EMAGE mouse gene expression spatial database

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EMAGE:14746

Pcdhga11 protocadherin gamma subfamily A, 11 ( MGI:1935228)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005808. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005808_01	euxassay_005808_02	euxassay_005808_03	euxassay_005808_04

euxassay_005808_05	euxassay_005808_06	euxassay_005808_07	euxassay_005808_08	euxassay_005808_09

euxassay_005808_10	euxassay_005808_11	euxassay_005808_12	euxassay_005808_13	euxassay_005808_14

euxassay_005808_15	euxassay_005808_16	euxassay_005808_17	euxassay_005808_18	euxassay_005808_19

euxassay_005808_20	euxassay_005808_21	euxassay_005808_22	euxassay_005808_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14746_wholemount_strong.wlz

14746_wholemount_moderate.wlz

14746_wholemount_weak.wlz

14746_wholemount_possible.wlz

14746_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14746_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8785
Entity Detected:	Pcdhga11, protocadherin gamma subfamily A, 11 ( MGI:1935228)
Sequence:	sense strand is shown >T8785 TGGATACTCACGCATCTCAACTCTGGAAGTAAAAACACCTCCGTTCGCTTTGGAATATGTTTTGGATGCG GGATACCTGGAACCTCGTAGACACAAAAGCTGACTCGGAAAAATAAATAAAAATTAAACCGGCGCAAGAC CCTTACGGAATAACGCTCACACCCAGCCATGGCGAATCAGTCTCAGCGCCTGGACCGCCGCGCGCTGATC CCGCTGTGCATTTTCCTGGGGACCTTGCTGACCTTTGGGACAGGACAGATCCGATACTCGGTTCCAGAAG AAACAGATAAAGGCTTCTTCGTAGGCAACATCTCCAAAGACCTGGGGCTGGAGCCCTGGGAGGTCGCGGA ACGCGGAGTCCGCATCATCTCCAGGGGACAATCGCAGCTTTTCTCTCTGAATCCGCGAGGCGGCAGCTTG GTCACTGCAGGTAGGATCGACCGTGAAGAGCTATGTGATACGATGCCCTCCTGCATGCTGAATTTGGAAA TTCTCGTGGAGGAAACCCTGAAGATTTACGGAGTTGAGGTGGAAGTACTGGACATTAACGATAACGCTCC CAGCTTCCGGGAAGAGGAAGTAGAGATCAAAGTCAGCGAGCATACCAGTCCTGGGTCGCGATTTCCTCTT CCTAGCGCTAGGGATCCAGACGCAGGGATGAACTCCCTCCAGAGCTACCAGCTCAGCCCAAGCAGTTATT TCTCCTTGCAGGTGAGAGGCGGGACTGATGGGGACAAGAATCCAGAGCTAGTGCTGGAGGAAGGTCTGGA CCGGGAGAAAGAGGCATCTCACCACCTCCTCCTCACAGCCTTAGATGGGGGAGATCCCGTCCGCAAGGGC ACGGTTCCCATTCGCGTGGTGGTCCTCGACGTCAATGATCACATCCCAAAATTTACACAGCCTGTGTATA GAGTGAGTATTCCTGAAAACCTCAGCTCCGGGACGCGGGTGCTCGTGGTAAATGCCTCAGACCCAGATGA GGGGATCAACGGGGAAGTGATATACTCTTTCCGGAAAATGAAAAGCAAAGCTTCTGAAATATTTCAACTG AATTCTCAAACTGGGGAGGTTCTAGTTGGAAAGCCTCTGGATTTTGAAAAATACAGATTCTATGAGATGG AAATTCAAGGCCAGGATGGTGGAGGGCTGCTTAGCACCACTACGTTGTTGATCACGGTTGAAGATGTGAA TGATAACGCTCCAGAGATAACTGTCACCTCTGCCAGTAACTCAGTGCCGGAAAATTCTCCTTCGGGCACA GTAATTGCTCTTCTAAACGTTCAAGATCAAGATTCTGGAGAAAATGGTCAGGTCTCTTGCTTCATTCCTA GTGGTCTTCCTTTTAAGTTAGAAAAGACGTATGGAAATTATTATAAATTGATAACAAATAGTGAACTGGA CCGGGAGCAGGTGGAGAGCTACAATATTACATTGATCGCCAAAGATCAGGGCAGTCCACCGTTGTCTACA GAAACTCACCTACTGCTCAAAGTGGCAGATGCCAACGACAACCCTCCAGTTTTCTCTAGCTCCTCTTACT TGGCCTACATTCCTGAAAACAACCCCAGAGGCTCTTCTATCTTCTCGGTGACCGCCATTGATAGGGATAG CAGAGAAAATGCCCAGGTCACTTACTCTCTGGCTGAGTACACCATCCAGGGAACACCTCTGTCCTCTTAC GTTTCTATCAATTCAGACACGGGTGTGCTGTATGCCCTGCAATCCTTCGACTATGAGCAATTCCAGACCT TACAACTGGGAGTGACCGCTAGTGATAATGGCGACCCACCATTAAGCAGCAATATTTCGCTGACCCTTTT CGTGCTGGACCAGAATGACAACACTCCAGAGATTTTATACCCCTCTCTCCCTACCGATGGTTCCACTGGT GTGGAATTAGCACCTCGCTCCGCAGAGCCGGGTTATTTAGTGACCAAGGTGGTGGCTGTGGACAGAGACT CCGGGCAGAACGCCTGGCTGTCCTATCGCCTGCTCAAAGCCAGCGAGCCAGGACTCTTCTCCGTGGGGCT GCACACGGGGGAGGTACGCACGGCCCGAGCCATGATGGACAAAGATGCTCTCAAACAGAGTCTCGTGGTA ACTGTGCAAGACCACGGCCAGCCTCCTCTCTCCGCCACTGTCAAGCTCACAGTGGCGGTGGCTGACAGCA TTCCAGAGGTTCTGGAGGACTTGGTTAGTCTGGAGTCTCCCGCCAACCCGGATGAGTCAGGCCTCACCCT CTACCTAGTGGTGGCCATCACTGCAGTCTCCTGCATCTTCCTCATCTTTGTCATGGTACTGCTGGCTCTC AAGCTTAGGCGCTGGCACTCATTACGTCTGCTCCAGGCTGCCAGCGGATTGGCTGGTGTGCCTCCTTCGC ACTTTGTGGGCGTGGACGGGATGCGCGCTTTCCTCCAGACGTATTCCCACGAAGTTTCGCTCACTGCAGA CTCAAGGAAGAGTCACCTCATCTTCCCGCAGCCCAACTATGCAGACACACTCATCAGCCAGGAGAGCTGT GGGAAAAGCGAGCCTCTCCTGATAACTGAAGACTCAACTACAGTTTTAGGCAAATGTGAACCGACAGATA TTCAGCAAGCCCCGCCCAACACTGACTGGCGTTTCTCTCAAGCCCAGAGACCCGGCACGAGCGGATCCCA AAATGGTGATGAAACTGGCACCTGGCCCAACAACCAGTTTGATACAGAGATGCTGCAAGCCATGATCTTG GCCTCTGCCAGCGAAGCTGCTGATGGGAGCTCTACCCTGGGAGGGGGCGCTGGCACCATGGGTCTGAGCG CTCGATACGGACCCCAGTTTACCCTGCAGCACGTGCCTGACTACCGCCAGAACGTGTACATCCCTGGCAG CAATGCCACGCTGACCAATGCCGCTGGCAAACGAGATGGCAAGGCTCCAGCAGGTGGTAATGGCAACAAG AAGAAATCGGGCAAGAAAGAGAAGAAGTAATATGGAGGCCAGGCCTTGAACCACAAGGCAGCCTCCCTCC CCAGCCAGTCCAGCTCGTCCTTACTTGTACCCAGGCCTCAGAATTTCAGGGCTCACCCCAGGATACTGGT AGGAGCCAGGGCCATGCTCCCCGTTGGGAAACAGAAACACGTGCCCAAGCCAACACCCCCTCTCTATACC CTAGGGAGTTGAATATGCAAAGAGAGTTCTGCTGGGACCCCCCTATTCAATCAGTGATTGTACCCACATG GGTAGCAGGGTTTGTGTGTGTGGACATACACACACACACACACACACACACACACAAACCATGCCACACT CCCTTAGTTACAGCTGAACTCCTCCATCTTCCAACCCAGGCAGGCTTATCCATCTCCTGCCGCCCTCTCC CCACCCCACCCACTCCTTCAGTCCCTCTCTTTGAGCAAGGTAGCTGGGCTGTTGGGGTGACCGGTGAGCT AAGCCGGCTCCTAGTTCTGAACGGTAGGGAGAGTATTACAATCTTTTGGCCTCTCCTTCAGTTCAGCTTT CCCCCAAAGCATGGTTTGGTACCAGTCTTCTCTTTTCCTTCCAGAGCCTGAGACCAATGCTTAAGTTTTG GGGGAACAGGCACCCTCCCTCTGGTACTGATGATGCTTGCTGGATTTTGGGAAGGCATTTTGCTACTAAG CCTCTTTTCAATGCCTGGGGACAACTAGTCTTTTGTTTTTCATTGTTTGATGTTCCCACTGCATGCTGTG ACTTCCCTACACCTCAAAGAAGAGACTCCTTTGCATGTCTGGAGACAGTAGGGGGTGGTAAACTAAAGGG AAGTTGAGAGTGTATACTGCTGGGAGTGGGGCAGTGGACAAAACAGCCTGTTAATAGGGTCTTGCAAGGG GCTCTGTATGTACCCAGGGGACTGGCTCAATCCTCACATTCTAGCCATAGACAAACACCAGGCCAGACCC CTCCATTCTGGTTCAGCCTGGGCAGCTTGGGCTGAGCCACCAGGACCAATGGATTTAAGCTGACATTTCA GTCCAAGACGACGACTTCTAAGTGAGTTTAAGACCAGAGAGGAAAGAGGGGCCTCTGTGGGTGCTGGGTA CTCCAGAGGTGCCCTTGGTGGGAGGACCAGTGGTCTTAGCAGGAAGGGGGGCCCAGCAAGGTCATTCTTG GACCCTGGGTCTAGTCCAGTAGCTAGAAAAAGGGACCAAGTGGCCATAAAGTCCCAGCCAATGATGGGGC TTTTCCAGTGGGCCCCTGTAGACCTCAAGCCCCTGGCCTCCACCTTACCAGGTGCCATTTCTTCTCAGAA GGCCACTGCCCAGGCCCCCAGGCCGCCCCCTTGTGGCCAGAGAACGGTTAAAGCCCCCCAGTGCCTCCTT GTGCATAGAACTTCCTCTGCCCTAGCCCTTACAGTAGTGTAGAAGATCCCCCTCCCCCGCTGGTGTAGAA TAGCCAATAGTATAGTGTGGTGTGCTTTTACGTGATGGCGAGTGGGCAGCGGGCGGTGGGCTGTACACAG CCGTCTGTCCTTTGAATCTCAATCTGCCTGCGCGGCCCGTGCTGTGTTTTGTGCTGTGTCCACACGCTGC GGCGACCCCTCCCTGTACTGACTTCTCTATAAAAAGCTTCTCTTCGCATAGTCACGTAGCTCCTGCCCAT CCCCCTTCCGATATCTCTCGCAAGTTTTATACTCTAATATTTATATGGCTTTTTTCTTCGAAAATTAAAA ATAAAAAAATGGTTTCTTCTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAA
Notes:	The probe template was PCR amplified from IMAGE:6406939 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6406939 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14748 same embryo
	EMAGE:14750 same embryo
	EMAGE:14747 same embryo
	EMAGE:14745 same embryo
	EMAGE:14749 same embryo
	EurExpress:euxassay_005808 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source