Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:15048

Vamp4 vesicle-associated membrane protein 4 ( MGI:1858730)
TS23 (14.5 dpc)
in situ hybridisation

Data Images
EMAGE:15048 EMAGE:15048 EMAGE:15048 EMAGE:15048 EMAGE:15048
"Pseudo-wholemount" of euxassay_010213. This image has been made by aligning and collapsing the constitutent sections. Information about the method. euxassay_010213_01 euxassay_010213_02 euxassay_010213_03 euxassay_010213_04
EMAGE:15048 EMAGE:15048 EMAGE:15048 EMAGE:15048 EMAGE:15048
euxassay_010213_05 euxassay_010213_06 euxassay_010213_07 euxassay_010213_08 euxassay_010213_09
EMAGE:15048 EMAGE:15048 EMAGE:15048 EMAGE:15048 EMAGE:15048
euxassay_010213_10 euxassay_010213_11 euxassay_010213_12 euxassay_010213_13 euxassay_010213_14
EMAGE:15048 EMAGE:15048 EMAGE:15048 EMAGE:15048 EMAGE:15048
euxassay_010213_15 euxassay_010213_16 euxassay_010213_17 euxassay_010213_18 euxassay_010213_19
EMAGE:15048 EMAGE:15048 EMAGE:15048
euxassay_010213_20 euxassay_010213_21 euxassay_010213_22

View assay images: EMAGE genex expression entry
Expression pattern clarity: no stars
Find spatially similar wholemount expression patterns: Find spatially similar wholemount patterns
Expression Pattern Description
Spatial Annotation:
EMAGE:15048Annotation colour key:  
strong strong      
gene expression moderate moderate    
gene expression weak weak        
gene expression possible possible    
gene expression not detected not detected
wholemount mapping

Download individual expression domains:
15048_wholemount_strong.wlz
15048_wholemount_moderate.wlz
15048_wholemount_weak.wlz
15048_wholemount_possible.wlz
15048_wholemount_not_detected.wlz
(what is wlz format?)
Download all expression domains: EMAGE:15048_all_domains.zip
Find spatially similar wholemount expression patterns:  EMAGE spatially similar wholemount patterns
Morphological match to the template: one star
Annotation Validation: spatial mapping by EMAGE editor
Detection Reagent
Type:in situ hybridisation probe
Identifier:T37205
Entity Detected:Vamp4, vesicle-associated membrane protein 4 ( MGI:1858730)
Sequence:sense strand is shown

>T37205
TGGGGTTCCTGTAGACTCTCATTACAGTCCACTCTGGGTTCTGTGTTAGAAAGTAATGCCATTCTGATAG
CAGCAACCGGCACCTTTCTGTTCTGTATGGCATGTGGGTGCCCTCAGATGTGATAAGAGCCTGACCTCTG
TCCTTGTCACTTAACACCTGTTCTGTGTGCTGTCGGCACTGTAGACCAAACAGACTTGTATTTACATCTC
ATAGATATTTTATGGCTAGAAAATATGCAAGAAGTTTTTTTAGTGTTGAAAACAAATGTCATTATATGTT
CTTAGTTGTATTGAGTCAGAGTGTTACGAAGTTAGGAACTGGACTACTAATCAGATTTTGTGTTTTCTCA
CAGAAATGTTATCAGAGTCACTTTTACTTCTCTGTGAGTGATAGTAAGAGCATGACTCAGTCTAAACACA
TGAAGGTGGGGGTTGTCAAATAACAGCAGAAAGGCAGTGGCTCAGAAGACACAGAAAGGGGCCATCTTAG
TCCATACAAATGTGCCTTCCCTTAGACTGACAGGGTCTGAGTTTATCTTGTATGTCTTGGTTCTTTTGGT
TGGTTGGTTGGTTGGTTGGTTGGTTGGTTTTATTTGGTTTTGGTTTTGGCTTTAGTTTTGTTTGTTTGTT
TGTTTGTTTGGATTTTTTTTGTTTTTTTTTTGAGATACGGTCTTACTGTGTGACCCAGACTGACCTCAGA
TGCACACAGAGCCATCAG
Notes:The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 94449. Forward Primer - name:094449_F_cDNA_Vamp4, sequence:TGGGGTTCCTGTAGACTCTCAT; Reverse Primer - name:094449_N_SP6_cDNA_Vamp4, sequence:CTGATGGCTCTGTGTGCATCT. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:RNA
Strand:antisense
Label:digoxigenin
Specimen
Organism:mouse
Strain:C57BL/6
Age:14.5 dpc
Theiler Stage:TS23
Mutations:none (wild-type)
Preparation:section
Procedures
Fixation:none
Embedding:cryosection (OCT)
Staining procedure:alkaline phosphatase + NBT/BCIP
General Information
Authors:Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:screen
References:[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:EMAGE:15050 same embryo
 EMAGE:15049 same embryo
 EMAGE:15046 same embryo
 EMAGE:15047 same embryo
 EMAGE:15045 same embryo
 EurExpress:euxassay_010213 same experiment
  Ensembl same gene
  Allen Brain Atlas same gene
  BioGPS same gene
  International Mouse Knockout Project Status same gene
  GEISHA Chicken ISH Database same gene
  EMBL-EBI Gene Expression Atlas same gene
  BrainStars same gene
  ViBrism same gene
Data SourceEUREXPRESS