EMAGE mouse gene expression spatial database

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EMAGE:14737

Pabpc1 poly(A) binding protein, cytoplasmic 1 ( MGI:1349722)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005676. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005676_01	euxassay_005676_02	euxassay_005676_03	euxassay_005676_04

euxassay_005676_05	euxassay_005676_06	euxassay_005676_07	euxassay_005676_08	euxassay_005676_09

euxassay_005676_10	euxassay_005676_11	euxassay_005676_12	euxassay_005676_13	euxassay_005676_14

euxassay_005676_15	euxassay_005676_16	euxassay_005676_17	euxassay_005676_18	euxassay_005676_19

euxassay_005676_20	euxassay_005676_21	euxassay_005676_22	euxassay_005676_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14737_wholemount_strong.wlz

14737_wholemount_moderate.wlz

14737_wholemount_weak.wlz

14737_wholemount_possible.wlz

14737_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14737_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

strong

ubiquitous

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9052
Entity Detected:	Pabpc1, poly(A) binding protein, cytoplasmic 1 ( MGI:1349722)
Sequence:	sense strand is shown >T9052 CGGCGGTTAGTGCTGAGAGTGCGGAGTGTGTGCTCCGGGCTCGGAACACACATTTATTATTAAAAAATCC AAAAAAAATCTAAAAAATCCTTTGAAAAAAACCCAAAAAAAATTTACAAAAAAATCCGCGTCTCCCCCGC CGGAGACTTTTATTTTTTTTCTTCCTCTTTTATAAAATAACCCGGTGAAGCAGCCGAGACCGACCCGCCC GCCCGCGGCCCCGCAGCTCCAAGCAGGAACCCAGAGACCGAGGCCTTCCTGCCGCCCGGACCCAGCACCG CCAGCCTCGCTCCCCCGGTTCCACGGCCGTTGAGTACGCGTCGATTCCGGTTGAATTTTGTCCCTCTGCG CTCGCCCCCGCTCCCCTCCCCCCGGCTCCGTCCCCCGGCCCCGCACTCGCTCTCCTCCTCTCACGGGAAA GGTCGCGGCCTGCGGCCCCGCGGGCAGCCGTGCCGAGATGAACCCCAGCGCCCCCAGCTACCCCATGGCC TCTCTGTACGTGGGGGACCTGCACCCCGACGTGACCGAGGCGATGCTCTACGAGAAGTTCAGCCCGGCCG GGCCCATCCTCTCCATCCGGGTCTGCAGGGACATGATCACCCGCCGCTCCTTGGGCTACGCGTACGTGAA CTTCCAGCAGCCGGCGGACGCGGAACGTGCTTTGGACACCATGAATTTTGATGTTATAAAGGGCAAGCCA GTACGCATCATGTGGTCTCAGCGTGATCCATCACTTCGCAAAAGTGGAGTAGGCAACATATTCATTAAAA ATTTGGACAAATCCATCGACAATAAAGCACTATATGATACGTTTTCTGCGTTTGGTAACATCCTTTCATG TAAGGTGGTTTGTGATGAAAATGGCTCCAAGGGCTATGGATTTGTACACTTTGAAACACAGGAAGCAGCT GAAAGAGCTATTGAAAAAATGAATGGGATGCTTCTAAATGATCGTAAAGTGTTTGTTGGACGATTTAAAT CTCGGAAGGAACGAGAAGCAGAGCTTGGAGCCAGGGCAAAGGAGTTCACCAATGTTTACATCAAGAACTT TGGAGAAGACATGGATGATGAGCGCCTTAAGGAACTCTTTGGCAAGTTTGGGCCTGCCTTAAGTGTGAAA GTAATGACAGATGAAAGTGGAAAATCCAAAGGATTTGGATTTGTAAGCTTTGAAAGGCATGAAGATGCGC AGAAAGCTGTGGATGAGATGAATGGGAAGGAGCTCAATGGAAAACAGATTTATGTTGGACGAGCTCAGAA AAAAGTGGAACGGCAGACGGAACTTAAGCGCAAATTTGAGCAGATGAAGCAAGATAGGATCACCAGATAT CAGGGTGTGAACCTTTATGTGAAAAATCTTGATGACGGGATTGATGATGAGCGTCTCCGGAAGGAGTTTT CTCCGTTTGGTACAATCACCAGTGCAAAAGTAATGATGGAGGGTGGGCGCAGCAAAGGGTTTGGTTTTGT ATGTTTCTCATCCCCTGAAGAAGCCACTAAAGCAGTTACAGAGATGAATGGTAGAATTGTGGCCACGAAG CCACTGTATGTAGCTTTAGCTCAGCGCAAAGAAGAGCGCCAGGCTCACCTCACTAACCAGTATATGCAGA GGATGGCAAGTGTACGAGCTGTGCCCAACCCCGTGATCAACCCCTACCAGCCAGCACCTCCTTCAGGTTA CTTCATGGCAGCTATCCCACAGACTCAGAACCGTGCTGCATACTATCCTCCTAGCCAAATTGCTCAACTA AGACCAAGTCCTCGCTGGACTGCTCAGGGTGCCAGACCTCATCCATTCCAGAATATGCCCGGTGCTATCC GCCCAGCTGCTCCTAGACCACCATTTAGTACGATGAGACCAGCTTCCTCACAGGTTCCACGAGTCATGTC AACACAGCGTGTTGCTAACACATCAACACAGACAATGGGTCCACGTCCTGCAGCTGCTGCTGCTGCAGCC ACTCCTGCTGTCCGCACCGTTCCCCAGTATAAATATGCTGCGGGAGTCCGCAATCCCCAGCAACATCTTA ATGCACAGCCACAAGTTACCATGCAACAGCCTGCTGTTCATGTGCAAGGTCAAGAACCTTTAACTGCTTC CATGTTGGCATCTGCGCCCCCGCAAGAGCAGAAGCAAATGTTGGGTGAACGGCTGTTTCCTCTTATCCAA GCCATGCACCCTTCTCTTGCTGGTAAAATCACTGGCATGCTGTTGGAGATTGATAACTCAGAATTACTTC ACATGCTCGAGTCTCCAGAGTCTCTCCGCTCAAAGGTTGATGAAGCTGTAGCTGTACTACAAGCCCACCA AGCGAAAGAGGCTGCCCAGAAAGCAGTGAACAGTGCCACTGGTGTTCCAACTGTCTAAATTGATCAGGGA CCACGAACAGAAACTCGTGCTTCACCGAAGAAAAATATCTTAAACATCGAAAAATTTAAATATTATGAAA AAAAACATTGCAAAATATAAAATAAATAAAAAAAGGAAAGGAAACTTTGAACCTTATGTACCGAGCAAAT GCCAGGTCTAGCAAACAGTGCTAGTCCTAGATTACTTGATTTAAAACAACAAAAAAATACAAAAAAATAG TAAAATATAAAAACAAATTAATGTTTTATAGACCCTGGGAAAAGAATTTTCAGCAAAGTACAAAAATTTA AAGCATTCCTTTCTTTAATTTTGTAATTCTTTACTGTGGAATAGCTTGGGACATCAGCTCTGTTTTAAGG AGCAGAACTGATGACTGAGCAAGGAAACGTAATTTGGATTATAAAATTCTTGCTTTAATAAAAATTCCTT AAACAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6816124 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6816124 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14736 same embryo
	EMAGE:14739 same embryo
	EMAGE:14738 same embryo
	EurExpress:euxassay_005676 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source