EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14710

Dnmt3a DNA methyltransferase 3A ( MGI:1261827)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005654. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005654_01	euxassay_005654_02	euxassay_005654_03	euxassay_005654_04

euxassay_005654_05	euxassay_005654_06	euxassay_005654_07	euxassay_005654_08	euxassay_005654_09

euxassay_005654_10	euxassay_005654_11	euxassay_005654_12	euxassay_005654_13	euxassay_005654_14

euxassay_005654_15	euxassay_005654_16	euxassay_005654_17	euxassay_005654_18	euxassay_005654_19

euxassay_005654_20	euxassay_005654_21	euxassay_005654_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14710_wholemount_strong.wlz

14710_wholemount_moderate.wlz

14710_wholemount_weak.wlz

14710_wholemount_possible.wlz

14710_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14710_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T6089
Entity Detected:	Dnmt3a, DNA methyltransferase 3A ( MGI:1261827)
Sequence:	sense strand is shown >T6089 CCCACGCGTCCGCCCACGCGTCCGCGCCGCGGCAGCAGGGCGAGCCGGGCCGCCCGACCCGCCGGGCCAC ACGGCAGAGCCGCCTGAAGCCCAGCGCTGAGGCTGCACTTTTCCGAGGGCTTGACATCAGGGTCTATGTT TAAGTCTTAGCTCTTGCTTACAAAGACCACGGCAATTCCTTCTCTGAAGCCCTCGCAGCCCCACAGCGCC CTCGCAGCCCCAGCCTGCCGCCTACTGCCCAGCAATGCCCTCCAGCGGCCCCGGGGACACCAGCAGCTCC TCTCTGGAGCGGGAGGATGATCGAAAGGAAGGAGAGGAACAGGAGGAGAACCGTGGCAAGGAAGAGCGCC AGGAGCCCAGCGCCACGGCCCGGAAGGTGGGGAGGCCTGGCCGGAAGCGCAAGCACCCACCGGTGGAAAG CAGTGACACCCCCAAGGACCCAGCAGTGACCACCAAGTCTCAGCCCATGGCCCAGGACTCTGGCCCCTCA GATCTGCTACCCAATGGAGACTTGGAGAAGCGGAGTGAACCCCAACCTGAGGAGGGGAGCCCAGCTGCAG GGCAGAAGGGTGGGGCCCCAGCTGAAGGAGAGGGAACTGAGACCCCACCAGAAGCCTCCAGAGCTGTGGA GAATGGCTGCTGTGTGACCAAGGAAGGCCGTGGAGCCTCTGCAGGAGAGGGCAAAGAACAGAAGCCGACC AACATCGAATCCATGAAAATGGAGGGCTCCCGGGGCCGACTGCGAGGTGGCTTGGGCTGGGAGTCCAGCC TCCGTCAGCGACCCATGCCAAGACTCACCTTCCAGGCAGGGGACCCCTACTACATCAGCAAACGGAAACG GGATGAGTGGCTGGCACGTTGGAAAAGGGAGGCTGAGAAGAAAGCCAAGGTAATTGCAGTAATGAATGCT GTGGAAGAGAACCAGGCCTCTGGAGAGTCTCAGAAGGTGGAGGAGGCCAGCCCTCCTGCTGTGCAGCAGC CCACGGACCCTGCTTCTCCGACTGTGGCCACCACCCCTGAGCCAGTAGGAGGGGATGCTGGGGACAAGAA TGCTACCAAAGCAGCCGACGATGAGCCTGAGTATGAGGATGGCCGGGGCTTTGGCATTGGAGAGCTGGTG TGGGGGAAACTTCGGGGCTTCTCCTGGTGGCCAGGCCGAATTGTGTCTTGGTGGATGACAGGCCGGAGCC GAGCAGCTGAAGGCACTCGCTGGGTCATGTGGTTCGGAGATGGCAAGTTCTCAGTGGTGTGTGTGGAGAA GCTCATGCCGCTGAGCTCCTTCTGCAGTGCATTCCACCAGGCCACCTACAACAAGCAGCCCATGTACCGC AAAGCCATCTACGAAGTCCTCCAGGTGGCCAGCAGCCGTGCCGGGAAGCTGTTTCCAGCTTGCCATGACA GTGATGAAAGTGACAGTGGCAAGGCTGTGGAAGTGCAGAACAAGCAGATGATTGAATGGGCCCTCGGTGG CTTCCAGCCCTCGGGTCCTAAGGGCCTGGAACCACCAGAAGAAGAGAAGAATCCTTACAAGGAAGTTTAC ACCGACATGTGGGTGGAGCCTGAAGCAGCTGCTTACGCCCCACCCCCACCAGCCAAGAAACCCAGAAAGA GCACAACAGAGAAACCTAAGGTCAAGGAGATCATTGATGAGCGCACAAGGGAGCGGCTGGTGTATGAGGT GCGCCAGAAGTGCAGAAACATCGAGGACATTTGTATCTCATGTGGGAGCCTCAATGTCACCCTGGAGCAC CCACTCTTCATTGGAGGCATGTGCCAGAACTGTAAGAACTGCTTCTTGGAGTGTGCTTACCAGTATGACG ACGATGGGTACCAGTCCTATTGCACCATCTGCTGTGGGGGGCGTGAAGTGCTCATGTGTGGGAACAACAA CTGCTGCAGGTGCTTTTGTGTCGAGTGTGTGGATCTCTTGGTGGGGCCAGGAGCTGCTCAGGCAGCCATT AAGGAAGACCCCTGGAACTGCTACATGTGCGGGCATAAGGGCACCTATGGGCTGCTGCGAAGACGGGAAG ACTGGCCTTCTCGACTCCAGATGTTCTTTGCCAATAACCATGACCAGGAATTTGACCCCCCAAAGGTTTA CCCACCTGTGCCAGCTGAGAAGAGGAAGCCCATCCGCGTGCTGTCTCTCTTTGATGGGATTGCTACAGGG CTCCTGGTGCTGAAGGACCTGGGCATCCAAGTGGACCGCTACATTGCCTCCGAGGTGTGTGAGGACTCCA TCACGGTGGGCATGGTGCGGCACCAGGGAAAGATCATGTACGTCGGGGACGTCCGCAGCGTCACACAGAA GCATATCCAGGAGTGGGGCCCATTCGACCTGGTGATTGGAGGCAGTCCCTGCAATGACCTCTCCATTGTC AACCCTGCCCGCAAGGGACTTTATGAGGGTACTGGCCGCCTCTTCTTTGAGTTCTACCGCCTCCTGCATG ATGCGCGGCCCAAGGAGGGAGATGATCGCCCCTTCTTCTGGCTCTTTGAGAATGTGGTGGCCATGGGCGT TAGTGACAAGAGGGACATCTCGCGATTTCTTGAGTCTAACCCCGTGATGATTGACGCCAAAGAAGTGTCT GCTGCACACAGGGCCCGTTACTTCTGGGGTAACCTTCCTGGCATGAACAGGCCTTTGGCATCCACTGTGA ATGATAAGCTGGAGCTGCAAGAGTGTCTGGAGCACGGCAGAATAGCCAAGTTCAGCAAAGTGAGGACCAT TACCACCAGGTCAAACTCTATAAAGCAGGGCAAAGACCAGCATTTCCCCGTCTTCATGAACGAGAAGGAG GACATCCTGTGGTGCACTGAAATGGAAAGGGTGTTTGGCTTCCCCGTCCACTACACAGACGTCTCCAACA TGAGCCGCTTGGCGAGGCAGAGACTGCTGGGCCGATCGTGGAGCGTGCCGGTCATCCGCCACCTCTTCGC TCCGCTGAAGGAATATTTTGCTTGTGTGTAAGGGACATGGGGGCAAACTGAAGTAGTGATGATAAAAAAG TTAAACAAACAAACAAACAAAAAACAAAACAAAACAATAAAACACCAAGAACGAGAGGACGGAGAAAAGT TCAGCACCCAGAAGAGAAAAAGGAATTTAAAGCAAACCACAGAGGAGGAAAACGCCGGAGGGCTTGGCCT TGCAAAAGGGTTGGACATCATCTCCTGAGTTTTCAATGTTAACCTTCAGTCCTATCTAAAAAGCAAAATA GGCCCCTCCCCTTCTTCCCCTCCGGTCCTAGGAGGCGAACTTTTTGTTTTCTACTCTTTTTCAGAGGGGT TTTCTGTTTGTTTGGGTTTTTGTTTCTTGCTGTGACTGAAACAAGAGAGTTATTGCAGCAAAATCAGTAA CAACAAAAAGTAGTAGCCTTGGAGAGGAAAGGGAGAGAGGGAAAATTCTATAAAAACTTAAAATATTGGT TTTTTTTTTTTTCCTTTTCTATATATCTCTTTGGTTGTCTCTAGCCTGATCAGATAGGAGCACAAACAGG AAGAGAATAGAGACCCTCGGAGGCAGAGTCTCCTCTCCCACCCCCGAGCAGTCTCAACAGCACCATTCCT GGTCATGCAAAACAGAACCCAACTAGCAGCAGGGCGCTGAGAGAACACCACACCAGACACTTTTCTACAG TATTTCAGGTGCCTACCACACAGGAAACCTTGAAGAAAACCAGTTTCTAGAAGCCGCTGTTACCTCTTGT TTACAGTTTATATATATATGATAGATATGAGATATATATATATAAAAGGTACTGTTAACTACTGTACATC CCGACTTCATAATGGTGCTTTCAAAACAGCGAGATGAGCAAAGACATCAGCTTCCGCCTGGCCCTCTGTG CAAAGGGTTTCAGCCCAGGATGGGGAGAGGGGAGCAGCTGGAGGGGGTTTTAACAAACTGAAGGATGACC CATATCACCCCCCACCCCTGCCCCATGCCTAGCTTCACCTGCCAAAAAGGGGCTCAGCTGAGGTGGTCGG ACCCTGGGGAAGCTGAGTGTGGAATTTATCCAGACTCGCGTGCAATAACCTTAGAATATGAATCTAAAAT GACTGCCTCAGAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:3492853 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:3492853 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14709 same embryo
	EMAGE:14708 same embryo
	EMAGE:14707 same embryo
	EurExpress:euxassay_005654 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source