EMAGE mouse gene expression spatial database

Quicksearch Help

(Click the icon to keep this page displayed.)

EMAGE:15090

Flnc filamin C, gamma ( MGI:95557)
TS23 (14.5 dpc)
in situ hybridisation

Data Images


"Pseudo-wholemount" of euxassay_010297. This image has been made by aligning and collapsing the constitutent sections. Information about the method.	euxassay_010297_01	euxassay_010297_02	euxassay_010297_03	euxassay_010297_04

euxassay_010297_05	euxassay_010297_06	euxassay_010297_07	euxassay_010297_08	euxassay_010297_09

euxassay_010297_10	euxassay_010297_11	euxassay_010297_12	euxassay_010297_13	euxassay_010297_14

euxassay_010297_15	euxassay_010297_16	euxassay_010297_17	euxassay_010297_18	euxassay_010297_19

euxassay_010297_20	euxassay_010297_21	euxassay_010297_22	euxassay_010297_23

View assay images:
Expression pattern clarity:
Find spatially similar wholemount expression patterns:

Expression Pattern Description

Spatial Annotation:

Annotation colour key:

	strong
	moderate
	weak
	possible
	not detected

wholemount mapping

Download individual expression domains:

15090_wholemount_strong.wlz

15090_wholemount_moderate.wlz

15090_wholemount_weak.wlz

15090_wholemount_possible.wlz

15090_wholemount_not_detected.wlz

(what is wlz format?)

Download all expression domains:

EMAGE:15090_all_domains.zip

Find spatially similar wholemount expression patterns:

EMAGE spatially similar wholemount patterns

Morphological match to the template:

Text Annotation:

Structure

Level

Pattern

Notes

upper arm muscle

strong

regional

strong expression: see section 01 02 03 04 05 19 20 21 22 23

upper leg muscle

strong

regional

strong expression: see section 03 04 05 19 20 21 22 23

diaphragm

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 15 16 17 18 19 20 21 22 23

forearm rest of mesenchyme

strong

regional

strong expression: see section 01 02 03 22 23

lower leg rest of mesenchyme

strong

regional

strong expression: see section 01 03 22 23

vertebral axis musculature

strong

regional

strong expression: see section 01 02 03 04 05 06 07 08 09 10 11 12 13 14 15 16 17 18 19 20 21 22 23

spinal cord ventricular layer

moderate

regional

moderate expression: see section 10 11 weak expression: see section 12

extrinsic ocular muscle

strong

regional

strong expression: see section 06 07 08 22 23 moderate expression: see section 20 weak expression: see section 21

tongue muscle

strong

regional

strong expression: see section 10 11 12 13 14 15 16

tail mesenchyme

strong

regional

strong expression: see section 13 14 15 16 17 18 19

Annotation Validation:

spatial mapping by EMAGE editor, text annotation by author

Detection Reagent

Type:	in situ hybridisation probe
Identifier:	T37237
Entity Detected:	Flnc, filamin C, gamma ( MGI:95557)
Sequence:	sense strand is shown >T37237 CACTGTCAAGGAGAAGGGAGACTACATCCTCATTGTCAAGTGGGGTGATGAAAGTGTCCCTGGGAGCCCC TTCAAGGTCAATGTGCCCTGAATCTCAGAAGTATTGCCCCCCAGACCAGGCCCCCACTTCCTGCCAAACT CATACCCACACAGATGTGCCACACCCCTCTGCACACTCACAGAATCAGACACTAAAACACCTGCCTTAGG TGTGATGTGAGTGGTACAACCCCCCCCCCCCCGTGTCACTTGAGGGGTGGGGGGCCTTTATCTGTCTCAG ATCAGTGTCCCTCAAATGTGGCCCAAGGGCAGACTGGGGTGAGGCTCCGGGCAGAGACTGTGAAATAAGG GGTTAAACAGGGGTTGGGGGAAGCCTTGGGGGTCATAGTGGGGCTGATGCCAGTGGGCAAGCACTGAGTT GGAGGATCTGTGTGAGAGAGAAGTCACCCTCACAAACCGCCGTGCCTAAGGACTCTGTCAGGC
Notes:	The probe template was received by the EURExpress consortium as an aliquot of PCR product from the Allen Brain Atlas (originally amplified from wild type C57BL/6J mouse whole brain cDNA), and this was re-amplified using the gene-specific Allen Brain Atlas Primer Pair 79308. Forward Primer - name:079308_F_cDNA_1110055E19Rik, sequence:CACTGTCAAGGAGAAGGGAGAC; Reverse Primer - name:079308_N_SP6_cDNA_1110055E19Rik, sequence:GCCTGACAGAGTCCTTAGGCA. The reverse primer contains a 5' extension containing an Sp6 RNA polymerase binding site (this extension is not shown in the primer sequence listed here). Anti-sense probe was transcribed from the PCR amplified template using Sp6 polymerase.
Chemistry:	RNA
Strand:	antisense
Label:	digoxigenin

Specimen

Organism:	mouse
Strain:	C57BL/6
Age:	14.5 dpc
Theiler Stage:	TS23
Mutations:	none (wild-type)
Preparation:	section

Procedures

Fixation:	none
Embedding:	cryosection (OCT)
Staining procedure:	alkaline phosphatase + NBT/BCIP

General Information

Authors:	Dr. Graciana Diez-Roux, Prof. Gregor Eichele, Prof. Richard Baldock, Dr. Duncan Davidson, Prof. Stylianos Antonarakis, Dr. Marie Laure Yaspo, Prof. Salvador Martinez Perez, Dr. Pascal Dolle, Dr. David Tannahill, Prof. Pier Paolo Di Fiore, Mr. Stefan Kruse, Mr. Paolo Sarmientos, Dr. Uwe Radelof, Prof. Andrea Ballabio.
Principal investigator:	Professor Andrea Ballabio, Fondazione Telethon, TIGEM Instituto di Genetica e Medicina, Via Pietro Castellino 111 80131, Napoli, Italy 80131
Submitted by:	EMAGE EDITOR, Institute of Genetics and Molecular Medicine, Western General Hospital, Crewe Road, Edinburgh, UK EH4 2XU
Experiment type:	screen
References:	[ doi:10.1371/journal.pbio.1000582] [ PMID:21267068] Diez-Roux G, Banfi S, Sultan M, Geffers L, Anand S, Rozado D, et al 2011 A High-Resolution Anatomical Atlas of the Transcriptome in the Mouse Embryo PLoS Biol (9)
Links:	EMAGE:15089 same embryo
	EMAGE:15091 same embryo
	EurExpress:euxassay_010297 same experiment
	MGI:4824878 same experiment
	Ensembl same gene
	Allen Brain Atlas same gene
	BioGPS same gene
	International Mouse Knockout Project Status same gene
	GEISHA Chicken ISH Database same gene
	EMBL-EBI Gene Expression Atlas same gene
	BrainStars same gene
	ViBrism same gene
Data Source