EMAGE mouse gene expression spatial database

EMAGE:14768

Mrp63 mitochondrial ribosomal protein 63 ( MGI:1915090)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005717. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005717_01	euxassay_005717_02	euxassay_005717_03	euxassay_005717_04

euxassay_005717_05	euxassay_005717_06	euxassay_005717_07	euxassay_005717_08	euxassay_005717_09

euxassay_005717_10	euxassay_005717_11	euxassay_005717_12	euxassay_005717_13	euxassay_005717_14

euxassay_005717_15	euxassay_005717_16	euxassay_005717_17	euxassay_005717_18	euxassay_005717_19

euxassay_005717_20	euxassay_005717_21	euxassay_005717_22

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Expression Pattern Description

Spatial Annotation:

Annotation colour key:

wholemount mapping

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Morphological match to the template:

Annotation Validation:

spatial mapping by EMAGE editor

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8835
Entity Detected:	Mrp63, mitochondrial ribosomal protein 63 ( MGI:1915090)
Sequence:	sense strand is shown >T8835 TGTCCCGGGCCGGTTGGTACGCGAGGAGGCACCATGTTCCTCACCGCTGTCCTCCTCCGCGGCCGCATTC CGGGCAGGCAGTGGATCGGGAAGCACCGGCGGCCGCGTACCGTGTCTTTCCAAGCGAAGGAGAGCATGAT CCGTCGCCTGGAGGTGGAGGCCGAGAACCACTACTGGCTCAGCATGCCCTACATGACAGCAGAGCAGGAG TGCGGCCACGCCGCGGAGCGACGGGCCCAGGCTTTTGAAGCCATCAAGGCAGCGGCCACTTCCAAGTTCC CTAAGCATAGATACATTGCAGACCAGCTAGACCATCTCAACATCTCGAAGAAATGGTCCTAACCCTTCAA GATTATGGATACCGGACTGCTCTATCCTTACTTGGTCCTGGAGCTGAGAAAATGTAATTTATGTACCAGT GTGACCCCTTCGACCAGTATCTTATTAAAGCAAACATGATTGATTAATAAAAAAAAAAAAAAAAAAAAAA AAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6411308 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6411308 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14769 same embryo
	EMAGE:14770 same embryo
	EMAGE:14767 same embryo
	EMAGE:14766 same embryo
	EMAGE:14765 same embryo
	EurExpress:euxassay_005717 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source