EMAGE mouse gene expression spatial database

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EMAGE:14758

Habp2 hyaluronic acid binding protein 2 ( MGI:1196378)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005686. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005686_01	euxassay_005686_02	euxassay_005686_03	euxassay_005686_04

euxassay_005686_05	euxassay_005686_06	euxassay_005686_07	euxassay_005686_08	euxassay_005686_09

euxassay_005686_10	euxassay_005686_11	euxassay_005686_12	euxassay_005686_13	euxassay_005686_14

euxassay_005686_15	euxassay_005686_16	euxassay_005686_17	euxassay_005686_18	euxassay_005686_19

euxassay_005686_20	euxassay_005686_21	euxassay_005686_22	euxassay_005686_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14758_wholemount_strong.wlz

14758_wholemount_moderate.wlz

14758_wholemount_weak.wlz

14758_wholemount_possible.wlz

14758_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14758_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

pancreas

moderate

regional

moderate expression: see section 09 10 11 12 14 15 16

stomach pyloric region

moderate

regional

moderate expression: see section 04 07 08 09 weak expression: see section 05 06

midgut

moderate

regional

moderate expression: see section 11 12 13 15 16 17 weak expression: see section 14

liver

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7589
Entity Detected:	Habp2, hyaluronic acid binding protein 2 ( MGI:1196378)
Sequence:	sense strand is shown >T7589 AGAGAAACTGCAGAGGGAGAGAACAGAGAGGCCTTCAACTACACAGATATTTGTCAGGATGTTGGTGTTC CGTGTCCTGCTGCTAATCGCCCTGGTGGGGAAGTCAGTCATTGGGCTCTCACTGATGTCCTTCATTGCGC CCCCAGACCCAGATCCATGCCAGTCCAACCCCTGTGAACACGGCGGGGACTGTATCATCAGAGGGGATAC CTTCAGTTGCAGCTGCCCAGCCCCCTTCTCGGGGAGCCGGTGCCAGACTGCACAAAACAAGTGCAAGGAC AACCCATGTGTCCATGGTGATTGCCTCATTACCCAGAAGCACCCCTACTACCGCTGTGCCTGCAAATACC CTTACACGGGACCAGACTGCTCCAAAGTGCTTCCGGCATGCAGGCCAAACCCCTGCCAGAATGGCGGAGT CTGTTCCCGACACAGACGGAGATCCAGGTTTACCTGTGCCTGTCCAGACCAGTATAAGGGGAAATTCTGT GAAATAGGTCCGGACGACTGTTATGTCGGTGATGGCTACTCTTACCGAGGCAAAGTGAGTAAGACAGTCA ACCAGAACCCATGCCTTTACTGGAACTCCCACCTCCTCTTGCAGGAGACTTATAACATGTTTATGGAGGA TGCAGAGACCCACGGGATCGCAGAGCACAACTTCTGCAGAAACCCAGATGGAGACCACAAACCCTGGTGT TTCGTCAAGGTGAACAGTGAGAAGGTGAAATGGGAATACTGTGATGTCACAGTCTGTCCAGTGCCTGACA CCCCTAACCCAGTGGAAAGCCTTCTGGAGCCTGTGATGGAGCTGCCAGGGTTCGAGTCCTGCGGGAAGAC GGAGGTAGCTGAACACGCAGTCAAGCGTATCTACGGGGGCTTTAAGAGCACAGCAGGCAAGCACCCGTGG CAGGTGTCCCTGCAGACCTCACTGCCGTTGACCACCTCCATGCCCCAAGGCCACTTCTGTGGGGGCGCCC TGATCCACCCCTGCTGGGTGCTCACTGCAGCCCACTGTACCGACATAAACACCAAGCATCTAAAAGTTGT ACTAGGGGATCAGGACCTGAAGAAGACAGAATCCCATGAACAGACCTTCAGGGTGGAAAAAATACTGAAG TACAGTCAGTATAATGAAAGAGATGAGATTCCCCACAATGACATTGCTTTGCTCAAGTTAAAGCCAGTGG GTGGTCACTGTGCTCTGGAATCCAGATATGTGAAGACTGTATGTTTGCCCAGCGACCCCTTTCCCTCTGG AACTGAGTGCCACATCTCTGGCTGGGGTGTTACAGAAACAGGGGAAGGGTCCCGCCAGCTCCTGGATGCT AAAGTCAAGCTAATCGCTAACCCTTTGTGCAACTCCCGACAACTCTATGACCACACCATCGATGACAGTA TGATTTGTGCGGGGAACCTTCAGAAGCCCGGATCAGACACCTGCCAGGGTGACTCGGGGGGCCCTCTAAC CTGTGAGAAGGATGGAACTTACTACGTCTACGGGATTGTAAGCTGGGGCCAGGAATGTGGGAAGAAGCCA GGAGTCTACACTCAAGTCACCAAGTTCCTGAATTGGATAAAGACCACCATGCACAGGGAGGCTGGCCTCT GAGGTGCTGTCTGCAGAGCCTTGAAGCCCCTCGTCTTTAGCACCAAACAAGAGGGGCTTCCTGCGTAGCC AGGGATGTCCTGGGTCCATGCTCTAGACAGAGGCATTGTTGCCCAGCCTGGGCCTTTCCAGACTGGCACT ATATCATTTCATGAGGCCTTTTCTCTCTGGGAACCAACAAGAAATACATTATCTTTGCCCCCGTTCTGAC AAGTGTACCTTCTAGATAACCAGAATTCAGAGACGTTCTCCACCATGGAGACTGCGAATCTGAGATCCCT GCTTGCCCCGCACCAGGAGATGTTTCCTTGACTCTTTATCTTTTGTCCATACCATCAGGGTCCTCCACAG AACCAGCCATTCTCATGCAGCCATCAGAGAGAAGCCCCCAAAACCACAAGCTCTTTGTTTGCTTTCAGAG TTGTTATTTTAATAAAGGCAGATGTGGGCACGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4242577 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4242577 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14757 same embryo
	EMAGE:14756 same embryo
	EurExpress:euxassay_005686 same experiment
	MGI:4825314 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source