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EMAGE:150

Alx3 aristaless-like homeobox 3 ( MGI:1277097)
TS18 (11.5 dpc)
in situ hybridisation

Data Images
EMAGE:150

Expression pattern clarity: three stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:150Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
150_wholemount_strong_3D_1.wlz
150_wholemount_possible_3D_1.wlz
150_wholemount_notDetected_3D_1.wlz
(what is wlz format?)
Download all expression domains: EMAGE:150_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: submitter
Detection Reagent
Type:in situ hybridisation probe
Identifier:Alx3 probeA
Entity Detected:Alx3, aristaless-like homeobox 3 ( MGI:1277097)
Sequence:sense strand is shown

>Alx3 probeA
CCATGGCTTCCCTCCTGCCCTGGGAGGGCACAGCTTTGAGCCTTCTCCGGATGGTGACTACAAGTCCCCA
AGCCTCGTTTCACTCAGGATGAAGCCCAAAGAGCCCCCTGGCCTGCTGAACTGGACCACGTGATGGGTTA
CATGGGCACAGACTGAGCTGTCCACTTCTCTTCCTGCTCTGACCTGCTCCTAGCCCATCTCTACCTGGAC
ACCAAACAGCAAACCCTTTTACCTCCATAACCTGGGTGGTTCTGGAAGCTTGGAAGAGGAGCTGGGACCC
TCAGCCTCCTGCAGGGAACAGGCTACAGGGTCTTATTTCTTAAAATAGGTGGGGCTGCCTAGGAGACAGG
GACTTCAAAGCAGGTAGGACTCCTCCTTATAAAATGGCTCCCCTCATTTAAATTGGGCTGGAGAAAAGGT
GACAGATGCCCCCTGTTTTCCAAAGACTGTTTCTAAGTCCAGGGAGCCCAGGAAGATGGGAGGAGGGTGC
AGACTAAACAGGGAGGCGCTCTCTGTGGTTCTTAGGGCTGGCCTCACTTTTGCCTTCCTACCACCAGGGG
GCGGTACGCTCAGCTCCTGGGACTGACAAGATCCCAGACGGTGAAGGTCAGCTCAATAATAGGTGGGGGG
GTCTCCTCTGTTCCAAGCATCCCTCACTTCTGCCCTGTGCTAGTCTACAGAGATACTATAAAGTCATTTT
CTCCAAGTAGGGAGAAGAAATTCCACCAAACATTAAATATTTGGATTTGCTTTCATGGGATCAGGGATAT
GCCATGAAAACCATATTGGACATCGATCAAAATAAAAAAAAAATTTTTTTTCC
nt 1048 - nt 1870 of NM_007441.1
Notes:The probe used in this study was synthesised from a 1.86kb Alx3 clone following internal linearisation using NcoI. The probe encompasses part of the coding region and the 3' UTR. (The entire template clone was originally described by ten Berge et al, 1998 [PMID:9676189] and has been indexed in the MGI clone and segments database as MGI:1277368),
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Age:11.5 dpc
Theiler Stage:TS18
Mutations:none (wild-type)
Preparation:wholemount
Procedures
Fixation:4% paraformaldehyde
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Kirsten Steiner, Meredith O'Rourke, Patrick Tam
Principal investigator:Patrick Tam, Children's Medical Research Institute, Embryology Unit, CMRI, Locked Bag 23 Wentworthville, Sydney, Australia 2145
Submitted by:Kirsten Steiner, Children's Medical Research Institute, Embryology Unit, CMRI, Locked Bag 23 Wentworthville, Sydney, Australia 2145
Experiment type:non-screen
References:[ doi:10.1006/dbio.1998.8921] [ PMID:9676189] ten Berge D, Brouwer A, el Bahi S, Guénet JL, Robert B, Meijlink F 1998 Mouse Alx3: an aristaless-like homeobox gene expressed during embryogenesis in ectomesenchyme and lateral plate mesoderm. Dev Biol (199):11-25
Links:MGI:1277368 Template DNA description
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEMAGE