Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:149

En2 engrailed 2 ( MGI:95390)
TS17 (10.5 dpc)
in situ hybridisation

Data Images
EMAGE:149
Figure 3B of Liu et al., 1999 [PMID:10518499] . Copyright: This image is from Development and is displayed with permission of The Company of Biologists Ltd. who owns the copyright.

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Notes:
The green arrowhead points to the expression domain in the mesencephalon/metancephalon
Expression Pattern Description
Spatial Annotation:
EMAGE:149Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
149_wholemount_strong_3D_1.wlz
149_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:149_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
hindbrain
detected detected
regionalExpression is in a wide band covering the caudal part of the mesencephalon and the metencephalon.
midbrain
detected detected
regionalExpression is in a wide band covering the caudal part of the mesencephalon and the metencephalon.
Annotation Validation: EMAGE Editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:MGI:1346822
Entity Detected:En2, engrailed 2 ( MGI:95390)
Sequence:sense strand is shown

>MGI:1346822
AGATCTTTCTTTCATTTTTCTTTTCTCCCTTTTTTTAATCCCTTAAATTCCATTATGGGACATTGGACAC
TTCTTCCCCCGAAAGAAAAAAAATTTAAAACAACTTGCTGAAGTCCAAAGATTTTTATTGCTGCATTTCA
CAGGACTGTGAACCGAATAAATAGCTCCTATTTGGTCTGTGGGCTCTGCCGCTTGCTTTGTGCTGGCTCG
GACAGCACTGTAGGAAGCGCCAGCCAGAGTGGTGATGGAGAGCTCTGGTGGGGAGAGAGGGCGCAGTTCT
TTGCTGAGGTCTGAGAAAGTCCTTGCAGGCCAGCAGCTCTCCCGGAACCTCTCCGCCTGCTTCCTACGCA
GCCTCTCTTCTCAACCATCTCAGAGGCCCGTGCTGGCAGCCACCGTGTGTCTGCGTCTGTGTCGATGACC
CCTGTCCGCCACAGCCCCGTAGCGACCTGCCAGCGCCAACTCTCCCGCCACCGTGGCCAGCGTTGGGGCC
TGGGGAGCAGTGGGAGTGTGTCCTGAAGGCTTGAAGGCTTTGCTGCCGGGACAGCTAGCCCTGACCCAAA
AGCCCTCACCGAGATAGGCCTTGGGGCCTGGCAGAGCGAGTTGGAATCATCTTCCTTTCCTTTTTCTTCC
CTCTTTTTTCTTTTTCATTTATTTTTGAATGGTAAATACATGTATGTGTACATACTTGTGTATGCATGTG
CACGCACACCCCAGGCCTGTGTGAAGCTGGGGTTCTGGAGCTCACAGCTACCTTGACTTTCAAAGGAACT
CGAATCCTCCCGAAT
nt 2105 - nt 2889 of NM_010134.2
Notes:The probe used in this study, Liu et al., 1999 [PMID:10518499] is indicated as being originally described by Millen et al., 1995 [PMID:8575294] ie. as "a 800 bp BglII-XbaI, 3' untranslated fragment". Editors Note: Digestion of the En2 mouse RefSeq mRNA sequence NM_010134.2 with BglII and XbaI identifies a 784nt 3'-UTR fragment (co-ordinates 2106-2889 as shown above).
Chemistry:RNA
Strand:antisense
Specimen
Organism:mouse
Strain:Swiss
Age:10.5 dpc
Theiler Stage:TS17
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
General Information
Authors:Liu et al., 1999 [PMID:10518499] . Indexed by GXD, Spatially mapped by EMAGE.
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:non-screen
References:[ PMID:10518499] Liu A, Losos K, Joyner AL 1999 FGF8 can activate Gbx2 and transform regions of the rostral mouse brain into a hindbrain fate. Development (126):4827-38
 [ PMID:8575294] Millen KJ, Hui CC, Joyner AL 1995 A role for En-2 and other murine homologues of Drosophila segment polarity genes in regulating positional information in the developing cerebellum. Development (121):3935-45
Links:MGI:1349176 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceMGI