EMAGE mouse gene expression spatial database

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EMAGE:14744

Zfyve20 zinc finger, FYVE domain containing 20 ( MGI:1925537)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005712. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005712_01	euxassay_005712_02	euxassay_005712_03	euxassay_005712_04

euxassay_005712_05	euxassay_005712_06	euxassay_005712_07	euxassay_005712_08	euxassay_005712_09

euxassay_005712_10	euxassay_005712_11	euxassay_005712_12	euxassay_005712_13	euxassay_005712_14

euxassay_005712_15	euxassay_005712_16	euxassay_005712_17	euxassay_005712_18	euxassay_005712_19

euxassay_005712_20

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14744_wholemount_strong.wlz

14744_wholemount_moderate.wlz

14744_wholemount_weak.wlz

14744_wholemount_possible.wlz

14744_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14744_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8848
Entity Detected:	Zfyve20, zinc finger, FYVE domain containing 20 ( MGI:1925537)
Sequence:	sense strand is shown >T8848 CCGGCAGTCAGAACCCGAGGTGGAAAGGCAGGCTGCCGACTGCCTGTCGGAAGCCGCAGGGAGTGAGAAA GGGTGTCGCAGAGAGCGCGGCGGTGGCTGAGATTTCTTCTGCTGAAGAGAGGAAATCTCATCCAAGGCCA CAATGTCACTTCCCAATTATCCGAGTGCTGCCTGCTCAAGCTAACACTGCCAGAGTCCTGCTCCCTCGGG AGGAGTCAAGCTTACTTGGCATGAGGTTCCTGCTTTCCTGGGGTTGAGAGAAGCACAACTATGGCATCTC TTGATGACCCAGGGGAGGTGAGGGAAGGCTTCCTGTGCCCTTTGTGCCTGAAGGACCTTCAGTCTTTCTA TCAACTTCAGTCACATTATGAGGAAGAACACTTGGAAGATCGTGATGTCAAAGGGCAAATTAAAAATCTT GTTCAGAAGGCTAGGAAAGCAAAGAACAAGTTGTTGAAACGAGAAGGAGATGATCGAGTGGAACCAGGGA CCCAAGGATATGAATCATTCAGTTATGGTGGGGTTGATCCTTACATGTGGGAACCCCAAGAGCTTGGTGC TATGAGAAGCCATCTTTCTGACTTCAAAAAACATCGTGCTGCAAGAATTGACCACTATGTTGTTGAAGTC AATAAATTAATCATCAGGTTGGAAAAGCTTACTGCATTTGACAGAACAAATACTGAGACTTCAAAGATTA GAGCAATAGAAAAGTCCGTGGTGCCTTGGGTCAATGACCAGGATGTCCCCTTCTGTCCAGACTGTGGGAA TAAATTCAGCATCCGGAACCGCCGTCACCACTGCCGCCTCTGTGGGTCTATCATGTGCAAGAAGTGTATG GAGCTCATCGGCCTGCCCTTAGCACATAAGCTCACCAGTGCCAGCAAGGACTCTCTGAGCACCCACACCA GCCCCAGCCAGTCTCCTAACAGTGTCCATGGCTCCCGCCGGGGCAGCATCAGCAGCATGAGCAGTGTCAG CTCAGTCCTAGACGAGAAGGATGATGACCGCATCCGCTGCTGCACACACTGCAAGGACAAGCTGCTCAAG CGAGAGCAGCAGATGGACGAGAAGGAGCACACGCCTGACATCGTGAAGCTCTACGAGAAACTTCGGCTTT GCATGGAGAAAGTTGACCAGAAAGCTCCCGAATACATCAGGATGGCAGCATCTTTAAATGCTGGGGAGAC GACCTACAATCTGGAGCATGCCAATGACCTTCGAGTGGAAGTGCAGAAAGTATATGAGCTAATAGACGCT TTAAGTAAGAAGATCTTAACCTTGGGCTTGAACCAAGACCCTTCACCACATCCAAACACTTTGCGGCTAC AGAGAATGATCAGATACTCAGCTACATTATTTGTGCAGGAAAAGTTACTGGGTTTGATGTCACTGCCAAC CAAAGAACAGTTTGAAGAACTGAAAAAGAAAAGGAAGCAGGACCTGGAGCAGAAGAGGACCGTGGAGAGA CAGGCTGCTCTGGAGTCACGGCGGAAGCTTGAGGAAAGGCAGAGTGGTTTGGCATCTCATACAGCCAATG GGGACGTGAGGTCTCTTCGAGGCATCCCTCCCCCCTTGCGAAAGGCTGAGGGCTGGCTCCCACTGTCAGA AGGTCAGGGACAGAGTGAAGATCCTGACCCTCTCCTCCAGCAGATCTATAACATTACATCGTTCATCAGG CAGGCCAAGGCTGCAGGCCGCACAGATGAGGTGCGCACACTTCAGGAGAACCTGCGGCAGCTGCAGGATG AGTATGACCAGCAGCAGACTGAGAAGGCCATTGAGCTGTCCCGGAAGCAGGCCGAGGAGGAGGAGCTGCA GCGGGAGCAGCTACAGATGCTCCGCAAGCGAGAGCTTGAACGAGAACAGGAGCAGTTCCTGGCAGCATCC CTGCAAACACGGACTCGTGTTCTAGAACTCCGAGAAGTTATACCCTTCCAATTGGAGGCCAGTCGAGGGC CTCACATTGATCTCTCCTATTCCTTGGATCAGGACTCCTCCCCAGTTCAGAGCAGCACAGCTCCTGATAT CCTTACACCTGGCTCAGCTCTAGCACCCATGCACTTGTGGTCTGGGCCCCCAGCCCTTGGCCAGGAAACA CTCCCTCAGAGCACCATGTCACAGCAAAGTGATAAGGCCTCACTCAACCCGTTTGATGAAGACGACCTCT CCAGCCCTACAGAGGGTGCTATCAGCCCTGCTGCTGTAGAGGCTTTCCTGGGCCCTCCAGCTGCTGTCAC CAAAGAATACAATCCTTTTGAGGAAGATGCTGAGGAAGAGGAGGTGGCAGAGTTGGGGGCAGGAAATCCC TTCACCGACCCAGACAGTCCAGCACCCAACCCTTTTGATGAGGATGACGGTCCCAGGCCTGCCAGTCCAG CCGCCCCTGGAAACCCTTTTGAAGAGTGCCCCTCCACCAACCCCTTCGAGGTGGACAGTGATAGTGGCAT GGAGGCCGAGGAACACATCGAGGAGGAGCTGCTCCTGCAGCAAATTGACAACATCAAGGCTTACATCTTT GATGCCAAGCAGTGTGGGCGAATGGACGAGGTTGAGGTTCTAACGGAGAACCTCCGGGAGCTGAAGTGCA CCCTGGCTAAGCAGAAGGGGGCCCCTAATTGACAGCAATGGGCAGGAACTGGGCAGAGTCTTGGGCAGCA GGATATCAAGTGTGAGGTGGGGATGGGCAGGCATCTGAACACACACAGGTTGGGGTGGGAACAGTAGTCT CTTGCTGTGCACTTTGAGACATTTCCACTACAGTTGAGCAAGAACGGGAAAAACAATCGGCATTCATGTG CTGGTTTTTCTGTATTCTTTTTTTAATGAAGTATTCCATTTTCAGGGGACTTTACCATTTTATTTTCAAG ATACAGCTCTGTTGGGCCAAGGCAGAAATGGCTCATGGGCACCTCTTAGTTCATTTTCTGCGGACTGTTG TTGGTCATTATGCAGTATTATAGGGCAGCCCTTGACAGGCCCTTCCTGTAGCCAGGATAGGCAGGGCTCT AGCAGAAGTCTGTCCTGACTTGGTTGTTCCCTTTCAGCACGTCACTTAGGGAGATACTGCTGTGAAGCCC AGTGGGACCAGGGCCTCTTTATGGATGGCAGGAATGTTGCCAGTGTTGTCCCCTCCCAGTTAAAGGAAGT AAAATTCCCAAGTGTGAGGGTAGTGGGCTGGTGGTGTCAGTGTGAGCTTGACTGTGAGATAGTAGTGTTC AACTTTTTGGCGGATGTTATGGTTTGGAGAAGTTTCTACTTATGCAGCTGTCTGATCCCCTGGGAACCTC CTTCCAGGTCTTTGGTAGAATGACCGTTCTGCAGGTCACATTCAAGGGCCTGTTGATTTGGAATTGTGAC TGGGAAGCTTCTGTGGAGTATTAGCAGGTGATGGAACACTTCTGAGTCAGGATGATTCTAGTAACTTCAA GGCCCTCCAGTCAGCCCTCTTCCTTGTGGTTTATCCTGACCTGGGAAACTGGTAAGATGTACTGAGAAAT GGAAATATTTGAGGCACAGCCTGAGACCATTCCACAGCCCTGAAGTACTTGAACTCTTGATAGAAAACAG AAAGGGAAGTTATGTAGCTTAGTACTTTGGTTTACAGGAACAGATCGATTGACAGATGACCTTGTAAGGT TCTTGGTGTGCTCAGCTCTGGGTAATTTCTTTGGTGATGATACTCTTTAGTTAATGGACAATAACTGTCC ACTAGTCACTTTCCCCTCAGCTTTCTCTGGGCCATGCCTTTTGTTGGTTACTGGTGTATAAGCTGAAAGT CTTCTGTCACATTCTGGTGAAGATCCATCTAGGTGGTAAGAATGTTATCTGGGTGCAAAGATCTCTATGT TGGCAGCTTTAATCCCAGTTCATTAGTACATGTTGGGGCTTTGGCTGGATAGAGAGCCCTGTATGGGGCT ATGGGAAGGGCTGGAGAAGGAGGGAGCGTATGAACCCTTTGAGTTCTGCTGACAGGTGTTTACTGGCCTG CTAGTAGATTACCAGCCATTTGCCAGCTCTGTTGGTGTCAAGATGTTCTGGAAGGTAGGCAGCCTGGCAT TTTATGTTACCTGGGGCAGGCTACCTCTTTCTAAAGCCCAGGCTCCAGGCATGCCCACCATATCTGCCCT GCTTGGTCTGCTATTGTGTCATGGACAAAGAGGCAGTGCTAACACAAGAATGAAGCTAAATTCTGGTTTC CACCATTACTTTTCCAGACTCAAATCAGGTGTTCAGGGTGGTACAACTGTAGACAATGCTTTTCTTATAA GACTGGGCTTCTTAATTAGCTGTGAGTGACTCGATGGGCAGTAGTGGCACATGACTTTTATCCCAGCACT TGGGAAGTAGAGGCAGGAATTTCTGAGTTCCAATCCAGCCTGGTCTACAGAGTGAGTTCCAGGACAGCCA GGGCTACACAGAGAAACCCTGTCTCAAAAAACAAACAAATAAATAAATAAACAAAAATAAAAATTGAGAA AAAAATTATCTAGTGACTTATTAAATGTACTTCTTTCTGGAACTCACCCCAGGTAGCAGTCTGACTGAGA GCATGGAGAAAACAAATGCTTTTAGACTGACTGACTATTAAACCGGGTGAACCCAGATCCCAGAGGGTTT CCTGAGTTTCTCATTGAATTCCTCGCTGACCATATTCTGAAAGTCCAACAGAACACGAGAGACTTGTCTG CCCCTTGTCTGGATCAGCTCCCAGAATGCATGCAGGTCAGAGTTGGTTTGCAGAGGGGGTTTGGCCTGTG AGGGAACCACAGTGCCAGCTTCTTACCGTGGGTGAATACTCGATGATTCCCAAGGATGGAGCACGTCCTT GATACGGTGTTGGCAGATCCCTTAGGTGTAGGTCCTGTTCATAGTGGACAGGCTTCTTGCTTATTCCCTT CTAACAACACTAAGGAACTGGAGCCTCATCACTGCACTTACCACATATCATGTCTTTGTCGTCAGACAGT AAAACATCTTTCTAAGTCATCATGAGCCATATTGTGTAGCTATGCCATGCGGTTCAAATTGTGGAATTGG CCTCGGTACGAGGTCAGACTAGAGGAGCTCGGTGTGCTGGTAATCATGATGACTGTCAGCAAGATGGTCA TTGATGCGCATGCACTACACTGGGGCCTTGTATTAGAGGCTTTTATTAAAAACATTAAAATATCATTTAT GTAAGGCTTTTATTAAAAAAATAACATGTTCATGGCTGGAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6412839 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6412839 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14743 same embryo
	EMAGE:14742 same embryo
	EMAGE:14741 same embryo
	EMAGE:14740 same embryo
	EurExpress:euxassay_005712 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source