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EMAGE:15021

Ep400 E1A binding protein p400 ( MGI:1276124)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15021 EMAGE:15021 EMAGE:15021 EMAGE:15021 EMAGE:15021
"Pseudo-wholemount" of euxassay_008047. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_008047_01 euxassay_008047_02 euxassay_008047_03 euxassay_008047_04
EMAGE:15021 EMAGE:15021 EMAGE:15021 EMAGE:15021 EMAGE:15021
euxassay_008047_05 euxassay_008047_06 euxassay_008047_07 euxassay_008047_08 euxassay_008047_09
EMAGE:15021 EMAGE:15021 EMAGE:15021 EMAGE:15021 EMAGE:15021
euxassay_008047_10 euxassay_008047_11 euxassay_008047_12 euxassay_008047_13 euxassay_008047_14
EMAGE:15021 EMAGE:15021 EMAGE:15021 EMAGE:15021 EMAGE:15021
euxassay_008047_15 euxassay_008047_16 euxassay_008047_17 euxassay_008047_18 euxassay_008047_19
EMAGE:15021 EMAGE:15021
euxassay_008047_20 euxassay_008047_21

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15021Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15021_wholemount_strong.wlz
15021_wholemount_moderate.wlz
15021_wholemount_weak.wlz
15021_wholemount_possible.wlz
15021_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15021_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: two stars
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T36343
Entity Detected:Ep400, E1A binding protein p400 ( MGI:1276124)
Sequence:sense strand is shown

>T36343
CAACAGGTACAGATGATCCCAACGGTGACTGCAACAGCCCAGCTGGTGCAGCAGAAGCTCATCCAGCAGC
AGGTGGTGACGACAGCCTCAGCATCACTGCAGACCCCTGGTGGCCCAAGCCCAGCACAGCTTCCAGCCAG
CTCTGACAGCCCAAGCCAGCAGCCAAAGTTGCAAATGAGAGTTCCTGCTGTGAGGCTGAAGACGCCCACC
AAACCTCCATGCCAGTAGGATGGAAATTGGTTGATACTATGTTAGGCTGTCCTGGCAGAACTCTATACTG
TTTGTGGACTCTGGGATCTGCTGCCTATAGGGTGGCTGGCTACACAGTTGTTTGACTTTGTCCTGTGTAC
ATGTGACAGTGGCAATCCATGGCAGTGCACAGTTTCTCTTAGTGTGCACAGCTCAGTACTTGGTAGGACT
CCATATCCTACCATTGTTGGATACCACTACTGTTCAGCACATGCATTGTAGTCCTTCAGCAGGAGAGATC
CAAGTCTCTTTCCAGAGCCTCCACTCTGGTCCTCTCTCTTAAAGTGCATATACCCTTCTCAGTATGGCTT
TTGTTACAGGCACCCATCTTGCCCCCGTGAACATTAGCTTTGATTTGAATGATGCTCTGGGGCTGATACC
ATCTCTGTATAGTAAGTTTGTGTTTAGACACATTTTACTAGGCACCTCCGTGGATCCTTTACAGTGTTCT
TGTGACAAGCTAGTGTGGGTCTCAGGTGCTGTAGCCTGGTGGAGTTGAAACCAACGTTACTTTCCCTTAC
TTTTCTCCTGGTCTAGGCTTAGTCTGGGAAGCCTAGTAT
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 65975. Forward Primer - name:065975_F_cDNA_Ep400, sequence:CAACAGGTACAGATGATCCCAA; Reverse Primer - name:065975_N_SP6_cDNA_Ep400, sequence:ATACTAGGCTTCCCAGACTAAGC. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15020 same embryo
 EMAGE:15024 same embryo
 EMAGE:15022 same embryo
 EMAGE:15019 same embryo
 EMAGE:15023 same embryo
 EurExpress:euxassay_008047 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS