EMAGE mouse gene expression spatial database

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EMAGE:14751

Myo1f myosin IF ( MGI:107711)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005689. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005689_01	euxassay_005689_02	euxassay_005689_03	euxassay_005689_04

euxassay_005689_05	euxassay_005689_06	euxassay_005689_07	euxassay_005689_08	euxassay_005689_09

euxassay_005689_10	euxassay_005689_11	euxassay_005689_12	euxassay_005689_13	euxassay_005689_14

euxassay_005689_15	euxassay_005689_16	euxassay_005689_17	euxassay_005689_18	euxassay_005689_19

euxassay_005689_20	euxassay_005689_21	euxassay_005689_22	euxassay_005689_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14751_wholemount_strong.wlz

14751_wholemount_moderate.wlz

14751_wholemount_weak.wlz

14751_wholemount_possible.wlz

14751_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14751_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

embryo

moderate

single cell

moderate expression: see section 08

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7289
Entity Detected:	Myo1f, myosin IF ( MGI:107711)
Sequence:	sense strand is shown >T7289 AAAGAGGGGCTGTTACAAGAGGAACTAGGGCCAAGAGCCTGTGAAGGAGGAGAGCCATGCCAGAGCCACC CCCTAGAACCCTCTGAGCCTGCACCCCTGTCATTACCCACAGGAGCCTACATACCATCCCTCACCATGGG CAGCAAGGAGCGCTTTCACTGGCAGAGTCACAACGTGAAGCAGAGTGGAGTGGATGACATGGTGCTGCTG CCCCAGATCACCGAGGACGCCATTGTGAGCAACCTCCGCAAACGCTTCATGGATGACTACATTTTCACAT ACATCGGCTCAGTGCTAATCTCTGTAAACCCCTTCAAGCAGATGCCCTACTTCACTGACCGAGAAATTGA CCTCTATCAGGGCGCGGCTCAATATGAGAATCCCCCGCACATCTATGCCCTCACCGACAACATGTACCGG AATATGCTGATCGACTGTGAGAACCAGTGTGTCATTATCAGTGGGGAGAGCGGAGCTGGGAAGACAGTGG CTGCTAAATACATCATGGGCTACATCTCCAAGGTGTCTGGTGGGGGAGACAAGGTCCAGCACGTCAAAGA CATAATCCTACAGTCAAACCCACTGCTTGAGGCTTTCGGCAATGCCAAGACCGTGCGCAACAACAATTCC AGCCGCTTTGGCAAGTACTTTGAGATCCAGTTCAGTCGAGGTGGAGAGCCAGATGGAGGCAAGATCTCCA ACTTCCTGCTGGAAAAGTCTCGTGTGGTCATGCAGAATGAGAATGAGAGGAACTTCCACATCTACTACCA GCTTCTGGAAGGGGCCTCCCAGGAGCAGCAGCAGAACCTAGGACTCATGACGCCAGACTACTACTACTAC CTCAACCAATCAGACACCTACAAGGTGGAAGGCACTGATGACCGAAGTGACTTCAGCGAGACCCTGAGCG CCATGCAAGTCATCGGGATTCCGACAAGCGTGCAGCAGCTTGTCTTGCAGCTCGTGGCAGGGATCTTGCA CCTGGGGAACATCAGCTTCTGTGAAGAAGGGAATTATGCCCGCGTGGAGAGCGTAGACCTCTTAGCTTTC CCAGCCTACCTGCTGGGCATAGACAGTGGGCGGCTGCAAGAGAAGCTGACCAGCCGTAAGATGGACAGCA AATGGGGTGGACGCAGTGAGTCCATTGATGTGACCCTCAATGTGGAACAGGCAGCCTACACTCGTGACGC ACTGGCCAAGGGACTCTATGCCCGCCTCTTCGACTTCCTGGTGGAGGCCATCAACCGTGCCATGCAGAAG CCTCAGGAAGAATACAGCATCGGCGTGCTTGACATCTACGGCTTTGAGATCTTCCAGAAAAATGGCTTTG AGCAGTTCTGCATCAACTTCGTCAATGAAAAGCTGCAGCAGATTTTTATTGAGCTCACGCTGAAGGCAGA GCAGGAGGAGTATGTGCAAGAGGGCATCCGCTGGACCCCCATCGAATACTTCAACAACAAAATTGTGTGT GACCTCATCGAAAACAAGCTGAGCCCCCCAGGCATCATGAGCGTGCTAGATGATGTGTGTGCCACGATGC ATGCTACCGGTGGCGGCGCGGACCAGACACTGCTACAAAAGCTGCAAGCGGCAGTGGGCACCCACGAGCA CTTCAACAGCTGGAGCGCGGGTTTCGTCATCCATCACTATGCAGGCAAGGTCTCCTATGATGTCAGTGGC TTCTGTGAGAGGAATCGAGATGTGCTGTTCTCTGATCTTATAGAACTGATGCAGTCCAGTGACCAGGACT TCCTACGGATGCTCTTTCCTGAGAAGCTGAATATAGACAAGAAGGGCCGCCCCAGTACTGCTGGCTCCAA GATCAAGAAACAAGCCAACGACCTGGTGTCCACGCTGAAGAAGTGCACACCCCACTACATCCGCTGCATC AAACCTAACGAGACCAAGCGCCCCCGGGACTGGGAGGAAAGCAGAGTGAAGCACCAGGTGGAGTACCTGG GCCTGCGGGAGAACATCCGAGTGCGAAGGGCAGGCTTCGCCTACCGTCGGCAGTTCTCCAAATTCCTGCA AAGGTATGCCATCCTGACCCCTGAGACATGGCCACGGTGGCGTGGAGATGAACGCCAGGGTGTCCAGCAC CTGCTTCGTGCTGTCAACATGGAGCCAGACCAGTACCAGATGGGGAGTACCAAGGTCTTTGTTAAGAATC CTGAGTCGCTTTTCCTCCTGGAAGAGATGCGAGAGAGGAAGTTTGACGGCTTCGCTCGCACTATCCAGAA AGCCTGGCGGCGCCACGTAGCAGTCCGAAAGTATGAAGAGATGCGGGAAGAAGCTTCTAACATCCTACTG AACAAGAAGGAACGAAGGCGAAACAGCATCAACCGGAACTTCGTTGGGGACTATCTGGGACTGGAGGAGC GGCCTGAGCTGCGCCAGTTCCTGGCTAAAAGGGAACGAGTGGACTTTGCCGACTCAGTCACCAAGTATGA CCGCCGCTTCAAGCCTATCAAACGGGACTTGATCCTGACCCCAAAACGTGTGTATGTGATTGGGAGAGAG AAGGTGAAAAGGGGACCAGAGAAGGGTCTAGTGCGTGAAGTCCTGAAGAAGAAACTGGATATCCAGGCCC TCCGAGGGGTGTCTCTCAGCACCCGACAGGATGACTTCTTCATCCTTCAAGAAGAGGCTGCGGACAGCTT CTTAGAAAGTATCTTCAAGACCGAGTTTGTCAGCCTTCTGTGCAAGCGTTTTGAAGAGGCAGCGCGCAGG CCCCTTCCCCTCACCTTCAGTGACCTACTACAGTTCCGGGTGAAGAAAGAAGGCTGGGGCGGAGGTAGTA CCCGAAATGTCACCTTCTCTCGCGGGACAGGTGACTTGGCTGTGCTCAAGGCTGGGGGTCGAGCTCTCAC TATCAGCATTGGGGACGGATTGCCTAAGAGCACCAAGCCTACACGGAAGGGGTTAGCCCAGGGTAGACCT CGAAGGTCAGCGCAGGCTCCCACTCGAGCAGCCCCTGGGCCTCCCAGAGGTTTGAATCGAAATGGGGTAC CACCCTCTTCCCAGGTAAGGTCCCTACCCCTGGAGATCACATCTGGTAGGAGTTCCCAGAGGCCTCCACG GGGCCCTCCATCCTCAACCCTAGGAGCCAGCAGACGTCCTCGAGCACGGCCACCTTCAGAGCACAGTACA GAATTCCTTAATGTGCCTGACCAGGGTGTGGCAGGCATGCAAAGGAAGCGAAGTATAGGGCAGAGACCTG TGCCAGGAGTGGGCCGGCCTAAGCCCCAACCACGGACACATGGCCCGAGGTGCCGGGCACTGTACCAATA CATAGGCCAGGATGTGGATGAGCTGAGCTTTAACGTGAACGAAGTCATTGAGATCCTCATAGAAGACTCA TCTGGCTGGTGGAAGGGTCGGCTTCATGGCCAGGAAGGTCTCTTTCCAGGAAACTATGTGGAGAAGATCT GATCGTCCATCCACCAGCCTGCCTGCTTGCCTGCCTGGGATCCAAACCCTGCCAGTGGAAGCCTTGGTTA TCTTATCTGCACTGGCCAGAATACTCAGGCTGTGGCCTCAAGCCCAGTGGGACCCCTGTGTATGGAGGAT GAGCACTGTAGCACCCCATGCCTGGTACTGCCTTCCTGTGGGTTGACTTGCTATCTAAGTCTCCATTTTC TCCTGTGTCCAATAGTAGTTGCTGCAAAACCTACCTCCCAGCTGCCTTGTGTGTATGTAACAAATCATGT CCTGCCCTGGGAGCAAAGTGCTGTGCACTGCCCAGAGTTAGGAGACGTGGACAGTTAGACCTTTCCACTT TCTCTCATCACTGAGGTAATAAACATGTGTCAAGTAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4167558 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4167558 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14753 same embryo
	EMAGE:14754 same embryo
	EMAGE:14755 same embryo
	EMAGE:14752 same embryo
	EurExpress:euxassay_005689 same experiment
	MGI:4826562 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source