EMAGE mouse gene expression spatial database

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EMAGE:14977

Lrrc16b leucine rich repeat containing 16B ( MGI:2448573)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008003. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008003_01	euxassay_008003_02	euxassay_008003_03	euxassay_008003_04

euxassay_008003_05	euxassay_008003_06	euxassay_008003_07	euxassay_008003_08	euxassay_008003_09

euxassay_008003_10	euxassay_008003_11	euxassay_008003_12	euxassay_008003_13	euxassay_008003_14

euxassay_008003_15	euxassay_008003_16	euxassay_008003_17	euxassay_008003_18	euxassay_008003_19

euxassay_008003_20	euxassay_008003_21	euxassay_008003_22	euxassay_008003_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

14977_wholemount_strong.wlz

14977_wholemount_moderate.wlz

14977_wholemount_weak.wlz

14977_wholemount_possible.wlz

14977_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:14977_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hypothalamus mantle layer

moderate

regional

moderate expression: see section 09 10 11 12 13 14 15 16 17 18

diencephalon lateral wall mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18

telencephalon mantle layer

moderate

regional

moderate expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

medulla oblongata basal plate mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 16 17 18 weak expression: see section 06 07

metencephalon rest of alar plate mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 weak expression: see section 03 04

pons mantle layer

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 14 15 16 17 18 19

midbrain mantle layer

moderate

regional

moderate expression: see section 05 06 07 08 09 10 11 12 13 14 15 16 17 18 weak expression: see section 04

glossopharyngeal ix ganglion

moderate

regional

moderate expression: see section 06 07 18 19

trigeminal v ganglion

moderate

regional

moderate expression: see section 04 05 06 07 08 09 10 18 19 20 21 22

vagus x ganglion

moderate

regional

moderate expression: see section 08 17

vestibulocochlear viii ganglion

moderate

regional

moderate expression: see section 06 07 08 09 17 18 19

spinal cord mantle layer

moderate

regional

moderate expression: see section 08 09 10 11 12 13 14 15 weak expression: see section 16

dorsal root ganglion

moderate

regional

moderate expression: see section 06 07 08 09 10 11 12 13 16 17 18

nasal cavity olfactory epithelium

weak

regional

weak expression: see section 12 13 14 15 17 18 19 20

extraembryonic component

moderate

regional

moderate expression: see section 07

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36025
Entity Detected:	Lrrc16b, leucine rich repeat containing 16B ( MGI:2448573)
Sequence:	sense strand is shown >T36025 CTCGGAAACTTCCACATCTACCACTCACAGTGTGTGTGGTGGCTTCTCTGAGACCTACGCTGCCCTGTGT GACTACAATGGACTCCACTGCCGAGAGGAGGTGCAATGGGATGTGGACACCATCTACCATGCTGAGGATA ACCGAGAGTTCAATCTTTTGGATTTCAGCCACTTGGAGAGCCGAGATCTGGCCCTCATGGTGGCCGCCCT GGCCTACAACCAGTGGTTCACCAAACTCTACTGCAAAGACTTGAGACTGGGCTCAGAAGTGCTAGAACAG GTGCTACATACCCTCAGCAAGTCGGGGAGCCTCGAAGAGCTGGTGCTGGACAATGCTGGGCTTAAGACGG ACTTTGTCCAGAAGCTGGCCGGGGTGTTTGGGGAGAACGGGAGCTGTGTGCTGCATGCGCTCATTCTGTC CCACAACCCCATCGAGGACAAGGGTTTCCTCAGCCTGAGCCAGCAGCTCCTCTGCTTCCCTACGGGCCTC ACCAAACTGTGCCTGGCCAAGACTGCCATCTCTCCTCGAG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 84686. Forward Primer - name:084686_F_cDNA_BC036313, sequence:CTCGGAAACTTCCACATCTACC; Reverse Primer - name:084686_N_SP6_cDNA_BC036313, sequence:CTCGAGGAGAGATGGCAGTCTT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:14972 same embryo
	EMAGE:14975 same embryo
	EMAGE:14973 same embryo
	EMAGE:14976 same embryo
	EMAGE:14974 same embryo
	EurExpress:euxassay_008003 same experiment
	MGI:4825990 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source