EMAGE mouse gene expression spatial database

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EMAGE:14756

Eif3b eukaryotic translation initiation factor 3, subunit B ( MGI:106478)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005685. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005685_01	euxassay_005685_02	euxassay_005685_03	euxassay_005685_04

euxassay_005685_05	euxassay_005685_06	euxassay_005685_07	euxassay_005685_08	euxassay_005685_09

euxassay_005685_10	euxassay_005685_11	euxassay_005685_12	euxassay_005685_13	euxassay_005685_14

euxassay_005685_15	euxassay_005685_16	euxassay_005685_17	euxassay_005685_18	euxassay_005685_19

euxassay_005685_20	euxassay_005685_21	euxassay_005685_22	euxassay_005685_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14756_wholemount_strong.wlz

14756_wholemount_moderate.wlz

14756_wholemount_weak.wlz

14756_wholemount_possible.wlz

14756_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14756_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7160
Entity Detected:	Eif3b, eukaryotic translation initiation factor 3, subunit B ( MGI:106478)
Sequence:	sense strand is shown >T7160 GCGGCCGAGCCGGGTGAGAAGCAGCGCGGGCCCATGCAGGACGCTGAGAACGTGGCGGTGCCCGAGGCGG CCGAGGAGCGGGCTGAGCCCGCGAGGCAGCAGCCGGCCTCCGAGTCGCCGCCGACGGACGAGGCGGCAGG GTCTGGGGGGTCCGAAGTGGGGCAGACGGAAGACGCCGAAGAAGACGCCGAGGCCGGACCGGAGCCCGAA GTGAGGGCCAAGCCGGCGGCCCAATCCGAAGAGGAAACGGCGACGAGTCCGGCCGCCTCGCCCACGCCGC AGTCGGCGGAGCGGTCCCCGTCGCAGGAGCCCTCTGCTCCCGGGAAGGCAGAGGCCGTTGGCGAGCAGGC CCGGGGGCACCCCTCGGCTGGAGCGGAGGAGGAGGGCGGGTCTGATGGGAGCGCGGCTGAGGCGGAGCCG CGGGCTCTGGAGAACGGAGAGGCAGATGAGCCCTCCTTCAGCGACCCCGAGGATTTCGTAGACGACGTGA GCGAGGAAGAACTACTTGGTGATGTACTCAAAGATCGGCCTCAGGAAGCGGACGGGATTGACTCGGTGAT TGTTGTAGACAACGTCCCTCAGGTGGGGCCAGACCGACTTGAGAAACTCAAAAATGTCATCCACAAGATC TTCTCCAAGTTTGGGAAAATCATAAATGACTATTACCCTGAGGAGGACGGAAAGACGAAAGGGTACATCT TTCTGGAGTACGCTTCTCCCGCCCATGCTGTGGATGCTGTGAAAAACGCCGATGGCTACAAGCTGGACAA GCAGCACACGTTCCGGGTCAACCTCTTCACCGACTTTGACAAGTACATGACGATCAGCGATGAGTGGGAC ATTCCAGAGAAGCAGCCTTTCAAAGACCTGGGCAATTTGCGTTACTGGCTGGAAGAGGCAGAGTGTAGAG ATCAGTACAGTGTGATTTTTGAGAGCGGAGACCGGACGTCCATCTTCTGGAATGACGTCAAGGACCCAGT CTCAATTGAAGAAAGAGCGAGATGGACAGAGACGTACGTGCGCTGGTCCCCTAAGGGTACCTACCTGGCC ACTTTCCATCAGCGGGGTATCGCCCTCTGGGGAGGGGACAAGTTCAAGCAGATTCAGAGGTTCAGCCACC AAGGCGTGCAGCTCATCGACTTCTCTCCGTGTGAAAGATACTTGGTAACGTTCAGCCCTCTGATGGACAC TCAGGATGACCCTCAGGCCATCATCATCTGGGACATCCTCACGGGCCATAAGAAGAGAGGTTTTCACTGC GAAAGCTCAGCCCACTGGCCCATCTTCAAGTGGAGCCATGATGGCAAATTCTTTGCAAGAATGACCCTGG ATACTCTTAGCATCTATGAAACACCTTCCATGGGCCTTTTGGACAAGAAGAGTTTGAAGATCTCTGGAAT TAAAGACTTTTCTTGGTCTCCGGGTGGTAACATCATTGCTTTTTGGGTGCCTGAAGACAAAGATATTCCA GCCAGGGTGACCCTGATGCAGCTCCCCACCAGACAGGAGATCCGAGTGAGGAATTTGTTCAATGTGGTTG ATTGCAAGCTGCACTGGCAGAAAAATGGAGATTACCTATGTGTAAAAGTGGACAGGACTCCGAAAGGCAC ACAGGGAGTTGTCACAAATTTTGAAATATTCCGAATGAGGGAGAAGCAGGTACCTGTTGATGTTGTTGAG ATGAAAGAAACCATCATAGCCTTTGCCTGGGAACCCAATGGCAGTAAGTTTGCCGTGCTTCATGGTGAGG CCCCTCGGATCTCTGTCTCCTTCTACCACGTGAAGAGCAACGGGAAGATTGAACTCATCAAGATGTTTGA CAAGCAGCAAGCCAACACCATCTTCTGGAGTCCCCAGGGGCAGTTCGTGGTGCTGGCCGGGCTGAGGAGC ATGAACGGTGCCTTGGCGTTTGTCGACACTTCAGACTGCACCGTCATGAACATTGCAGAGCACTACATGG CCTCCGATGTTGAATGGGACCCAACTGGCCGCTACGTGGTTACCTCTGTGTCGTGGTGGAGCCACAAGGT GGACAATGCTTACTGGCTGTGGACCTTCCAAGGTCGCCTTCTGCAGAAGAACAACAAGGACCGCTTCTGC CAGCTGCTCTGGAGACCAAGGCCCCCAACACTCCTCAGCCAGGATCAGATAAAGCAAATTAAAAAGGATC TGAAGAAGTACTCCAAGATCTTTGAGCAGAAGGATCGCTTGAGCCAGTCCAAAGCTTCAAAGGAACTGGT GGAAAGAAGGCGGACCATGATGGAGGACTTCCGGCAATACCGGAAAATGGCCCAGGAACTCTACATGAAG CAGAAGAACGAGCGTCTAGAGCTACGAGGAGGGGTGGACACTGACGAGCTGGACAGCAACGTGGATGACT GGGAGGAAGAGACCATTGAGTTTTTTGTCACCGAGGAAGTCATTCCTCTGGGAAGTCAGGAGTGATCTCA GCACTGTGTGTCACCGTGCCGTGCCCAGGATCCGAGGCCACCCTTCACGGCGAGCCTCGTGCTGGGCTCT TACCTGTGCTTTCTTGCTCTGGACTGCGAGTGCACCCAGGGTTCTTCTATCCCGACACAGCTCTGTGCCT TCAGCCCCAGGTCCACAGCGGGGACACTCGAGCCTCTGCTCTCACCTTGCCCTGTTTGGGTTTTTATCAG CCGTGTCTGCATTGGAGCCTCACTGCAGTGTTCCTGGTGGCTCCTCTTAACTCCTGCTGCTGTCCATGGG GTTTCCAGAGCCTTCACCTACAGCTCAGTGGGGTTTCCATTCACGGACTTGCTGTTCCTGTGGGCCAGTG TCGTGTGGCCTGGAGACCATCAGGAGTCTGCACACCCACCACTTACTTGTGCCAGAAACTCTGGAGGCAC CCACACCTCCCACCTGTTCAGGTTGTGGCAGCCTCTGTGCAGGTTGGATGTGGAAATAAAAGCAACCCTG TGTGAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4023277 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4023277 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14757 same embryo
	EMAGE:14758 same embryo
	EurExpress:euxassay_005685 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source