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EMAGE:14855

Sox7 SRY-box containing gene 7 ( MGI:98369)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14855 EMAGE:14855 EMAGE:14855 EMAGE:14855 EMAGE:14855
"Pseudo-wholemount" of euxassay_005812. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_005812_01 euxassay_005812_02 euxassay_005812_03 euxassay_005812_04
EMAGE:14855 EMAGE:14855 EMAGE:14855 EMAGE:14855 EMAGE:14855
euxassay_005812_05 euxassay_005812_06 euxassay_005812_07 euxassay_005812_08 euxassay_005812_09
EMAGE:14855 EMAGE:14855 EMAGE:14855 EMAGE:14855 EMAGE:14855
euxassay_005812_10 euxassay_005812_11 euxassay_005812_12 euxassay_005812_13 euxassay_005812_14
EMAGE:14855 EMAGE:14855 EMAGE:14855 EMAGE:14855 EMAGE:14855
euxassay_005812_15 euxassay_005812_16 euxassay_005812_17 euxassay_005812_18 euxassay_005812_19
EMAGE:14855 EMAGE:14855 EMAGE:14855 EMAGE:14855 EMAGE:14855
euxassay_005812_20 euxassay_005812_21 euxassay_005812_22 euxassay_005812_23 euxassay_005812_24

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14855Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14855_wholemount_strong.wlz
14855_wholemount_moderate.wlz
14855_wholemount_weak.wlz
14855_wholemount_possible.wlz
14855_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14855_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35536
Entity Detected:Sox7, SRY-box containing gene 7 ( MGI:98369)
Sequence:sense strand is shown

>T35536
TGATGTCCTCTGTTTCTGGATGTCCCCCATCTCCAGCCTATTACTCCCATGCCACCTACCACCCTCTCCA
CCCCAACCTCCAGGCCCACCTGGGCCAGCTGTCCCCACCTCCGGAGCACCCTGGCTTTGACACCTTGGAT
CAGCTAAGCCAGGTGGAACTTCTGGGAGACATGGATCGCAATGAATTTGATCAGTATTTGAACACTCCCG
GCCACCCTGACTCTGCTGCAGGGGTTGGAACCCTCACTGGGCATGTCCCGCTCTCCCAGGGGACTCCCAC
AGGCCCTACAGAGACCAGCCTCATCTCAGTCCTGGCTGATGCCACGGCCACGTATTACAACAGCTACAGT
GTGTCATAGAGCTGGAGGAATGGAGCCTGGCCCAGCCCTGCCATCCCCTCCTCCCTATGAAGCACTGAGC
TGAGCAGAAACTGTGGCGATGCCACTCCTTGGTGGCAAGAGGTCAAAACTGCTGTTCCAGAGCCTTCCGG
TCAAAAGCCAGGATCCAGCGCTCCTTCTGTCCCTTTAAATGTGTTTTGTCGGCTTCATCAAGGAAGCACA
GCCTTGGGAGCTGTTTGCAGTAGGATTTCCTAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 90401. Forward Primer - name:090401_F_cDNA_Sox7, sequence:TGATGTCCTCTGTTTCTGGATG; Reverse Primer - name:090401_N_SP6_cDNA_Sox7, sequence:CTAGGAAATCCTACTGCAAACAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14858 same embryo
 EMAGE:14854 same embryo
 EMAGE:14856 same embryo
 EMAGE:14859 same embryo
 EMAGE:14857 same embryo
 EurExpress:euxassay_005812 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS