EMAGE mouse gene expression spatial database

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EMAGE:14703

Igsf1 immunoglobulin superfamily, member 1 ( MGI:2147913)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005659. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005659_01	euxassay_005659_02	euxassay_005659_03	euxassay_005659_04

euxassay_005659_05	euxassay_005659_06	euxassay_005659_07	euxassay_005659_08	euxassay_005659_09

euxassay_005659_10	euxassay_005659_11	euxassay_005659_12	euxassay_005659_13	euxassay_005659_14

euxassay_005659_15	euxassay_005659_16	euxassay_005659_17	euxassay_005659_18	euxassay_005659_19

euxassay_005659_20	euxassay_005659_21	euxassay_005659_22	euxassay_005659_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14703_wholemount_strong.wlz

14703_wholemount_moderate.wlz

14703_wholemount_weak.wlz

14703_wholemount_possible.wlz

14703_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14703_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 10 11 weak expression: see section 09 12 16

adenohypophysis

strong

regional

strong expression: see section 13 moderate expression: see section 11 12 14

choroid invagination

weak

regional

weak expression: see section 12 13 14 15 16 17 18

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T1036
Entity Detected:	Igsf1, immunoglobulin superfamily, member 1 ( MGI:2147913)
Sequence:	sense strand is shown >T1036 GTTGGCCTACTGGTTTTTTCTGGTCTCGCTAGAGAAGCCTGGTTGTTGGGAACAGCTCAAGGGGTCGCCA TGCTCTTCATACTCATGGCCCTTCTTTGCTGTGGACTGTGCAATGGGGCATTGACAGAGGAGATTGAAAT AGTCATGCCAACCCCTAAGCCTGAACTGTGGGCAGAGACGAACTTCCCTCTGGCCCCGTGGAAGAACTTA ACCCTCTGGTGCAGAAGCCCATCTGGCTCAACTAAGGAGTTTGTGTTACTGAAGGACGGGACCGGGTGGA TTGCAACCCGCCCGGCCTCAGAGCAGGTCCGGGCTGCCTTCCCTCTTGGCGCCCTGACCCACAGCCACAC CGGGAGTTACCATTGCCATTCATGGGAGGAGATGGCTGTGTCGGAGCCTAGTGAAGCGCTTGAACTAGTG GGGACAGACATCCTTCCCAAACCTGTCATTTCGGCTTCCCTTCCAATCCGGGGCCAGGAACTGCAGATCC GCTGTAAAGGATGGCTGGAAGGTTTGGGGTTTGCTCTGTATAAGAAGGGAGAGCAGGAACCTGTCCAGCA ACTTGGTGCTGTT
Notes:	The probe template was PCR amplified from IMAGE:2088041 using vector specific primers. Forward Primer - name:T7-pME18S-FL3-fw, sequence:CGTAATACGACTCACTATAGGGCCTTCTGCTCTAAAAGCTGCG; Reverse Primer - name:T3-pME18S-FL3-rv, sequence:CAAATTAACCCTCACTAAAGGGCGACCTGCAGCTCGAGCAC. Anti-sense probe was then transcribed from the PCR amplified template using T3 polymerase. EMAGE Editor's Note: the partial probe sequence indicated here was given by the EURExpress Consortium and has been checked using BLAST comparison against all available partial insert sequences of IMAGE:2088041 from NCBI. In cases where no BLAST hit was found (because the two sequence reads are found at opposing ends of the insert sequence), both end sequences were then checked against the appropriate cDNA RefSeq to ensure validity of the information.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14706 same embryo
	EMAGE:14704 same embryo
	EMAGE:14705 same embryo
	EMAGE:14702 same embryo
	EurExpress:euxassay_005659 same experiment
	MGI:4825543 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source