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EMAGE:14783

B2m beta-2 microglobulin ( MGI:88127)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14783 EMAGE:14783 EMAGE:14783 EMAGE:14783 EMAGE:14783
"Pseudo-wholemount" of euxassay_005764. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005764_01 euxassay_005764_02 euxassay_005764_03 euxassay_005764_04
EMAGE:14783 EMAGE:14783 EMAGE:14783 EMAGE:14783 EMAGE:14783
euxassay_005764_05 euxassay_005764_06 euxassay_005764_07 euxassay_005764_08 euxassay_005764_09
EMAGE:14783 EMAGE:14783 EMAGE:14783 EMAGE:14783 EMAGE:14783
euxassay_005764_10 euxassay_005764_11 euxassay_005764_12 euxassay_005764_13 euxassay_005764_14
EMAGE:14783 EMAGE:14783 EMAGE:14783 EMAGE:14783 EMAGE:14783
euxassay_005764_15 euxassay_005764_16 euxassay_005764_17 euxassay_005764_18 euxassay_005764_19
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euxassay_005764_20 euxassay_005764_21 euxassay_005764_22 euxassay_005764_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14783Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14783_wholemount_strong.wlz
14783_wholemount_moderate.wlz
14783_wholemount_weak.wlz
14783_wholemount_possible.wlz
14783_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14783_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T9315
Entity Detected:B2m, beta-2 microglobulin ( MGI:88127)
Sequence:sense strand is shown

>T9315
GTCAGCATGGCTCGCTCGGTGACCCTGGTCTTTCTGGTGCTTGTCTCACTGACCGGCCTGTATGCTATCC
AGAAAACCCCTCAAATTCAAGTATACTCACGCCACCCACCGGAGAATGGGAAGCCGAACATACTGAACTG
CTACGTAACACAGTTCCACCCGCCTCACATTGAAATCCAAATGCTGAAGAACGGGAAAAAAATTCCTAAA
GTAGAGATGTCAGATATGTCCTTCAGCAAGGACTGGTCTTTCTATATCCTGGCTCACACTGAATTCACCC
CCACTGAGACTGATACATACGCCTGCAGAGTTAAGCATGCCAGTATGGCCGAGCCCAAGACCGTCTACTG
GGATCGAGACATGTGATCAAGCATCATGATGCTCTGAAGATTCATTTGAACCTGCTTAATTACAAATCCA
GTTTCTAATATGCTATACAATTTATGCACGCAGAAAGAAATAGCAATGTACACATCACCTTCTTTATATC
TTACTTTAAATATTTTATGCATGTTTTCAAAAAATTGGAAATATCCTAGATAGCTGAGCAATAAATCTTC
AATAAGTATTTTGATCAGAATAATAAATATAATTTTAAGAACAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
Notes:The probe template was PCR amplified from IMAGE:6846389 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6846389 which was retrieved directly from NCBI.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14782 same embryo
 EMAGE:14787 same embryo
 EMAGE:14785 same embryo
 EMAGE:14784 same embryo
 EMAGE:14786 same embryo
 EurExpress:euxassay_005764 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS