EMAGE mouse gene expression spatial database

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EMAGE:15016

Extl1 exostoses (multiple)-like 1 ( MGI:1888742)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_008093. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_008093_01	euxassay_008093_02	euxassay_008093_03	euxassay_008093_04

euxassay_008093_05	euxassay_008093_06	euxassay_008093_07	euxassay_008093_08	euxassay_008093_09

euxassay_008093_10	euxassay_008093_11	euxassay_008093_12	euxassay_008093_13	euxassay_008093_14

euxassay_008093_15	euxassay_008093_16	euxassay_008093_17	euxassay_008093_18	euxassay_008093_19

euxassay_008093_20	euxassay_008093_21

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15016_wholemount_strong.wlz

15016_wholemount_moderate.wlz

15016_wholemount_weak.wlz

15016_wholemount_possible.wlz

15016_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15016_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

hindlimb digit 1 metatarsal

weak

regional

weak expression: see section 02 21

hindlimb digit 2 metatarsal

weak

regional

weak expression: see section 02

hindlimb digit 3 metatarsal

weak

regional

weak expression: see section 02

hindlimb digit 4 metatarsal

weak

regional

weak expression: see section 02 03

hindlimb digit 5 metatarsal

weak

regional

weak expression: see section 03 04

fibula

weak

regional

weak expression: see section 01 21

tibia

weak

regional

weak expression: see section 01 02 21

nasal septum

weak

regional

weak expression: see section 12 13 14

exoccipital bone

weak

regional

weak expression: see section 01 02 03 16 17 18 19

orbito-sphenoid

weak

regional

weak expression: see section 01 08 09 10 17 18 19

viscerocranium

weak

regional

Expression in the turbinate bone.

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T36356
Entity Detected:	Extl1, exostoses (multiple)-like 1 ( MGI:1888742)
Sequence:	sense strand is shown >T36356 CTATCAAGGTGCCTTATGGGAGGCAGCACCCGGAGGCTGTGCCAATGGATTCTGGGGATCCACGACCAGT GCCGGAGCCTCAGCCCCTGGATCAGGACTGCATCAACCGTTTAGCAGCAGGGTTCGGCCACATGCCTCTG GTTTCCTCTCAAGTGCGTCTGGATCCGGTGTTGTTCAAGGACCCGGTATCGGTGCAACGCAAGAAGTATC GCAGTCTAGAGAAGCCATAGGCGAGGCACTGCACCTGTCTGCTAGACACGAGAGAACCAACAGAGATGGA TCAAAGTCCTGTCCTGGTTGGCCAATCGCTACAGGGATCCCTGAGGGCCGGGGCCTTCTCCAGCTGTAGT GGGGTTGCGTTTGGCACGTTTCGCACAGGCCGGTGTACAGGTTGATCCTGTTCCCTGAAATGCCTTTCTC TGCATCTCTGCCTGGTCGACCTATGTCTGTCAGACCTGCACTCACCTCCTTGGTGATCTCGAAGTCCTTG CACGATTCACGCCGAGTGGCCTTGAGCACATCACGAGGCCCCAGGGCCGGCCTGCAAGCTCCGTAGGCGG AGTCCTGCTCTCATCTCTTTGGTCTGGCAGTGCACTGTAAATATTTGAGCAATAGCTGCAAGAGTATTAA CTGGTGGCATGCGTGGTGGAACACGGGCAGAATGTGCACAAGACATAACGCTGCCCCTTACTCTGGAGTT CCTGGTCCCGGTACAAAGCACACACCAGGATTTCCAGACTCAATCAAGCGTGAACAAAGAGGCCAGGAGT CGAAGATAACCAGAGGATTCGGTGTCTCGGTAGACCC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 100434. Forward Primer - name:100434_F_cDNA_Extl1, sequence:CTATCAAGGTGCCTTATGGGAG; Reverse Primer - name:100434_N_SP6_cDNA_Extl1, sequence:GGGTCTACCGAGACACCGAAT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15014 same embryo
	EMAGE:15018 same embryo
	EMAGE:15013 same embryo
	EMAGE:15017 same embryo
	EMAGE:15015 same embryo
	EurExpress:euxassay_008093 same experiment
	MGI:4824652 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source