EMAGE mouse gene expression spatial database

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EMAGE:14748

Galnt7 UDP-N-acetyl-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase 7 ( MGI:1349449)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005715. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005715_01	euxassay_005715_02	euxassay_005715_03	euxassay_005715_04

euxassay_005715_05	euxassay_005715_06	euxassay_005715_07	euxassay_005715_08	euxassay_005715_09

euxassay_005715_10	euxassay_005715_11	euxassay_005715_12	euxassay_005715_13	euxassay_005715_14

euxassay_005715_15	euxassay_005715_16	euxassay_005715_17	euxassay_005715_18	euxassay_005715_19

euxassay_005715_20	euxassay_005715_21	euxassay_005715_22	euxassay_005715_23	euxassay_005715_24

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14748_wholemount_strong.wlz

14748_wholemount_moderate.wlz

14748_wholemount_weak.wlz

14748_wholemount_possible.wlz

14748_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14748_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal gland

moderate

regional

moderate expression: see section 06 07 16 17 weak expression: see section 08 18

submandibular gland primordium

moderate

regional

moderate expression: see section 07 08 09 10 16 17 18 19

diencephalon roof plate

moderate

regional

moderate expression: see section 12 13 14

choroid invagination

moderate

regional

moderate expression: see section 07 08 09 10 11 12 16 17 18

metencephalon part of 4th ventricle choroid plexus

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19

stomach

strong

regional

strong expression: see section 07 08 09 10 moderate expression: see section 02 03 04 05 06 11

rectum

strong

regional

strong expression: see section 14

midgut

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17

metanephros

moderate

regional

moderate expression: see section 06 07 09 16 17 weak expression: see section 08 18

