EMAGE mouse gene expression spatial database

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EMAGE:14784

Prokr2 prokineticin receptor 2 ( MGI:2181363)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005767. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005767_01	euxassay_005767_02	euxassay_005767_03	euxassay_005767_04

euxassay_005767_05	euxassay_005767_06	euxassay_005767_07	euxassay_005767_08	euxassay_005767_09

euxassay_005767_10	euxassay_005767_11	euxassay_005767_12	euxassay_005767_13	euxassay_005767_14

euxassay_005767_15	euxassay_005767_16	euxassay_005767_17	euxassay_005767_18	euxassay_005767_19

euxassay_005767_20	euxassay_005767_21	euxassay_005767_22	euxassay_005767_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14784_wholemount_strong.wlz

14784_wholemount_moderate.wlz

14784_wholemount_weak.wlz

14784_wholemount_possible.wlz

14784_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14784_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

vibrissa

moderate

regional

moderate expression: see section 07 18

thalamus

moderate

regional

moderate expression: see section 10 12 13 weak expression: see section 08 14 15

nasal cavity

strong

spotted

strong expression: see section 12 14 moderate expression: see section 11

pharynx

moderate

regional

moderate expression: see section 13 14 weak expression: see section 15

tongue

moderate

regional

moderate expression: see section 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T8792
Entity Detected:	Prokr2, prokineticin receptor 2 ( MGI:2181363)
Sequence:	sense strand is shown >T8792 CTGATGCGAGGAGAGCAGGACCAAGCGACACACGCCCACCAAGTAGGCAAGCCTCAACCAGAGCTAGTGT TCCCAGAGCCCGGGCCGCGCCCACCTATGGTGGCGCCACCGGCCAGGGAGTGCGTCCCTGAAAGCACCGA GGCAAATCCTGGATGGTATCTGTTCTCCCTAAGGATTCTAACAGGGACCTGCACTCACTGACCCCAGCAG AACTGCTGAACTCCACGTGAGCGCATCTCCCTGATACACACCAGCCCACCTGTAGCATCATCAACATGGG ACCCCAGAACAGAAACACTAGCTTTGCACCAGACTTGAATCCACCCCAAGACCATGTCTCCTTAAACTAC AGTTATGGTGATTATGACCTCCCCCTGGGTGAGGATGAGGATGTGACCAAGACACAGACCTTCTTTGCAG CCAAAATTGTCATTGGCGTGGCACTGGCAGGCATCATGCTGGTCTGCGGCATTGGCAACTTTGTCTTCAT TGCTGCCCTCGCCCGCTACAAGAAGCTGCGCAACCTTACCAACCTCCTCATTGCTAACCTGGCCATCTCT GACTTCCTGGTGGCGATCGTCTGCTGCCCCTTTGAGATGGACTATTATGTAGTACGGCAGCTTTCCTGGG CGCATGGTCACGTGCTTTGTGCCTCCGTCAACTACCTTCGTACGGTCTCCCTGTACGTCTCCACCAACGC TCTGCTGGCCATCGCTATTGACAGATACCTCGCTATTGTCCACCCTTTGAAACCACGGATGAATTATCAG ACCGCTTCCTTCCTGATCGCTTTGGTCTGGATGGTCTCCATCCTCATCGCTGTCCCATCTGCCTACTTCA CCACAGAAACCATCCTCGTTATCGTCAAGAATCAAGAAAAAATCTTCTGTGGTCAGATCTGGTCGGTGGA CCAGCAGCTCTACTACAAATCCTACTTCCTCTTCGTCTTCGGGCTTGAGTTCGTGGGTCCCGTGGTCACT ATGACCCTGTGCTATGCCAGGATCCCCCAAGAGCTCTGGTTCAAGGCTGTACCTGGCTTCCAGACGGAGC AAATCCGCAAGCGGCTGCGTTGCCGCCGCAAGACAGTGCTACTGCTCATGGGCATCCTCACAGCCTACGT GCTGTGCTGGGCGCCGTTCTATGGCTTTACCATAGTGCGAGACTTCTTCCCCACGGTAGTTGTGAAGGAG AAGCACTACCTCACCGCCTTCTACGTCGTGGAGTGCATTGCCATGAGCAACAGCATGATCAATACTATAT GCTTCGTGACGGTCAAGAACAACACCATGAAATACTTCAAGAAGATGCTGCGGCTCCACTGGCGGCCCTC TCACTACGGGAGTAAGTCCAGCGCTGACCTCGACCTCAAAACCAGCGGGGTGCCTGCCACTGAAGAGGTG GATTGTATCAGACTAAAGTAGCCTTCAGGTGTTGCCCAAGGAAAAATTTAACATTCGGTACTCAGTAAAT CACACACCATCAACCACTCACAAGCTACATGGAAAGATACGGCTGTATTCACGTTCTCCTGCTCTAATGT ATCAGGACGCTTCTATGTAATAACATACAGCACAACTGATGTCTGCATAACATCTTAGAAGGCAGACACA AATAGTAACAAGTGATGTGGACTGAATGCTTCTGTCTGCAAACCACACCAACCAATTATTCAAGGACAAG AGCTGACATGTGAGAATTACCTGCTATGTGCAAAAACAAGTTACCCCCCCAAAAAATGATAGAAGCTATT TGGAGTTATTCAGCTCTATCTATCTATCTATCTATCCATCCATCCATCCATCCATCCAGGTCACTAGAAA GAAGTCACAAATGACTAGCCAGAGTCATGCTACATATTCTTTCATTCTGTATCTTTTCTGCACAGAACTG TCAAAGGCAATAGAATAAAGCACCTAGACATACTAGAAATGTAAGGATAACTCCATCAATAGGGAGACCA AGGCCTCATAGGAAGAGGGTCCATATAGTATACTGACTTTCCCCACTCCACACCAGTTATCTCCTTAGAT ATTCTGTACTTATCTGCAATGATGTAATTTCAAATGAGGAAAAATAAGGGGACAGGCTTTACCACAGATG TATCAAATCTCATCAAGCCCATAGGGCAAAGATGGGAGGCTCCTGACACAAGAAATGTATCCAGTTCTGG ATAACTTTAATGCCAAGCATTTCAGGGCTCTGGGGTCTTGGAGGAAGAGGACACAGAAAGAGCCGAGGTT TCCAGTGGCAATGAGTATAATCTGTCCATTTGCTATGATTTGGACAATTTTCTAGAACATACTCCGACTT ACAAAAGGAACTCTACTTGAGATCCAAAGATCCGGGTAAAAGTCCTAACCCCAGGACTCATCTCTGTGTG TCTCCACTGTAATGAAATGGAAATAATGAAAACGGATCATTAGGAACATCAGCCCGGCGAAGTCATGGTG TGGATGTGATTTTCACCTCTTCCTTTGTGAAGAATGAGGTCGTGAAAAGCTCATTAGAGGGAGTTTGGAA TGGAGAAACAGCTCCACACTTTTCATCCCTCTTCTTTGAATCAGAGACCACTAAACTCATCTTTGAAGTA GCGTATCTATAGTGAGGCATAAAGGTCTCCCTGTCACAGAGTGCAATCAAGAAGATAGAGTCAATGCCCA TACCCTCAGCATCCCTGTGGTCTTAGACAGTCTTCCCAACAAAGCACTGGTGGACCCCAGGACTGAATTC ACTTGTATTATTATGTCATCTACTGAATACTAGGGTTGATCAGGTTAGCTAGATAGGTATTTTCTTCCTC CTTCACAGCCCCATATGTATCCCTCCCTTAAACTCAGTTACTAAGGAAGACCTTCTTAAACACAGGAGAA CCATTATTCTGTCCAGGACACAAATAACCTCTCCAGTAGACACTGTACCCTTCACATGTCAACAGAATTT GCCTCCTTCTTGTATTTAAACATATCATCCTCCTTTCATTTAGATTTAACCAGAAACCATTCCTGTAAAT TTCAATGTGTTTGTGATACCCCACTGTAAAAAGCGTATGCTGTTATCATATGGAATAATTAACATACAGA ATTGTAATCGTAGTTCCCAAAAGGTTCCCTACTCGTGTTGTATCTTATGTTTATATGTTTGATGTAAATG GAGCTGTGTAGCTGTCTAAGCAGCTCAAGCCTGAAATGAGGGAATGTCCAATGGTGTTCTTAGAGCAGGG CCATCTCAGGCTAGCAGCTGGCCTCAGTCTGTGCTCTCTCGGGAGTGTGTTCTTAAATATGAATTAGCAG CAAACCATTATCCTATGTGCTCCCTTGTTTATAAACGATTTTTAAAATGCAGGCTTCTTTTCACTTAGCG AATATTTGTGCGTCTGGCTAGAACTAAGGTAATAAGTTAATTACATTTTTCAGTCTTCCCAAGACTAATT AAGGGTAAGATTAAGACACTCTTAACACGTCAAGATACGTACACACTTATGCCTCCTATTTCATGGAGAA AGTTAATATCACTTAGCAAAGCCAGATCTATTTTCTTGGCCACATTATGAGAAAAGATGGTTCGACAATG AAGCTGTTACAGATTTTTGTTATGCATCCAACATTTAAAATAAGTTACTTCTAAATTTGGAGAAATCAAA AAAAAAAAAAAAAAA
Notes:	The probe template was PCR amplified from IMAGE:6408655 using vector (pYX-Asc) specific primers. Forward Primer - name:RZPD T3, sequence:ATTAACCCTCACTAAAGGGA; Reverse Primer - name:RZPD T7, sequence:TAATACGACTCACTATAGGG. Anti-sense probe was then transcribed from the PCR amplified template using T7 polymerase. EMAGE Editor's Note: the probe sequence indicated here was given by the EURExpress Consortium and has been checked using a computational method whereby a BLAST comparison was made against the full insert sequence of IMAGE:6408655 which was retrieved directly from NCBI.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14782 same embryo
	EMAGE:14783 same embryo
	EMAGE:14787 same embryo
	EMAGE:14785 same embryo
	EMAGE:14786 same embryo
	EurExpress:euxassay_005767 same experiment
	MGI:4827437 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source