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EMAGE:15020

Epb4.2 erythrocyte protein band 4.2 ( MGI:95402)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15020 EMAGE:15020 EMAGE:15020 EMAGE:15020 EMAGE:15020
"Pseudo-wholemount" of euxassay_008048. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008048_01 euxassay_008048_02 euxassay_008048_03 euxassay_008048_04
EMAGE:15020 EMAGE:15020 EMAGE:15020 EMAGE:15020 EMAGE:15020
euxassay_008048_05 euxassay_008048_06 euxassay_008048_07 euxassay_008048_08 euxassay_008048_09
EMAGE:15020 EMAGE:15020 EMAGE:15020 EMAGE:15020 EMAGE:15020
euxassay_008048_10 euxassay_008048_11 euxassay_008048_12 euxassay_008048_13 euxassay_008048_14
EMAGE:15020 EMAGE:15020 EMAGE:15020 EMAGE:15020 EMAGE:15020
euxassay_008048_15 euxassay_008048_16 euxassay_008048_17 euxassay_008048_18 euxassay_008048_19
EMAGE:15020 EMAGE:15020 EMAGE:15020
euxassay_008048_20 euxassay_008048_21 euxassay_008048_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15020Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15020_wholemount_strong.wlz
15020_wholemount_moderate.wlz
15020_wholemount_weak.wlz
15020_wholemount_possible.wlz
15020_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15020_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Text Annotation:
StructureLevelPatternNotes
liver left lobe
strong strong
regionalstrong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12
liver right lobe
strong strong
regionalstrong expression: see section 13 14 15 16 17 18 19 20 21 22
Annotation Validation: spatial mapping by EMAGE editor, text annotation by author
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36344
Entity Detected:Epb4.2, erythrocyte protein band 4.2 ( MGI:95402)
Sequence:sense strand is shown

>T36344
CAAGAAGCAGTTGTTCATGCTCAAACCCAACCAGGTGATGAGACTATCCACCAACCTGTCCTTCTCCTGT
TTTGAGCAAACCCCACCCGAGAACAGCTTTCTCCGAGTCACGGCAATGGCAAGGTACTCCCACACCAGCC
TCAGCTGCTTTGCCCAGGAAAATATGGCCATTGGTAAACCGGATCTTATCATCGAGATGCCAAAGAGAGC
AGCACAGTATCGGCCCCTCACTGTCTCCGTTAGAATGCACAACTCCTTAGAGGCCCCCATGCAGAACTGC
ATCATCTCCATCTTTGGAAGGGGACTCATTCACAGAGAGAAGAGATATGGATTGGGTTCTCTGTGGCCAG
GAAGCAGCCTACATACTCAGTTCCAGTTCACCCCAACACATCTGGGGCTCCAAAGGCTCACTGTGGAAGT
AGATTGTGACATGTTCCAGAACCTGACCGGCTACAGAAGTGTCCTTGTGGTGGCCCCTGAAGTTTCTGTT
TAACTCCCAAACAACCCTCAACCGTCTAAAACTAGACATGCTCCAGAGGGGAATTCTGCCTACCAAGCAA
CAGACCAGTGGCCAAAAATCAGCTAGATTCACAGAAACCAAAATCCATTTTTTTTCCTTGTTTTTTGGTT
ATTTGGTTGGTTGGTTGGTTGGTATTTTTGAGACAGGGT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74657. Forward Primer - name:074657_F_cDNA_Epb4.2, sequence:CAAGAAGCAGTTGTTCATGCTC; Reverse Primer - name:074657_N_SP6_cDNA_Epb4.2, sequence:ACCCTGTCTCAAAAATACCAACC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15021 same embryo
 EMAGE:15024 same embryo
 EMAGE:15022 same embryo
 EMAGE:15019 same embryo
 EMAGE:15023 same embryo
 EurExpress:euxassay_008048 same experiment
 MGI:4824575 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS