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EMAGE:14908

B3galt1 UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 ( MGI:1349403)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14908 EMAGE:14908 EMAGE:14908 EMAGE:14908 EMAGE:14908
"Pseudo-wholemount" of euxassay_007952. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007952_01 euxassay_007952_02 euxassay_007952_03 euxassay_007952_04
EMAGE:14908 EMAGE:14908 EMAGE:14908 EMAGE:14908 EMAGE:14908
euxassay_007952_05 euxassay_007952_06 euxassay_007952_07 euxassay_007952_08 euxassay_007952_09
EMAGE:14908 EMAGE:14908 EMAGE:14908 EMAGE:14908 EMAGE:14908
euxassay_007952_10 euxassay_007952_11 euxassay_007952_12 euxassay_007952_13 euxassay_007952_14
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euxassay_007952_15 euxassay_007952_16 euxassay_007952_17 euxassay_007952_18 euxassay_007952_19
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euxassay_007952_20 euxassay_007952_21 euxassay_007952_22 euxassay_007952_23

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14908Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14908_wholemount_strong.wlz
14908_wholemount_moderate.wlz
14908_wholemount_weak.wlz
14908_wholemount_possible.wlz
14908_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14908_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35992
Entity Detected:B3galt1, UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 1 ( MGI:1349403)
Sequence:sense strand is shown

>T35992
CTTCAAAGGTCTCCTGCCTCTATGTATTGTCCGTTGTGTGCTGGGCCAGCGCTCTCTGGTACTTGAGCAT
AACCAGACCTACTTCATCCTACACTGGCTCCAAGCCGTTCAGTCACCTAACAGTTGCCAGGAAAAACTTC
ACCTTTGGCAACATAAGAACTCGACCTATAAACCCACATTCTTTTGAATTTCTGATAAATGAGCCCAACA
AGTGCGAGAAAAACATTCCTTTTCTCGTGATCCTCATTAGCACCACACACAAGGAATTTGATGCCCGCCA
GGCAATCCGGGAGACATGGGGGGATGAAAACAACTTCAAAGGGATCAAGATAGCCACACTTTTCCTCCTG
GGCAAAAATGCTGATCCTGTTCTGAACCAGATGGTGGAGCAAGAGAGCCAGATCTTCCATGACATCATCG
TGGAGGACTTCATTGACTCCTACCACAATCTCACCCTCAAAACCTTAATGGGGATGAGATGGGTTGCCAC
TTTCTGTTCAAAAGCCAAGTACGTCATGAAAACCGACAGTGACATTTTTGTGAACATGGACAACCTTATT
TATAAACTCCTGAAACCCTCTACCAAGCCAAGAAGAAGGTATTTCACTGGTTACGTCATCAACGGCGGGC
CAATCAGGGATGTCCGCAGTAAGTGGTATATGCCTAGAGATTTGTACCCTGACAGCAACTACCCACCGTT
CTGTTCAGGGACTGGCTATATCTTTTCCGCTGATGTGGCTGAACTCATTTACAAGACCTCGCTCCACACC
AGGCTGCTTCATCTT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74967. Forward Primer - name:074967_F_cDNA_B3galt1, sequence:CTTCAAAGGTCTCCTGCCTCTA; Reverse Primer - name:074967_N_SP6_cDNA_B3galt1, sequence:AAGATGAAGCAGCCTGGTGTG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14909 same embryo
 EMAGE:14906 same embryo
 EMAGE:14905 same embryo
 EMAGE:14904 same embryo
 EMAGE:14907 same embryo
 EurExpress:euxassay_007952 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS