EMAGE mouse gene expression spatial database

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EMAGE:15039

Xdh xanthine dehydrogenase ( MGI:98973)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010223. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010223_01	euxassay_010223_02	euxassay_010223_03	euxassay_010223_04

euxassay_010223_05	euxassay_010223_06	euxassay_010223_07	euxassay_010223_08	euxassay_010223_09

euxassay_010223_10	euxassay_010223_11	euxassay_010223_12	euxassay_010223_13	euxassay_010223_14

euxassay_010223_15	euxassay_010223_16	euxassay_010223_17	euxassay_010223_18	euxassay_010223_19

euxassay_010223_20	euxassay_010223_21	euxassay_010223_22

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15039_wholemount_strong.wlz

15039_wholemount_moderate.wlz

15039_wholemount_weak.wlz

15039_wholemount_possible.wlz

15039_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15039_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

adrenal medulla

weak

regional

weak expression: see section 06 07 12 13 14

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37215
Entity Detected:	Xdh, xanthine dehydrogenase ( MGI:98973)
Sequence:	sense strand is shown >T37215 AGAGGAGGTACGGAGGAAAAACATGTACAAAGAAGGGGACCTGACTCACTTCAACCAGAAGCTGGAGGGG TTCACCTTGCCCAGGTGCTGGGATGAGTGCATAGCGAGCTCCCAGTATCAGGCTCGCAAGATGGAAGTGG AGAAATTCAACAGGGAGAACTGTTGGAAAAAGAGAGGGCTGTGTATAATCCCGACTAAGTTTGGAATAAG CTTCACACTTTCTTTTCTGAACCAGGGGGGTGCCTTGGTCCACGTGTACACGGATGGGTCGGTGCTGCTG ACACATGGAGGTACTGAGATGGGTCAAGGCCTTCACACCAAGATGGTTCAGGTGGCCAGCAGAGCTCTGA AAATCCCCACTTCCAAGATCCATATAACGGAGACAAGCACTAACACTGTCCCTAACACCTCTCCCACGGC TGCCTCTGCCAGCGCTGACCTCAATGGCCAGGCCATTTATGAAGCCTGTCAGACCATACTGAAAAGACTG GAGCCCTTCAAGAAAAAGAATCCCTCAGGCTCCTGGGAGAGCTGGGTCATGGATGCCTACACTAGTGCAG TGAGCTTGTCTGCTACTGGATTTTATAAGACACCCAACCTTGGTTACAGCTTTGAGACAAACTCTGGGAA CCCCTTCCACTACTTCAGTTATGGGGTGGCTTG
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 74562. Forward Primer - name:074562_F_cDNA_Xdh, sequence:AGAGGAGGTACGGAGGAAAAAC; Reverse Primer - name:074562_N_SP6_cDNA_Xdh, sequence:CAAGCCACCCCATAACTGAAG. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15044 same embryo
	EMAGE:15043 same embryo
	EMAGE:15040 same embryo
	EMAGE:15042 same embryo
	EMAGE:15041 same embryo
	EurExpress:euxassay_010223 same experiment
	MGI:4829238 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source