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9244
Entity Detected:	Galnt7, UDP-N-acetyl-alpha-D-galactosamine: polypeptide N-acetylgalactosaminyltransferase 7 ( MGI:1349449)
Sequence:	sense strand is shown >T9244 AGAGCGGTGGCGGCGGCTGCGCCGGGCTGTGTGTCTCTCGCCGCCGGAGGAAGATGAGGCTGAAGATCGG GTTCATCTTACGCAGTTTGCTGGTGGTGGGAAGCTTCCTGGGGCTCGTGGTCCTCTGGTCTTCCCTGTCC TCGCGGCCCGACGACCAGAGCCCACTGAGCAGGATGAGGGAAGACAGAGATGTCAATAACCCCTTGCCTA ACCGAGGAGGCAATGGACTAGCTCCTGGAGATGACAGATTCAAACCTGTGGTACCATGGCCTCATGTTGA AGGAGTAGAAGTGGACTTAGAGTCTATTAGAAGAAAAAACAAAGCCAAAAATGAACAGGAGCGCCATGCT GGAGGAGATTCCCAGAGAGACGTAATGCAGAGGCAGTACCTTACATTTAAGCCTCAGACCTTCACCTACC GTGATCCTGTGCTACGCCCAGGGGTCCTCGGGAACTTTGAACCCAAAGAACCCGAGCCTCACGGAGTGGT TGGTGGCCCTGGAGAGAAAGCCAAGCCATTGGTTCTGGGACCAGAATACAAACAAGCAGTTCAAGCCAGC ATTAAGGAGTTTGGATTTAACATGGTGGCAAGTGACATGATCTCACTGGACCGCAGCGTCAATGACTTAC GCCAAGAAGAATGCAAGTATTGGCACTATGATGAAAACCTGCTTACTTCAAGCGTTGTCATTGTCTTCCA TAATGAAGGATGGTCAACCCTCATGAGGACAGTCCACAGTGTAATCAAAAGGACACCAAGGAAGTACTTA GCAGAAATCGTGTTAATTGATGACTTCAGCAATAAAGAACACTTAAAAGAGAAACTGGATGAGTATATAA AGCTATGGAATGGCCTCGTGAAGGTATTTCGAAATGAGAGAAGAGAGGGTTTGATTCAAGCTCGGAGCAT TGGCGCACAGAAGGCTAAACTCGGACAGGTTTTGATATACCTTGATGCCCACTGTGAGGTGGCAGTTAAC TGGTATGCTCCACTTGTAGCCCCCATATCTAAGGACAGAACCATTTGCACTGTGCCGATTATAGATGTCA TAAGCGGCAACACATATGAAATTATACCCCAAGGGGGTGGTGATGAAGATGGGTATGCCCGAGGAGCATG GGACTGGAGTATGCTCTGGAAACGGGTGCCTCTGACCTCTCGAGAGAAGAGACTGAGAAAGACAAAAACT GAGCCGTATCGGTCTCCAGCTATGGCGGGTGGATTGTTTGCCATAGAGAAGGACTTCTTCTTCGAACTGG GTCTCTATGATCCTGGTCTCCAGATCTGGGGTGGTGAAAACTTTGAAATTTCATACAAGATCTGGCAGTG CGGTGGCAAATTGTTATTTGTGCCTTGTTCTCGTGTTGGGCACATCTACCGTCTTGAGGGCTGGCAAGGA AACCCCCCACCCCTTTACGTTGGCTCCTCTCCAACTCTGAAGAATTATGTTAGAGTCGTGGAAGTCTGGT GGGATGAATATAAAGACTACTTCTATGCTAGCCGTCCTGAGTCAAAGGCGCTGCCCTACGGGGACATATC TGAGCTGAAGAAATTTCGAGAAGATCACAACTGCAAAAGTTTCAAGTGGTTTATGGAAGAAATCGCTTAT GACATCACTGCCCACTACCCTTTGCCCCCCAGAAATGTCGAGTGGGGTGAAATCCGAGGCCTCGAAACTG CATACTGTATTGATAGCATGGGGAAGACGAATGGAGGCTTCGTGGAGCTAGGACCCTGCCACAGGATGGG TGGGAACCAGCTTTTCCGAATCAATGAAGCAAACCAGCTCATGCAGTACGACCAGTGTTTGACAAAGGGG CCTGATGGATCCAAAGTCATGATCACACACTGTAACCTAAATGAATTTAAGGAATGGCAGTACTTCAAGA GCCTGCACAGGTTTACGCACATCACTTCCGGAAAGTGCTTAGATCGATCGGAGGTCCTGCATCAAGTGTT CATCTCCACCTGTGACTCCAGTAAAATGACTCAGAAGTGGGAGATGAATAACATCCACAGTGTTTGACAA GAACAGAGGAAACCAACAATCTACCTACTGACAAGTACATTTATGGAGGACTGAAAACCGCCTGGAACCT GCTGCAACCATTATTACTAATTTTGTACAGCTCCAAACCTGGAACCTCTCTGATCAGTTGGAAGGGGCAT TGATAAACTGTGATTTTACAATAACATTATCATCTGCAGTGACTGTTTACAAAACTGCTCTTACCTTAAA CTCTAGATGTTTACATCGTTTTTTGTTTTGTTTTATGATGATGTTGGTAATTTGTGCCTTTAACTCGGTT TCCTGAACCGCCGAGTTAAAGCATGTGTTGTCTTCTTTGGGAATACACTCAGGGGTCTGGAAGGCAGTTT GGTTTTTTTTGTTGTTGATGATGTTGTTCTTGTTTTTTAACACACTTGAAAAAAAAAAAAGGTTGGAGTA AGCAGACTTTCACATCCGACTTGGTGATGATCAACCTGCTGTGTATTTAATTTTACATCTTTCGGAAGCA CTGCCACGGGTCGTTGGCCAGGGTGGCCTTCCTTCAGTTACGCTGCTGTTTGAAAGGTGAATTTCAACAC ATTTAGTGCCTCTTTCATTTCTCAGTATATTGTTTGAGAGCTTCTAGTGACACTTCTATGATGGTGACAA TGAATGTCACCTGGGGAACCCGTCTGTTACTCACAGGAGAATTTCTTGGCTATGAAGTGGAATGTTGTAT GGCTGGGTCGAGGACAACAGTGGGCCCAACTCAAGGCTGTTCCGTGGGGCTTGAAGATTCTGTGGCATTA CCTCCCTGGCCTCTGTTCACACCAGCTTCTAGGCTAGCAAAGGAGTCCTTCTTCTAAAAGGAGGGGTTGG TTTTTGCCCATCTACATTTTGCATCTGCTTTCCCCAGTACCAGTCATAGAAAACTAAACATAATTCTCAG TTTGAAACAACACAATAAAGCCCTAGTGTGGACGCCTTAGGTTGGTCCTGAGGTAAAAATCCCCAAGCCC TTGTGAGATGGAAGCCTTAGAGAACCACCTCGAAGCACAAGCTGTGCACAGAGATAAACTGACACTTTGT CTACATTCAAAGGAAATTCCATGAGCATTCTTTTTAATTTAGGAAAATTAGTATCTAATTTTCTATTCTT AGATCTTATTATCTAAGAATACAAATCATCAACAAATGTGGACGACATTCTGCGAAGTTAGATGCTAGCT CTGTAGGGTTCCTTAATTTCTTTTTTAAGAAGTATAAATTTTTTTTATATTCCCCAGGGAAAAGAAGATT TAATTTGAACATTTATTAATACAATACCTACTTTAAGAGAACCAAATGTTGAAAACTTTAATTTCTAGGA AGTCTCTTATTACACCCCCCTTCCCCCAAGATAAAATGTCTACATTGAGTGCCAAAAAAAAAAAAAAAAA A
Notes:	The probe template was PCR amplified from IMAGE:6834581 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6834581 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14750 same embryo
	EMAGE:14747 same embryo
	EMAGE:14745 same embryo
	EMAGE:14749 same embryo
	EMAGE:14746 same embryo
	EurExpress:euxassay_005715 same experiment
	MGI:4824985 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source