EMAGE mouse gene expression spatial database

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EMAGE:14852

Smad1 MAD homolog 1 (Drosophila) ( MGI:109452)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_005811. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_005811_01	euxassay_005811_02	euxassay_005811_03	euxassay_005811_04

euxassay_005811_05	euxassay_005811_06	euxassay_005811_07	euxassay_005811_08	euxassay_005811_09

euxassay_005811_10	euxassay_005811_11	euxassay_005811_12	euxassay_005811_13	euxassay_005811_14

euxassay_005811_15	euxassay_005811_16	euxassay_005811_17	euxassay_005811_18	euxassay_005811_19

euxassay_005811_20	euxassay_005811_21	euxassay_005811_22	euxassay_005811_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14852_wholemount_strong.wlz

14852_wholemount_moderate.wlz

14852_wholemount_weak.wlz

14852_wholemount_possible.wlz

14852_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14852_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 16 weak expression: see section 15

glossopharyngeal ix ganglion

weak

regional

weak expression: see section 06 07 17

trigeminal v ganglion

weak

regional

weak expression: see section 03 04 05 06 07 08 17 18 19 20 21

vestibulocochlear viii ganglion

weak

regional

weak expression: see section 06 07 17

dorsal root ganglion

weak

regional

weak expression: see section 06 07 08 09 10 11 12 13 15 16 17

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T35535
Entity Detected:	Smad1, MAD homolog 1 (Drosophila) ( MGI:109452)
Sequence:	sense strand is shown >T35535 TGCATGTACTTGAAGGATGGACAAGTCAGACAGGATGGAGACCTGACGAAGGAGCCACGATAATACTTGA CCTCTGTGACCAACTATTGGATTGAGAAACTGACAAGCCTTGGTTGATAGCAAGAACCCTTTCAGTTTAC ATTGTGACATTCTGTTGTAAAAATCAACTAAAATGCTGACTTTCAGCAGGACTTTTGTGTATAGTTAAAA AAAAAAGAGATGGCCAAGCCAGGGACAAATTATCTATTAGGAAAAAAGAAAAAAATGATTGTAATCAATC CTTTTGTGTGGGGTGTTGGCAGAAGGTTGGCGCTGATAGTCTTTCTGAAGTGGGCTTTCATCAGGCTCAG AGCCCACGTTGAATCATCTTCTCATGGGTTTTCTTAATATTTTAAAACTACTTGTTTAGAAATGAATGGG TTTTTTGTTTGTTTTTAAAGTACAGGTTAATCGTTATGACATGCATAGTAATCTTTCTGAAACTGTATGC TGGCTGTATTACTGTCAGAATGATGGCAGGCATATGCTCTTTGCTAAATATGTATATACAGAATATTTGG AGGTTATGAATAGTCTAAATGGCTAGTGGGTTTACAGAGTATCTGAGGGGCGGGGTCGGGAAGAAAACGA CGGCTGCAAATGTAGACTATACCGTAAAGCTCAGCTTGCTGCCTTAAACAGACAAGCTGGTGTCTGAATT TGCTGTGTTTCAGTTTTTGTAGAGTTTTATCTGACTTCTTTTCTTCTGTCTTATCCGCTCCACGGCACAG TTAAGCAGCTGGTTAATTCCTCTAACTGTGAGAGCAGATGAGTAATTCCTTCTGTTCGCAAATCAACTGG CTTCGTGTTTCAGTACCCAGTAT
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 96594. Forward Primer - name:096594_F_cDNA_Smad1, sequence:TGCATGTACTTGAAGGATGGAC; Reverse Primer - name:096594_N_SP6_cDNA_Smad1, sequence:ATACTGGGTACTGAAACACGAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14850 same embryo
	EMAGE:14849 same embryo
	EMAGE:14851 same embryo
	EMAGE:14853 same embryo
	EurExpress:euxassay_005811 same experiment
	MGI:4828312 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source