EMAGE mouse gene expression spatial database

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EMAGE:14767

Tspyl1 testis-specific protein, Y-encoded-like 1 ( MGI:1298395)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005721. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005721_01	euxassay_005721_02	euxassay_005721_03	euxassay_005721_04

euxassay_005721_05	euxassay_005721_06	euxassay_005721_07	euxassay_005721_08	euxassay_005721_09

euxassay_005721_10	euxassay_005721_11	euxassay_005721_12	euxassay_005721_13	euxassay_005721_14

euxassay_005721_15	euxassay_005721_16	euxassay_005721_17	euxassay_005721_18	euxassay_005721_19

euxassay_005721_20	euxassay_005721_21	euxassay_005721_22	euxassay_005721_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14767_wholemount_strong.wlz

14767_wholemount_moderate.wlz

14767_wholemount_weak.wlz

14767_wholemount_possible.wlz

14767_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14767_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T7436
Entity Detected:	Tspyl1, testis-specific protein, Y-encoded-like 1 ( MGI:1298395)
Sequence:	sense strand is shown >T7436 GTTGTTTGTAGGCGGAGGTCTGCGCGACTAGACCCGACATGAGCAGCCCGGAGCGGGACGAGGGGACCCC TGTCCCCGACAGCCGCGGCCACTGCGATGCTGACACTGTCTCGGGGACCCCAGACCGGCGACCGCTGCTC GGCGAGGAGAAGGCGGTGACAGGTGAGGGCCGCGCAGGGATCGTGGGGAGCCCCGCGCCCCGAGATGTCG AGGGCCTTGTCCCCCAGATTCGGGTGGCCGCAGCTAGGCAGGGAGAGTCACCTCCTTCCGTTCGGGGCCC CGCGGCAGCGGTGTTCGTGACACCGAAATATGTGGAGAAGGCCCAAGAAACTCGGGGAGCGGAGTCCCAG GCCCGTGACGTGAAGACGGAACCGGGCACCGTCGCGGCTGCAGCGGAAAAGTCAGAAGTGGCCACGCCAG GGAGCGAGGAGGTGATGGAGGTGGAGCAGAAGCCCGCGGGCGAAGAAATGGAGATGCTGGAGGCGAGTGG AGGAGTGAGGGAGGCGCCGGAGGAGGCAGGGCCCTGGCACCTGGGCATAGATCTTCGCAGGAACCCGCTG GAGGCCATCCAGCTGGAACTGGACACTGTGAATGCTCAGGCCGACAGGGCCTTCCAACATCTGGAGCAGA AGTTTGGGCGAATGCGACGACACTACCTGGAACGGAGGAACTACATCATTCAGAATATCCCAGGCTTCTG GATGACTGCCTTCCGGAACCACCCTCAGTTGTCCGCCATGATTAGGGGTCGGGATGCAGAGATGTTAAGG TACGTAACCAGTCTGGAGGTGAAGGAACTGAGACACCCTAAGACTGGCTGCAAATTCAAGTTCTTTTTTC GAAGAAACCCCTACTTCAGAAACAAGCTGATTGTCAAGGAATATGAAGTGAGATCATCTGGTCGAGTGGT GTCTCTTTCCACTCCAATCATTTGGCGGAGAGGGCATGAGCCCCAGTCCTTCATTCGCAGAAACCAAGAT CTCATTTGCAGCTTCTTCACCTGGTTCTCGGACCACAGCCTTCCAGAATCTGACAGAATCGCTGAGATTA TCAAAGAGGATCTGTGGCCAAACCCACTGCAATACTACCTGTGTCGTGAAGGAATCCGTAGACCCCGACG TCGCCCAATAAGGGAGCCAGTGGAGATTCCCAGGCCTTTTGGCTTCCAGTCAGGTTAATCTTTGCATTTG AAAATGCGGCCGGAACTGGTCCCTTCTCACCTGTGCTTGAGCAAATGCAACGGGTATGCAGTGTTTTGAT TTCTTTCCTCCTGACTTTTCTGCATCTTCTATCTGGATAGTCTCCACCTCGAGATTAAGACTGGACATGC TTCTGCTTTCCCATGGCCTTCATGCTGTTCTGAATTAGTATCTGCTGGTTCACGGCCCTGGCTTATACCC TTTCTAAGCTCAACAATGAAACTGTATGTTACACTGCCTTTACACTGTTTGGACCCTATTGTTTTCAGTC TTACCTGTTTACTGCCACCTAGATGTCTTGCTTTAACTGGGGGCCCTTCTATTTATCTGTTCATCATGTG GCAACGTGGACACAGAGTTTTTGGACAGTTGCTGAGCCTAAACATTGAAGGAGTGGGCCACTGTCTGTGT GATTTTTTTTTTTTTTTTTTTGGCACCACTTTCTAGGTTATATCACACTCCATTGTGTTTGCCAGTGCTC CTCACCCTCACTCTCCCACCTTTCTCCTGCTATTTTAAAGAAAAGTAGTTCAGAAACATCTGTAAGGCCA GGAGTCATTTATACTTAGCATTTCCTGAGAAGATACTCTGTAAAAGAGGAAACAACTCTGCCTTGTCTCC TCTCTTACCCACTCCCCAGCCTGGTTAGCAAGCTCTAAAAAGCTTCCAGGGAGACAGCTAGATGGAAACA GCTGAGCCCTTCCTAATATATGGAAGAACTAAAGAAGTTTTGATGGGTACTCTAAGCAGAAAAAGTGAGC TGAAAAGGCTTAGAATCAAAATAAGTCAGATAAAAATTGGGCTGAGAATGAAGACAGGACACAGCTTACA CATCTTGGTCAGTCAGTGGTGATGGGAAGAGAACTAATTTCTCAGGAAAAAATATTTTCTCTGAGTGTTG AATGTTGCCTAGATAGTGCTTCTTTAAAGAAGCAGTGCAAGTTTGAGTTATTTTCCTCTCTTTATATGTG ATCTGATATAAGTGAAAGTATAATATTTTGCCCATCTTTTACCAATATTTAGTAAAATGCTACTTGTTTC AACTTACTGAAAAAATTTAAACACTTTTGTTTGCATTTATTTGAAACCATTTGGAATATTGTAAGACAAC TCACTCTGTAGACCAGGCTGGTCTTGAACTCAGAAATCCGCCTGCCTTTGCCTCCCAAGTGCTGGGATCA AAGGCGTGCGCCACCACTGCCTGGCTAACAGTAGTCTTTATAATACTGAGACTGAATATTCGTAACAGTT TTCGATATTACTCCAAATTCTTAGAAACGGCTTGGATAATTGGTCTTAATCAAATGTGTCAAATTATTGT TTTCATGGAAAAGTAAATATGTGACTTGATTGTCTTGATATAGTTATAAAAGTACTTTGTCAGCACTGTA TATTTGTTGCCATTTTTATGCATGATGGTAAATAAAAGCATGGCATCCTGTTAAAAAAAAAAAAAAAAAA AAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:4196841 using vector (pCMV-SPORT6) specific primers. Forward Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG; Reverse Primer - name:RZPD sp6, sequence:ATTTAGGTGACACTATAG. Anti-sense probe was then transcribed from the PCR amplified template using Sp6 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:4196841 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14769 same embryo
	EMAGE:14768 same embryo
	EMAGE:14770 same embryo
	EMAGE:14766 same embryo
	EMAGE:14765 same embryo
	EurExpress:euxassay_005721 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source