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EMAGE:14931

Tmem74 transmembrane protein 74 ( MGI:2443417)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:14931 EMAGE:14931 EMAGE:14931 EMAGE:14931 EMAGE:14931
"Pseudo-wholemount" of euxassay_007960. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_007960_01 euxassay_007960_02 euxassay_007960_03 euxassay_007960_04
EMAGE:14931 EMAGE:14931 EMAGE:14931 EMAGE:14931 EMAGE:14931
euxassay_007960_05 euxassay_007960_06 euxassay_007960_07 euxassay_007960_08 euxassay_007960_09
EMAGE:14931 EMAGE:14931 EMAGE:14931 EMAGE:14931 EMAGE:14931
euxassay_007960_10 euxassay_007960_11 euxassay_007960_12 euxassay_007960_13 euxassay_007960_14
EMAGE:14931 EMAGE:14931 EMAGE:14931 EMAGE:14931 EMAGE:14931
euxassay_007960_15 euxassay_007960_16 euxassay_007960_17 euxassay_007960_18 euxassay_007960_19
EMAGE:14931 EMAGE:14931
euxassay_007960_20 euxassay_007960_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:14931Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
14931_wholemount_strong.wlz
14931_wholemount_moderate.wlz
14931_wholemount_weak.wlz
14931_wholemount_possible.wlz
14931_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:14931_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T35991
Entity Detected:Tmem74, transmembrane protein 74 ( MGI:2443417)
Sequence:sense strand is shown

>T35991
CTGTAAAGGGAATGAGGACCAGAGGAGCAGGCCTCCCTACAGTCCGGGATTAAATTCTGCCAGATTCTTC
TTATCTAATTGTGTTGCTGATAGCAAAAGTTTGGAAATTTCGGGGAGAAATCTATCCAGCTCCCATGGAG
CTCCACTCTCTGTCTAAGAGGAACAGCCCAGTGGACCCATGCAATGCTTTGGAGTGGAGTTCAGGAGAGA
CTTCTGGTGACCATATAGAAGAGGCAACCATCAGAGATGCTTTTTGCTATCAGAAAAACTTGGTATCAAC
ACCAAGGGCAGATGTGGTGGAAGTGTGTAGACTTAGCACTTCTCCAGCATCCCCAACCTCCTTGCTCCAA
GACAGTGCTATTCAAACAAGCTTCTCCCTATCTGGACCTCCTGACTCAGGGAACAACCAAGTAATGGCTG
ATCGCAAAGTCTGTAATTGTTGCAGCCAGGAACTAGAAACTTCTTTTACTTACGTGGATGAGAACGTCAA
CTTGGAACAGAGAAGTCAGAGGTCACCATCAGCCAAAGGCAGTAATCACCCTGTAGATCTGGGATGGGGA
AACCCTAATGAATGGTCTCATGAAACTGCCATGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 68782. Forward Primer - name:068782_F_cDNA_B230382K22Rik, sequence:CTGTAAAGGGAATGAGGACCAG; Reverse Primer - name:068782_N_SP6_cDNA_B230382K22Rik, sequence:ACATGGCAGTTTCATGAGACCAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:14928 same embryo
 EMAGE:14930 same embryo
 EMAGE:14929 same embryo
 EMAGE:14932 same embryo
 EMAGE:14927 same embryo
 EurExpress:euxassay_007960 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS