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EMAGE:14844

Zfhx4 zinc finger homeodomain 4 ( MGI:2137668)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14844 EMAGE:14844 EMAGE:14844 EMAGE:14844 EMAGE:14844
"Pseudo-wholemount" of euxassay_005820. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005820_01 euxassay_005820_02 euxassay_005820_03 euxassay_005820_04
EMAGE:14844 EMAGE:14844 EMAGE:14844 EMAGE:14844 EMAGE:14844
euxassay_005820_05 euxassay_005820_06 euxassay_005820_07 euxassay_005820_08 euxassay_005820_09
EMAGE:14844 EMAGE:14844 EMAGE:14844 EMAGE:14844 EMAGE:14844
euxassay_005820_10 euxassay_005820_11 euxassay_005820_12 euxassay_005820_13 euxassay_005820_14
EMAGE:14844 EMAGE:14844 EMAGE:14844 EMAGE:14844 EMAGE:14844
euxassay_005820_15 euxassay_005820_16 euxassay_005820_17 euxassay_005820_18 euxassay_005820_19
EMAGE:14844 EMAGE:14844 EMAGE:14844 EMAGE:14844 EMAGE:14844
euxassay_005820_20 euxassay_005820_21 euxassay_005820_22 euxassay_005820_23 euxassay_005820_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14844Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14844_wholemount_strong.wlz
14844_wholemount_moderate.wlz
14844_wholemount_weak.wlz
14844_wholemount_possible.wlz
14844_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14844_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Text Annotation:
StructureLevelPatternNotes
hand epidermis
moderate moderate
regionalmoderate expression: see section 01 02 03 04 23 24
foot epidermis
moderate moderate
regionalmoderate expression: see section 02 03 04 05 06 07 19 20 21 22 23 24
medulla oblongata basal plate mantle layer
strong strong
regionalstrong expression: see section 07 08 14 15
nasal septum
moderate moderate
regionalmoderate expression: see section 13 14 15 16 17 18 weak expression: see section 11 12
urethra of male
moderate moderate
regionalmoderate expression: see section 11 12 13 14
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35564
Entity Detected:Zfhx4, zinc finger homeodomain 4 ( MGI:2137668)
Sequence:sense strand is shown

>T35564
TGCTTCTGGCCTAGATGGTAATTTCAATAGTGTCCGAATGGATATGTTCAGTGTGTAGGAGTGAAGACAG
GATCCCGTGCTTAAAAAAAAATTTAAAAATAAAAAATTTTTAAAAAAAGACTTTAACTGCAGTTCCAAAG
CTTCTCTAACCCAAAAATTACAGTACCAAATGATTGGCTCAGGATTGTTTTTCCCATATTGATATGCTGG
CAATATAGGATGGTATGTGGTGAAGAAGACTAATGCAGGTGGTTGAATGCGCTTGTAATATATGCTAAAA
TATGGAAAAGGAAAAAAATATCTCACAAGTTCTTTGGGAACTTGTTTCAAGCCAAAAACTCTCAAGACAG
AAAATTGCACCTCAGCTGGGTTGATTTCAAAATGCCAGCATGGACAGTATGGGAGGACGATCCGGATGTG
TCAAAGAGAACTTCTCTTAGTTTAGTTAGGTGTAATTCAGTAGCTTTAAATTCTCAGGTCAGAACATAAC
ATTTCTCATTTGTTAAAAGCAGCAAGAAGCCTGGTAAAACTGTGACTTTTCCCCAAATGTCAATCTTTAT
TAGAAAGCATTTTCTAGGTGTGTTTAGTGTGCAAAGAAACTTTCTAACCCTTACCGGACACCGCACAGCT
TCCTGCACTCAAGCCGCAGGGTAGTACAGTTTTACCACAGAGGGACTCTTGAGCAATGGAGTCACGTGTC
TGCCCTGTGTATTGCAGTTTGTCTTGACACAAGC
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 91784. Forward Primer - name:091784_F_cDNA_Zfhx4, sequence:TGCTTCTGGCCTAGATGGTAAT; Reverse Primer - name:091784_N_SP6_cDNA_Zfhx4, sequence:GCTTGTGTCAAGACAAACTGCAA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14848 same embryo
 EMAGE:14845 same embryo
 EMAGE:14846 same embryo
 EMAGE:14843 same embryo
 EMAGE:14847 same embryo
 EurExpress:euxassay_005820 same experiment
 MGI:4829288 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS