EMAGE mouse gene expression spatial database

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EMAGE:14747

Slc8a2 solute carrier family 8 (sodium/calcium exchanger), member 2 ( MGI:107996)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005720. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005720_01	euxassay_005720_02	euxassay_005720_03	euxassay_005720_04

euxassay_005720_05	euxassay_005720_06	euxassay_005720_07	euxassay_005720_08	euxassay_005720_09

euxassay_005720_10	euxassay_005720_11	euxassay_005720_12	euxassay_005720_13	euxassay_005720_14

euxassay_005720_15	euxassay_005720_16	euxassay_005720_17	euxassay_005720_18	euxassay_005720_19

euxassay_005720_20	euxassay_005720_21	euxassay_005720_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14747_wholemount_strong.wlz

14747_wholemount_moderate.wlz

14747_wholemount_weak.wlz

14747_wholemount_possible.wlz

14747_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14747_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9268
Entity Detected:	Slc8a2, solute carrier family 8 (sodium/calcium exchanger), member 2 ( MGI:107996)
Sequence:	sense strand is shown >T9268 CTCTCCTCCCTCCTCCCTGCTCTCCCTCAGTCCCCGGCTCCTCTCCCTCTTTCTCCCTTTTCTCCTCGGG CGGAGGCGCCCACCGCGGGGGCCCAGCGGGGACCGGCCCGGCCGCAAGGCAGGACCTCACCACCCCCAAT GGCTCCCTTGGCTTTGATGGGGGTTGTTCTCCTTCTGGGGGTACCCCACTGCTTGGGGGAAGCCACTCCG ACCCCTTCCCTACCGCCTCCCACAGCTAATGACAGCGATGCCAGCCCAGAAGGCTGCCAAGGTTCCTACC GCTGCCAACCAGGTGTGCTGCTGCCTGTGTGGGAACCCGAGGACCCATCGCTGGGCGACAAGGTTGCACG GGCCGTGGTGTACTTTGTGGCCATGGTCTACATGTTCCTGGGTGTGTCTATCATTGCCGATCGATTTATG GCATCCATTGAGGTCATCACATCCAAGGAGAAAGAGATCACCATCACCAAGGCAAATGGGGAGACCAGCG TGGGCACGGTGCGCATCTGGAACGAGACGGTGTCCAACCTTACACTCATGGCCCTGGGCTCCTCAGCGCC TGAGATTCTGTTGACTGTCATCGAGGTCTGTGGCCACAACTTCCAGGCCGGTGAGCTAGGCCCAGGCACC ATCGTGGGCAGTGCCGCCTTCAACATGTTTGTGGTCATTGCTGTTTGTGTGTATGTCATCCCGGCTGGCG AGAGCCGTAAGATCAAGCACCTGAGGGTCTTCTTTGTCACAGCCTCCTGGAGCATCTTTGCCTATGTCTG GCTTTATCTCATTCTAGCAGTTTTCTCCCCAGGTGTAGTCCAGGTGTGGGAGGCACTGCTCACACTGATC TTCTTCCCGGTGTGTGTGGTGTTTGCCTGGATGGCGGACAAGCGACTGCTCTTCTACAAGTACGTGTACA AGCGCTACCGCACCGACCCTCGCAGTGGAATCATCATCGGGGCAGAGGGAGACCCACCCAAGAGCATCGA GCTGGACGGCACATTCGTGGGCACTGAGGTCCCTGGCGAGCTGGGCGCATTGGGCACAGGTCCTGCTGAG GCGCGTGAACTAGATGCCAGCCGGCGTGAGGTCATCCAGATCCTTAAGGACTTGAAGCAGAAGCACCCGG ATAAGGACCTGGAGCAGCTGATGGGCATCGCCAAGTACTATGCACTGCTGCACCAGCAGAAGAGCCGCGC CTTCTACCGCATCCAGGCCACGCGGCTGATGACAGGTGCGGGCAATGTGCTGCGCAGACATGCTGCGGAT GCTGCCCGCAGGCCGGGAGCCACCGATGGTGCCCCCGATGATGAGGACGATGGTGCCAGTCGCATCTTCT TTGAGCCCAGCCTCTATCACTGCCTGGAAAACTGCGGGTCAGTGCTGCTGTCCGTGGCTTGCCAGGGCGG TGAGGGCAACAGCACCTTCTACGTGGACTACCGTACCGAGGACGGTTCTGCAAAGGCAGGCTCCGATTAT GAGTACAGCGAGGGCACGCTGGTGTTCAAGCCCGGGGAGACGCAGAAGGACCTGCGCATCGGGATCATCG ACGACGACATCTTCGAGGAGGATGAGCACTTCTTCGTGAGGCTGCTGAACCTGCGTGTGGGCGATGCTCA GGGCATGTTCGAGCCCGACGGCGGTGGGCGGCCCAAGGGGCGGCTGGTGGCGCCGCTGCTGGCCACTGTC ACCATCCTGGACGACGACCACGCGGGCATCTTCTCCTTCCAGGACCGCCTGCTGCATGTGAGCGAGTGCA TGGGCACTGTGGATGTGCGCGTGGTTCGCAGCTCGGGCGCCCGTGGCACTGTACGCCTCCCCTACCGCAC AGTGGACGGCACGGCCCGTGGCGGTGGTGTACATTACGAGGATGCTTGTGGAGAGCTGGAGTTCGGCGAT GATGAGACCATGAAAACTCTTCAGGTCAAGATAGTGGATGATGAAGAGTATGAGAAGAAGGACAACTTCT TCATTGAGCTGGGCCAGCCCCAGTGGCTTAAGCGAGGCATCTCAGCTCTGCTACTCAACCAAGGGAATGG AGACAAGAAGATAACTGCAGAGCAGGAGGAGGCCCAGAGGATAGCAGAGATGGGCAAGCCAGTTCTTGGG GAGAACAATCGCCTCGAGGTCATCATCGAGGAGTCTTATGACTTTAAGAACACGGTGGATAAACTCATAA AGAAAACGAACCTGGCCTTGGTGATTGGGACTCACTCATGGAGGGAGCAGTTTATAGAGGCAGTTACCGT AAGCGCAGGGGACGAGGAGGAGGATGAGGATGGACCTCGTGAGGAGCGGCTGCCGTCCTGCTTTGACTAC GTGATGCACTTCCTGACGGTATTCTGGAAAGTTCTATTCGCCTGCGTTCCACCCACGGAGTACTGCAATG GCTGGGCCTGCTTTGGTGTCTGCATCCTGGTCATTGGTGTGCTCACTGCCCTCATCGGAGACCTGGCCTC ACACTTTGGGTGCACCGTGGGCCTCAAGGACTCAGTCAACGCCGTGGTCTTCGTAGCCCTGGGCACCTCC ATCCCTGACACGTTCGCCAGCAAGGTGGCCGCGCTGCAGGACCAGTGTGCCGACGCGTCCATCGGTAACG TGACCGGCTCCAATGCGGTGAACGTGTTCCTGGGCCTGGGTGTGGCCTGGTCGGTGGCAGCGGTATACTG GGCGGTGCAGGGTCGCCCCTTCGAGGTGCGTGCAGGCACGCTGGCCTTCTCGGTCACGCTGTTCACCGTC TTCGCCTTCGTGTGCATCGCAGTGCTGTTGTACCGGCGCCGGCCGCAGATTGGCGGCGAGCTGGGCGGCC CGCGGGGACCCAAGCTGGCCACCACCGCTCTCTTCCTGGGCCTCTGGTTCCTCTACATTCTCTTCTCCAG CCTGGAGGCTTACTGCCACATCCGGGGCTTCTAGGGCCCTAGCCACCGCCCTGAGGGTGGGGACTTGGTG GCATTTGCTCTTGGACCCACCAGTCTCTCTACCCAGACTCAGCCTGGGCGCTCCCTAGGCTCTTCCTCCT CAGCCTTCTTCCTGGGATCAGCCTCCCTTTCCCCCTGGAATCCCATCCTTTGCCCTCCCTTCTCAGCTGC ATCCCCTTCAGCCCATCCTTTGTGAGGTAAACCCGAGTCCCCTGGCCAGACACTACTTCTCGGTCTTTCT GGGCACCGAGCCTGATTCTGTCTTCTCGTGTCCCTCCCTCAAGCCACGAACTTCCACCTGTGCGCCCAGG TCTTAGAACATCTCCCTAGGTACCTCAGAGGGAGGCTGTGCCATCCCAGGACGCCATCCATCTCTGCCCT TAACCACCCCAACCCTCAGGGAGCTCCCTTGCAGTCCCCAGGGAAAGGAATAGGGGTTTGTGCGGGGAGC AGGGAGAAAGGCACGCACCTTCCCTGATTTATCCACTCAGAGGCTTGGAGATGGAGGAGGGACGTTTGGA GTATCTGTCCCCCAGGCTTAGCCAGAGGAGGCACATCTTGAACCCACTACTTCTGACATTCCAGCGCCCT ACTCACCCTCCACTACCTCTGAGAGCCTAGCCACGCCTTGGAGGGAGGAGCCTGTGTGTGTATATAGTGT GTTTGGGGAGGGGGGACACGGGAGGGTGCATGTCTTGGGGAAAGGGGCGTTGACAGACTTTCCCTTCTGA GAGGGCAGCAGGACTCACTCAGCCTGAGAACCAGCTTAGTGGATGAGACTGAGACTCAAAGGGAGCCCAC CTGACCTCCCCTGACAGTATTCTAGAAGGGTTAACGAAAGTACTCTAGTCTAGAAGGTTTATTTTGCTCT CAGTGCCAGCCAATCTGGGCAGGACCCTCGAAGAGGAGACCGAGGGTCCCAGAGGACCAATGCTACAAGC CAGCAAATGCTGCCACATCTCTGCCTGTTGGGGGGCGGGGGTGGAGGGGAGGGTGGGAGGGGGGCTGGGT GGGCATTTTTAACTTTGCAGGCCATACATCTGTTGGTAGGCCACCTGCATTTTCTTACTGTTGATGTCTC CTGCCCCAAAAAAGACATCCTTGGGGAACGGGGAGTGCTGCCCACTCCTTGCGACCTTAGGTGACCCTGC CACCCTCACAGCCTTCTGCTTTCCCTAAGTTATTATTTTTGCATTTTCTTGTCCATTTGTGCATCAGACC CATTACCCACGGCCCTTCTCACCCCTTGCTCATCAGCATCACTTGATGTCCCTTCTCTGTGGTTTCTGTA AAAAACTGCCATAAAACTTTGAAATTCTGCCTGAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6838206 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6838206 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14748 same embryo
	EMAGE:14750 same embryo
	EMAGE:14745 same embryo
	EMAGE:14749 same embryo
	EMAGE:14746 same embryo
	EurExpress:euxassay_005720 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source