATTCCTTAGCTCTCTGACTTCTGCTTAATCAGATTATGGTTTTTTTCTTAAGCCCAGGCAG CACTTTGGTCCCGGCTCCCTGTTCATAGTAAACCAGCCTGAGATGCCCCACACAAGAGGGCGGTTTCGTG GGGCTGCCCTGCTCTATTTAAAAGCGTGCACAATCAATGTACTATGCAATTTTACAATAAAGACAGTGCA ACTTTTTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4167189 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4167189 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14700 same embryo
	EMAGE:14699 same embryo
	EMAGE:14698 same embryo
	EurExpress:euxassay_005728 same experiment
	MGI:4825046 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source