EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:14770

Rasgrp1 RAS guanyl releasing protein 1 ( MGI:1314635)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005719. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005719_01	euxassay_005719_02	euxassay_005719_03	euxassay_005719_04

euxassay_005719_05	euxassay_005719_06	euxassay_005719_07	euxassay_005719_08	euxassay_005719_09

euxassay_005719_10	euxassay_005719_11	euxassay_005719_12	euxassay_005719_13	euxassay_005719_14

euxassay_005719_15	euxassay_005719_16	euxassay_005719_17	euxassay_005719_18	euxassay_005719_19

euxassay_005719_20	euxassay_005719_21	euxassay_005719_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14770_wholemount_strong.wlz

14770_wholemount_moderate.wlz

14770_wholemount_weak.wlz

14770_wholemount_possible.wlz

14770_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14770_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

telencephalon mantle layer

moderate

regional

moderate expression: see section 10 11 12 13 weak expression: see section 14

olfactory lobe

moderate

regional

moderate expression: see section 08 weak expression: see section 09 10 14 15 16

nasal cavity olfactory epithelium

moderate

single cell

moderate expression: see section 11 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T9208
Entity Detected:	Rasgrp1, RAS guanyl releasing protein 1 ( MGI:1314635)
Sequence:	sense strand is shown >T9208 CGCACAAACTTTGTGCCTGCGGGTGAGCCTGGGTCGGCTCGCCGGGCTCCAAGAGTGGTCGGCTCCGGGC TGCGAGGGCCAGCGGCGGCCGAGGGATGCGCGGCGCCCGGCCGAGCCGATCCCACCGCTCTAGGTGAGAC GGCTCCAGGGGCGCAGAGAGCCGCGACCATGGGAACCCTGGGCAAGGCGAGAGAGGCTCCGCGGAAACCT TGCCATGGCTCCAGAGCTGGCCCCAAAGCAAGACTAGAGGCCAAATCAACCAACAGCCCTCTCCCTGCCC AGCCCAGCTTGGCCCAGATCACCCAGTTCCGAATGATGGTGTCCCTGGGACATTTGGCCAAAGGAGCCAG CCTGGATGATCTTATTGACAGCTGCATTCAATCTTTTGATGCGGATGGAAACCTGTGTCGGAATAACCAA CTGTTACAAGTCATGCTAACCATGCACCGGATCATCATCTCCTCAGCCGAGCTGCTACAAAAAGTCATGA ATCTATATAAGGATGCCCTGGAAAAGAATTCTCCAGGAGTTTGCCTGAAGATCTGCTATTTTGTCAGGTA TTGGATAACAGAATTCTGGATCATGTTCAAGATGGATGCCAGCTTGACCAGCACCATGGAAGAGTTCCAA GACCTGGTGAAAGCCAATGGTGAGGAGACCCACTGCCACCTCATCGACACGACCCAAATTAATTCTCGAG ACTGGTCCAGGAAACTGACTCAGAGGATAAAATCGAATACTAGCAAGAAGCGCAAAGTGTCCCTGCTGTT TGACCATCTGGAACCTGAAGAACTGTCTGAACACCTCACCTACCTTGAGTTCAAGTCCTTCCGACGGATA TCTTTCTCTGATTACCAAAATTACCTTGTAAATAGCTGCGTAAAAGAGAACCCCACCATGGAGAGGTCCA TTGCCCTGTGCAATGGCATCTCCCAGTGGGTACAACTGATGGTTCTCAGCCGTCCCACCCCGCAGCTCCG GGCAGAGGTCTTCATCAAGTTCATCCATGTGGCTCAGAAACTCCACCAGCTACAGAACTTCAACACGCTG ATGGCTGTGATCGGGGGACTGTGTCACAGCTCCATCTCCAGGCTTAAGGAAACAAGTTCACATGTCCCAC ATGAGATCAATAAGGTTCTGGGCGAGATGACTGAACTGCTGTCCTCCTGCAGAAACTATGACAACTACAG GCGAGCCTATGGGGAGTGCACCCACTTCAAAATCCCCATACTGGGTGTGCACCTCAAGGACCTCATATCC CTGTATGAGGCTATGCCCGACTATCTGGAAGATGGGAAGGTGAATGTCCAAAAGCTCCTGGCCCTTTACA ATCATATCAATGAATTGGTCCAGCTGCAGGAGATGGCCCCACCATTGGATGCCAACAAGGACTTGGTGCA TCTGCTGACGTTATCCCTGGATCTATACTATACAGAAGATGAAATCTATGAGCTTTCCTATGCCCGGGAG CCGAGGAACCACAGAGCTCCGCCACTGACACCTTCGAAGCCACCAGTTGTAGTAGACTGGGCCTCTGGAG TGTCTCCCAAACCTGACCCAAAGACCATCAGCAAACACGTCCAAAGGATGGTGGATTCTGTCTTTAAGAA CTATGATCTCGACCAGGATGGCTATATCTCTCAGGAGGAGTTTGAGAAGATCGCTGCAAGCTTTCCATTT TCCTTCTGTGTGATGGACAAAGATAGGGAAGGCCTCATCAGCAGAGACGAGATCACGGCCTACTTCATGA GGGCCAGCTCCATCTATTCCAAGCTGGGCCTGGGCTTTCCACACAACTTTCAAGAAACCACTTACCTGAA GCCCACCTTCTGTGACAACTGTGCTGGCTTTCTCTGGGGCGTGATCAAGCAAGGCTATCGCTGTAAAGAC TGCGGGATGAACTGCCACAAACAGTGCAAAGACCTGGTGGTGTTCGAGTGCAAGAAGCGAATCAAGAGCC CAGCAATATCCACAGAGAACATCAGCTCTGTGGTACCAATGTCCACCCTTTGTCCACTGGGAACCAAAGA TCTGCTCCATGCACCCGAAGAAGGATCTTTCATTTTCCAAAACGGAGAGATTGTGGACCACAGTGAGGAG AGCAAGGATAGGACCATCATGCTCCTCGGAGTGTCCTCCCAGAAAATTTCAGTTCGGCTGAAGAGGACTG TTGCCCACAAGAGCACCCAAACAGAATCGTTCCCGTGGGTTGGTGGCGAGACGACCCCTGGTCACTTTGT GCTGTCTTCTCCAAGGAAGTCGGCGCAGGGCGCTCTTTATGTGCACAGTCCAGCATCTCCATGCCCCAGC CCAGCACTGGTCCGAAAGCGGGCATTCGTCAAGTGGGAGAACAAAGAGTCCCTTATAAAACCAAAACCAG AACTTCACCTCCGGCTCCGGACCTACCAAGAACTGGAACAGGAAATAAATACCCTGAAAGCAGATAACGA TGCTCTGAAGATCCAACTGAAGTACGCACAGAAGAAAATAGAATCCCTGCAGCTTGGGAAAAGCAATCAT GTCTTAGCCCAGATGGACCACGGTGATAGTGCTTAATCCAGAATTTCAAGGACCAGAATCTGCAGGAGGG TATACTGGGATCTCACTTCAAAACTCATTGCAGACGTTCAGCACCTTTAGATTGACTGACTTTAAAAGGG TAGAGCTGGCCACTACTGTTGACATCCTTGGTTTTTACCTAAACGGTTTTTAATGTGAGTCGTTGGTTTT CAGTCAGTTGAATCAAAGGGAAGAAAAGCTCAAGCATGCAAAGTCTGAGCATTCCTATTCTAACCTTCTT TCTTTACTGAAACCAATACCAAACATGCGCTAAAATAAGTCTAGAGTTTCCTGAGGGTGTCCCAGGCAGC CCGCTTTACTGATTCATACTTAGATAAAGAGTCCCTGGTGAATCCATTTGCTGCCTTCCGCAAACTGACT TACTTTGTTCTACCCAAACCCCGCTCATAACGGAAACAATAGCCTAAGCGTGGTGACTTTGACATGCCTG CATTGTTTGTGAAACTGATCCCTACAGTGTTCGCTACCTCAGACCTCATCAAGAGCCTCTTTCCCCACTG TCCTTCACTTGCCCGCCTCCTTCCTCCCTTGCTTATAGGCACATCAGCACATCTACCTAGAAGTGAACTC CTAGCGATGTAGCCTGGTTTTAAATCCAGAGCTTCTTAATTGAACTTGACATTGTCTAATTGCAGGCCAA CTTATAGCTCTAGGTCTTAGTCATTATGAAATACAAAATAAAACTCACTCACTACCCACAAACAGCAATT ACCATGGCATACACACAGCTAAATAGCTAAGTCAAACCACAATAACACGTTTATCCAGATAGTCTTTCTA TAGTGGGTAGAATTAAGAATCTGTGACTTTTTAAAAGAAATGGTCTGTGAACAGTGTTTAATTCTTCATG TTCTAGTCTCTCTCTCTCTCTCTCTCTCAAGTAATCTATCATAAGGTTCTCTTTTGAACTGAATTTGTGC TTAAGTTGTCTTCACTTTTATTATCTACAAATAAGCTAATGGGTGTGACGGCTTTCATAGCTGGCAGTAT TTACATGTGTGTAGAATGTATATATATAGTATCAGACATGCATGGAGCTTGGACCTTCTCCCTTAGAAAA TGCAGACGTGAATCAAACACCTTTTAGTCACTTACACAAATTTGCAAGTATAGTTCTGCTCATTATAAGG CAAACTGCTAACAATGTAAATTGTTCTGCTTAACAAAACCGTGAGTAACAGTGTCCCAGTATTCTTTCGT GAAGGTTAAAATCAGTGGGTTTGTCTTGTTGCCTGTGAAGCAATTCACAAACTCTCTTGTCAGCTTCCTT TTAGCTTTCAAAAAACATCTCATCTGATTGTGTCCTTTTGAGGAGACTGTTTCCATGCTAGGAAGGTCTG GCACCACACTAACTTGTAATTTAATATGCAAGACAAGCAATTTAAGAGTCTCATCGAACTAAATAAGTTC CAAACCTAGCTGGGCAATGGGGACACATGCCTTTAATCCCAGCACTCGGTGGGTAGAGGCAGGCTGATCT CTGTGAGTTTGAGGCCAGCCTGGTCTACAGAATGAGTTCCAGGACAGCAAGGGATATGCTGAGAAACTCT GTCTGGAAAAATCAAAGGAAAAAGTGTTTAAAAGTTTCAACCCTACGACCAAGAGCATAAAGTCTACAGA GGAATATGAACTGTACATGGTGCTGCCCCCTAAAGGACTCAAATCATTTAAGATATTCCTTTGATGAAAG ACCACTGCCTGTTTGCACACCTCTTTGGAGCATTTCAGTGACAAAGAGCTGGCTTGTGGTGAGTGTCTCT CTAAACTTTAATGAACTACTTTACACACAGTTATCCTTCTTCTACATCCCATCTCAGAAACGTGAAAAGC ATAGCACCTGGTACTACGCCTCTGGCAGGTGTCTATATTATGTGGAAACTTTTATGGAGTGATAGGACTA ATGGAAGACCTCAAAATTTGTATTTTCACTCTGTTATGCACTAGTACAAGTGTAGAGCTTGATTCTTTAG ACAATGCCTTTTTCTTATTTCTCATCCCCAATAAAGATCTCCCAGTGAGATTTTGTGTAAATAATCTGTA ATATAGGAATTCAGAGGGCCTCAGTCATTTATCACAGCTAGATGACCTTACAGACAGCAGAAAGGACAGG AAAGATAGTGGGAGACAGTAGTTTTCCTCACAAATTCTTACATTCCAGCCAAGACAAAGGATGTGTGAAG TCTGAGTTGGTACTGTTCCTGCAGAATGGTAGGAGCAGGTGGGATCTCCTGATACAATCTGGAACAAAGT TTGGCCTGTCACATGAGGTTTTGTTTACCAGGCTGTCTTGAAATGTCTGGGCAGATGGGTGTCTGCCAAA GCAAGGACTGATCCTGTCTGTGAACAGCAATGTCTCTGCTCTTCTTCTCTGTGGAGTGACTCACCCATTT ACTGACCACTTGTGAAATTGTCTAAAGATGTATACTGTAAAAGCTTCGAAAGAACTGTACAAACTGTAAG ATATTTTATACCTATTTTATGTTTTCTGTGTCTCATATTTATATCATAAAAACATTGGATCCCTAAAAAA AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6831137 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6831137 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14769 same embryo
	EMAGE:14768 same embryo
	EMAGE:14767 same embryo
	EMAGE:14766 same embryo
	EMAGE:14765 same embryo
	EurExpress:euxassay_005719 same experiment
	MGI:4827629 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source